Abstract:

【Objective】 The objective of the experiment was to study the antioxidative activity of viable cells and heat-killing cells of Lactobacillus casei subsp.casei SY13 on aged model mice induced by D-galactose. 【Method】 ICR subacute aged model mice, which were given subcutaneous injection of D-galactose continuously, were fed with viable cells and heat-killing cells of Lactobacillus casei subsp.casei SY13. The content of malondialdehyde (MDA), activity of superoxide dismutase (SOD) , glutathione peroxidase (GSH-Px) and total antioxidant capacity (T-AOC) in serum and liver of the mice were tested. 【Result】 SY13 viable cells high dose group and middle dose group(20,10 mL•kg-1) could obviously reduce the aged mice serum and liver tissue MDA content , enhance evidently the GSH-Px activity and total antioxidant capacity (T-AOC), but the SOD activity did not change significantly.【Conclusion】Lactobacillus casei subsp. casei SY13 viable cells can obviously increase the antioxidant ability of ageing mice and has certain anti-aging effects.

Key words: Lactobacillus casei, D-galactose, aged mice, antioxidative activity in vivo