An Inner Membrane Protein (Imp) of Xanthomonas oryzae pv. oryzicola Functions in Carbon Acquisition, EPS Production, Bacterial Motility and Virulence in Rice

doi:10.1016/S2095-3119(14)60915-1

Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (12): 2656-2668.DOI: 10.1016/S2095-3119(14)60915-1

An Inner Membrane Protein (Imp) of Xanthomonas oryzae pv. oryzicola Functions in Carbon Acquisition, EPS Production, Bacterial Motility and Virulence in Rice

CAI Lu-lu, ZOU Li-fang, GE Ling, XUE Xiao-bo, ZOU Hua-song , CHEN Gong-you

School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, P.R.China

收稿日期:2013-12-26 出版日期:2014-12-01 发布日期:2014-12-10
通讯作者: CHEN Gong-you, Tel/Fax: +86-21-34205873, E-mail: gyouchen@sjtu.edu.cn
作者简介:CAI Lu-lu, E-mail: cailulusjtu@gmail.com
基金资助:
Acknowledgements This work was supported by the Ministry of Agriculture of China (201303015), the Key Basic Research Project of Shanghai Committee of Science and Technology, China (11JC1406300), and the Ph D Programs Foundation of Ministry of Education of China (20100073110045).

An Inner Membrane Protein (Imp) of Xanthomonas oryzae pv. oryzicola Functions in Carbon Acquisition, EPS Production, Bacterial Motility and Virulence in Rice

CAI Lu-lu, ZOU Li-fang, GE Ling, XUE Xiao-bo, ZOU Hua-song , CHEN Gong-you

School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, P.R.China

Received:2013-12-26 Online:2014-12-01 Published:2014-12-10
Contact: CHEN Gong-you, Tel/Fax: +86-21-34205873, E-mail: gyouchen@sjtu.edu.cn
About author:CAI Lu-lu, E-mail: cailulusjtu@gmail.com
Supported by:
Acknowledgements This work was supported by the Ministry of Agriculture of China (201303015), the Key Basic Research Project of Shanghai Committee of Science and Technology, China (11JC1406300), and the Ph D Programs Foundation of Ministry of Education of China (20100073110045).

摘要/Abstract

摘要： Xanthomonas oryzae pv. oryzicola (Xoc) causes bacterial leaf streak, a devastating disease in rice-growing regions worldwide. A Tn5-insertion mutant in Xoc_3248, encoding an inner membrane protein (Imp), showed reduced virulence in rice. To explore the potential function of this gene in virulence, a deletion mutant RΔimp was constructed in the wild-type RS105. The RΔimp mutant was significantly impaired for bacterial virulence and growth in planta. The mutation in imp made the pathogen insufficiently utilize glucose, fructose, mannose or pyruvate as a sole carbon source, leading to less extracellular polysaccharide (EPS) production and reduced motility. The deficiencies noted for the mutant were restored to wild-type levels when imp was introduced in trans. Transcription of imp was significantly declined when hrpG and hrpX was mutated and the expression of hrpG and hrpX was also significantly declined when imp was deleted. Cell sublocalization in planta showed Imp membrane-binding feature. These results suggest that Imp is a virulence factor with roles in the catabolism of sugars, EPS production, and bacterial motility.

关键词: Xanthomonas oryzae pv. oryzicola , inner membrane protein , extracellular polysaccharide , motility , virulence

Abstract: Xanthomonas oryzae pv. oryzicola (Xoc) causes bacterial leaf streak, a devastating disease in rice-growing regions worldwide. A Tn5-insertion mutant in Xoc_3248, encoding an inner membrane protein (Imp), showed reduced virulence in rice. To explore the potential function of this gene in virulence, a deletion mutant RΔimp was constructed in the wild-type RS105. The RΔimp mutant was significantly impaired for bacterial virulence and growth in planta. The mutation in imp made the pathogen insufficiently utilize glucose, fructose, mannose or pyruvate as a sole carbon source, leading to less extracellular polysaccharide (EPS) production and reduced motility. The deficiencies noted for the mutant were restored to wild-type levels when imp was introduced in trans. Transcription of imp was significantly declined when hrpG and hrpX was mutated and the expression of hrpG and hrpX was also significantly declined when imp was deleted. Cell sublocalization in planta showed Imp membrane-binding feature. These results suggest that Imp is a virulence factor with roles in the catabolism of sugars, EPS production, and bacterial motility.

Key words: Xanthomonas oryzae pv. oryzicola , inner membrane protein , extracellular polysaccharide , motility , virulence

CAI Lu-lu, ZOU Li-fang, GE Ling, XUE Xiao-bo, ZOU Hua-song , CHEN Gong-you. An Inner Membrane Protein (Imp) of Xanthomonas oryzae pv. oryzicola Functions in Carbon Acquisition, EPS Production, Bacterial Motility and Virulence in Rice[J]. Journal of Integrative Agriculture, 2014, 13(12): 2656-2668.

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An Inner Membrane Protein (Imp) of Xanthomonas oryzae pv. oryzicola Functions in Carbon Acquisition, EPS Production, Bacterial Motility and Virulence in Rice

An Inner Membrane Protein (Imp) of Xanthomonas oryzae pv. oryzicola Functions in Carbon Acquisition, EPS Production, Bacterial Motility and Virulence in Rice

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