Scientia Agricultura Sinica ›› 2008, Vol. 41 ›› Issue (6): 1575-1582 .doi: 10.3864/j.issn.0578-1752.2008.06.002

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Analysis of specific binding and subcellular localization of wheat ERF transcription factor W17

Yun-Xiang ZHAO Zhao-shi XU Ming CHEN Lian-Cheng LI Yao-Feng CHEN You-Zhi MA   

  1. 西北农林科技大学农学院
  • Received:2007-03-12 Revised:1900-01-01 Online:2008-06-10 Published:2008-06-10
  • Contact: Yun-Xiang ZHAO

Abstract: 【 Objective】The study aims to detect the subcellular localization of ERF(ethylene-responsive element binding factor)transcription factor W17 protein, the interaction between W17 and cis-acting regulatory elements GCC box and DRE in vitro, and the role of W17 in higher plant stress-signal pathway. 【Methods】Recombinant plasmid W17/163hGFP was introduced into onion epidermal cells by the particle bombardment method with a PDS1000/He. Transformed cells were incubated for 24 h at 22 ℃ in the dark and green fluorescence was monitored under a confocal microscope. The gene W17 was fused N-terminus of GST (glutathione-S-transferase) in prokaryotic expression vector pGEX-4T-1 and then transformed into E. coli strain BL21 (DE3). IPTG (0.5 mmol•L-1) was added to induce the expression of recombinant GST/W17 for 3 h.. The fused proteins were purified by GST purification columns, and then subjected to gel retardation assay with a 32P-labeled GCC or DRE sequence.【Results】The GFP fused with W17 protein were localized in the neclei; SDS-PAGE demonstrated that the fused protein could be induced and purified with molecular weight around 42.2 kD under the induction of IPTG. Purified fused protein was able to specifically bind to both the wild-type GCC box and DRE element, but had no interaction with either the mutant DRE or GCC box. 【Conclusion】W17 can localize into the nuclei and may be involved not only in pathogen- (biotic stress), but also in salt- (abiotic stress) induced signaling pathway.

Key words: ERF/AP2 domain, ERF, DRE element, GCC-box, subcellular localization

[1] FANG HaoYuan, YANG Liang, WANG HongZhuang, CAO JinCheng, REN WanPing, WEI ShengJuan, YAN PeiShi. Effects of Cross-Ventilation System on Physiology and Production Performance of Beef Cattle in Summer [J]. Scientia Agricultura Sinica, 2022, 55(5): 1025-1036.
[2] CHE DaLu,ZHAO LiChen,CHENG SuCai,LIU AiYu,LI XiaoYu,ZHAO ShouPei,WANG JianCheng,WANG Yuan,GAO YuHong,SUN XinSheng. Effect of Litter Bed on Growth Performance and Odor Emission in Fattening Lamb [J]. Scientia Agricultura Sinica, 2022, 55(24): 4943-4956.
[3] GUO ShaoLei,XU JianLan,WANG XiaoJun,SU ZiWen,ZHANG BinBin,MA RuiJuan,YU MingLiang. Genome-Wide Identification and Expression Analysis of XTH Gene Family in Peach Fruit During Storage [J]. Scientia Agricultura Sinica, 2022, 55(23): 4702-4716.
[4] ZHANG Rui,ZHANG XueYao,ZHAO XiaoMing,MA EnBo,ZHANG JianZhen. Antibody Preparation and Subcellular Localization of LmKnk3-5′ in Locusta migratoria [J]. Scientia Agricultura Sinica, 2022, 55(2): 329-338.
[5] LIU WangJing,TANG DeFu,AO ChangJin. Effect of Allium mongolicum Regel and Its Extracts on the Growth Performance, Carcass Characteristics, Meat Quality and Serum Biochemical Indices of Captive Small-Tailed Han Sheep [J]. Scientia Agricultura Sinica, 2022, 55(17): 3461-3472.
[6] HAN ShouWei,SI JiSheng,YU WeiBao,KONG LingAn,ZHANG Bin,WANG FaHong,ZHANG HaiLin,ZHAO Xin,LI HuaWei,MENG Yu. Mechanisms Analysis on Yield Gap and Nitrogen Use Efficiency Gap of Winter Wheat in Shandong Province [J]. Scientia Agricultura Sinica, 2022, 55(16): 3110-3122.
[7] GUAN RuoBing,LI HaiChao,MIAO XueXia. Commercialization Status and Existing Problems of RNA Biopesticides [J]. Scientia Agricultura Sinica, 2022, 55(15): 2949-2960.
[8] YIN Fei,LI ZhenYu,SAMINA Shabbir,LIN QingSheng. Expression and Function Analysis of Cytochrome P450 Genes in Plutella xylostella with Different Chlorantraniliprole Resistance [J]. Scientia Agricultura Sinica, 2022, 55(13): 2562-2571.
[9] CHEN ZhiMin,CHANG WenHuan,ZHENG AiJuan,CAI HuiYi,LIU GuoHua. Effect of Expanded Feather Powder on Growth Performance, Slaughter Performance and Serum Biochemical Index of Broiler [J]. Scientia Agricultura Sinica, 2022, 55(13): 2643-2653.
[10] RAN HongBiao,ZHAO LiLing,WANG Hui,CHAI ZhiXin,WANG JiKun,WANG JiaBo,WU ZhiJuan,ZHONG JinCheng. Effects of lncFAM200B on the Lipid Deposition in Intramuscular Preadipocytes of Yak [J]. Scientia Agricultura Sinica, 2022, 55(13): 2654-2666.
[11] WU Wei,XU HuiLi,WANG ZhengLiang,YU XiaoPing. Cloning and Function Analysis of a Serine Protease Inhibitor Gene Nlserpin2 in Nilaparvata lugens [J]. Scientia Agricultura Sinica, 2022, 55(12): 2338-2346.
[12] CHEN ErHu,MENG HongJie,CHEN Yan,TANG PeiAn. Cuticle Protein Genes TcCP14.6 and TcLCPA3A are Involved in Phosphine Resistance of Tribolium castaneum [J]. Scientia Agricultura Sinica, 2022, 55(11): 2150-2160.
[13] HAN Xiao, YANG HangYu, CHEN WeiKai, WANG Jun, HE Fei. Effects of Different Rootstocks on Flavonoids of Vitis vinifera L. cv. Tannat Grape Fruits [J]. Scientia Agricultura Sinica, 2022, 55(10): 2013-2025.
[14] YAN Ya,WANG GuangWen,KONG FanDi,WANG XuYuan,WANG YiHan,LI JunPing,ZHAO YuHui,LI ChengJun,CHEN HuaLan,JIANG Li. Mechanism of NMRAL1 Regulating Influenza Virus Replication [J]. Scientia Agricultura Sinica, 2022, 55(10): 2067-2076.
[15] QIN HongDe, FENG ChangHui, ZHANG YouChang, BIE Shu, ZHANG JiaoHai, XIA SongBo, WANG XiaoGang, WANG QiongShan, LAN JiaYang, CHEN QuanQiu, JIAO ChunHai. F1 Performance Prediction of Upland Cotton Based on Partial NCII Design [J]. Scientia Agricultura Sinica, 2021, 54(8): 1590-1598.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!