Abstract:

【Objective】 This study aims to isolate a new transcription factor related to cotton fiber development from the fuzzless cotton mutant GZnn and investigate its transcriptional activation and expression pattern in PGhMS3:GUS transgenic tobacco plants. 【Method】 GhMS3 cDNA and genomic DNA sequences were obtained by RACE (rapid amplification of the cDNA ends) and genome walking. Bioinformatics methods were used to analyze the DNA and the encoded amino acid sequence. The transcriptional activation in yeast cells and the expression pattern in PGhMS3:GUS transgenic tobacco plants were analyzed, respectively. 【Result】The full length of GhMS3 genomic DNA and a 1 174 bp upstream region were isolated. GhMS3,analyzed by amino acid alignment, was a R2R3 MYB transcription factor. Yeast one-hybrid assay showed that the C terminus of GhMS3 had strong expression in trichomes, root hairs, and actively dividing cells of PGhMS3:GUS transgenic tobacco plants. 【Conclusion】 GhMS3, a R2R3 MYB transcriptions factor gene cloned from fuzzless cotton mutant GZnn, was specifically expressed during ovule development of GZnn and in actively dividing cells in PGhMS3:GUS transgenic tobacco plants. GhMS3 had transcriptional activation in vitro. Further function analysis of GhMS3 in plant cell differentiation is undergoing.

Key words: cotton transcription factor, GhMS3, transcriptional activation, GUS staining