Journal of Integrative Agriculture ›› 2015, Vol. 14 ›› Issue (5): 984-987.DOI: 10.1016/S2095-3119(14)60911-4

• 论文 • 上一篇    

Complete genome sequence analysis of two Citrus tatter leaf virus (CTLV) isolates from China

 SONG Zhen, LI Zhong-an, LIU Ke-hong, ZHOU Chang-yong   

  1. Citrus Research Institute, Southwest University/Chinese Academy of Agricultural Sciences, Chongqing 400712, P.R.China
  • 收稿日期:2014-08-08 出版日期:2015-05-01 发布日期:2015-05-13
  • 通讯作者: ZHOU Chang-yong, Tel: +86-23-68349601,E-mail: zhoucy@cric.cn
  • 作者简介:SONG Zhen, Tel: +86-23-68349017, E-mail: songzhen@cric.cn;
  • 基金资助:

    This work was supported by the Special Fund for Agro-Scientific Research in the Public Interest of China (201203076), the 111 Project, China (B12006), the Fundamental Research Funds for the Central Universities, China (XDJK2013C161, SWU113097), and the Basic and Frontier Research Programs of Chongqing, China (CSTC2014jcyjA80033).

Complete genome sequence analysis of two Citrus tatter leaf virus (CTLV) isolates from China

 SONG Zhen, LI Zhong-an, LIU Ke-hong, ZHOU Chang-yong   

  1. Citrus Research Institute, Southwest University/Chinese Academy of Agricultural Sciences, Chongqing 400712, P.R.China
  • Received:2014-08-08 Online:2015-05-01 Published:2015-05-13
  • Contact: ZHOU Chang-yong, Tel: +86-23-68349601,E-mail: zhoucy@cric.cn
  • About author:SONG Zhen, Tel: +86-23-68349017, E-mail: songzhen@cric.cn;
  • Supported by:

    This work was supported by the Special Fund for Agro-Scientific Research in the Public Interest of China (201203076), the 111 Project, China (B12006), the Fundamental Research Funds for the Central Universities, China (XDJK2013C161, SWU113097), and the Basic and Frontier Research Programs of Chongqing, China (CSTC2014jcyjA80033).

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摘要： In order to understand molecular characterization of Citrus tatter leaf virus (CTLV) isolated from China, full-length cDNAs of CTLV-MTH and CTLV-XHC from Citrus reticulata and Citrus sinensis were cloned and sequenced based on whole-genome amplification by RT-PCR. The complete nucleotide sequences of CTLV-MTH and CTLV-XHC were determined to be 6 497 nucleotides in length and shared 79.9–91.0% and 78.8–98.0% nucleotide sequence identity, respectively, with other Apple stem grooving virus (ASGV) or CTLV strains available in GenBank. Unexpectedly, CTLV-MTH showed the highest nucleotide sequence identity (91%) with an apple isolate of ASGV, followed by 86.5% with ASGV-HH and 85.7% with ASGV-CHN. Furthermore, CTLV-MTH and three ASGV strains were grouped to a separate cluster in the phylogenetic tree, suggesting it has a closer relationship to ASGV than to CTLV. Therefore, it can be concluded roughly that CTLV may be not a distinct strains of ASGV. We proposed that Citrus tatter leaf virus should be renamed Apple stem grooving virus.

关键词: Citrus tatter leaf virus , Apple stem grooving virus , phylogenetic analysis , genome

Abstract: In order to understand molecular characterization of Citrus tatter leaf virus (CTLV) isolated from China, full-length cDNAs of CTLV-MTH and CTLV-XHC from Citrus reticulata and Citrus sinensis were cloned and sequenced based on whole-genome amplification by RT-PCR. The complete nucleotide sequences of CTLV-MTH and CTLV-XHC were determined to be 6 497 nucleotides in length and shared 79.9–91.0% and 78.8–98.0% nucleotide sequence identity, respectively, with other Apple stem grooving virus (ASGV) or CTLV strains available in GenBank. Unexpectedly, CTLV-MTH showed the highest nucleotide sequence identity (91%) with an apple isolate of ASGV, followed by 86.5% with ASGV-HH and 85.7% with ASGV-CHN. Furthermore, CTLV-MTH and three ASGV strains were grouped to a separate cluster in the phylogenetic tree, suggesting it has a closer relationship to ASGV than to CTLV. Therefore, it can be concluded roughly that CTLV may be not a distinct strains of ASGV. We proposed that Citrus tatter leaf virus should be renamed Apple stem grooving virus.

Key words: Citrus tatter leaf virus , Apple stem grooving virus , phylogenetic analysis , genome