Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (11): 2303-2309 .doi: 10.3864/j.issn.0578-1752.2011.11.012

• HORTICULTURE • Previous Articles     Next Articles

Effects of Vernalization on Carbohydrate Content and Sugar-Metabolizing Enzyme Activities in Radish (Raphanus sativus L.)

DU Xian-yun, CHENG Ji-hong, YANG Rui, LEI Hui, YANG Liu, WANG Shao-hui   

  1. 北京农学院植物科学技术学院/北京市农业应用新技术重点实验室
  • Received:2010-07-30 Revised:2011-03-02 Online:2011-06-01 Published:2011-06-01

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Abstract:

【Objective】The aim of this paper was to investigate the effect of vernalization on carbohydrate content and sucrose metabolizing enzyme activities of radish (Raphanus sativus L.). 【Method】Radish variety ‘Beinong No.1’was vernalized (treatment at 4℃ for 30 days) after germination. Mature leaves (source) and taproot (sink) were sampled to investigate carbohydrate content and sucrose metabolizing enzyme activities.【Result】After vernalization, soluble sugar content in leaves and fleshy taproot both decreased, starch content increased. The activity of NI (neutral invertase) in leaves of vernalized radish increased from two-leaf stage to the 21st day after two-leaf stage, 28th day later, NI activity decreased and lower than that of control. The activities of SS (sucrose synthase) and SPS (sucrose phosphate synthase) were always lower than that of control during the tested period. The activity of NI and SPS in vernalized taproot decreased, whereas, neither the activity of AI (acid invertase) nor SPS had significant differences compared with that of control.【Conclusion】After vernalization, the carbohydrate metabolism changes and all soluble sugar content were declined in both leaves and taproot of ‘Beinong No.1’, vernalization influenced the activity of invertase, SS and SPS which of great importance in sucrose synthesis and degradation transport from source to skin in leaves and root. The reduced concentration of soluble sugar may be contributed to the restricted growth of the flesh taproot after vernalization.

Key words: Radish, Vernalization, Carbohydrate of source and sink, Sucrose Metabolizing Enzyme

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