17beta-雌二醇通过beta受体和cAMP-ERK1/2级联调节仔猪睾丸支持细胞cyclinA2 mRNA的表达

doi:10.3864/j.issn.0578-1752.2011.10.019

Abstract

Abstract: 【Objective】 The objective of this study was to identify whether 17beta-estradiol regulates the expression of cCyclinA2 mRNA via the estrogen receptor beta (ERbetβa) and the cAMP-PKA-extracellular signal-regulated kinase (ERK1/2) pathway. 【Method】Cultured immature boar sertoli cells were treated with 10-9 mol•L-1 17beta-estradiol, and real-time PCR was used to detect the expression of cyclinA2 mRNA.【Result】Treatment with 17beta-estradiol increased the expression of cyclinA2 mRNA from 15 min to 90 min (P＜0.05). The effects of 17beta-estradiol activity peaked at 30 min compared to the control cells (P＜0.05). Combined treatment with ICI182780 and ERβ reduced the 17beta-estradiol-induced increase in the expression of cyclinA2 mRNA (P＜0.05), but ERβ alone did not significantly affect these parameters (P＜0.05). Both 17beta-estradiol and forskolin induced the abundance of cyclinA2 mRNA (P＜0.05 for both). Combined treatment with Rp-cAMP, H-89 and U0126 reduced the 17beta -estradiol-induced expression of cyclinA2 mRNA (P＜0.05 for all), but Rp-cAMP, H-89 and U0126 alone had no significant effect on the abundance of cyclinA2 mRNA, compared to the control group.【Conclusion】 This study showed that 17beta-estradiol regulates the expression of cyclinA2 mRNA via the activation of ERβ, cAMP-PKA and ERK1/2.

Key words: 17beta-estradiol, sertoli cell, cyclinA2, estrogen receptor, ERK1/2

ZUO Jing, GAN Rui, ZHANG Guo-Sheng, ZHANG Jiao-Jiao, ZHU Feng-Wei, SUN Yan, WANG Xian-Zhong, ZHANG Jia-Hua. 17beta-Estradiol Regulates the Expression of CyclinA2 mRNA of Cultured Immature Boar Sertoli Cells via Estrogen Receptor beta, cAMP-PKA and ERK1/2[J].Scientia Agricultura Sinica, 2011, 44(10): 2139-2145.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.chinaagrisci.com/EN/10.3864/j.issn.0578-1752.2011.10.019

https://www.chinaagrisci.com/EN/Y2011/V44/I10/2139

References

[1]Wood M A, Walker W H. USF1/2 transcription factor DNA-binding activity is induced during rat sertoli cell differentiation. Biology of Reproduction, 2009, 80: 24-33.

[2]Franca L R, Jr Silva V A, Chiarini-Garcia H, Garcia S K, Debeljuk L. Cell proliferation and hormonal changes during postnatal development of the testis in the pig. Biology of Reproduction, 2000, 63: 1629-1636.

[3]Malumbres M, Barbacid M. Mammalian cyclin-dependent kinases. Trends in Biochemical Sciences, 2005, 30: 630-641.

[4]Hwang H C, Clurman B E. Cyclin E in normal and neoplastic cell cycles. Oncogene, 2005, 24: 2776-2786.

[5]Spengler M L, Guo L W, Brattain M G. Phosphorylation mediates Sp1 coupled activities of proteolytic processing, desumoylation and degradation. Cell Cycle, 2008, 7: 623-630.

[6]Brown N R, Lowe E D, Petri E, Skamnaki V, Antrobus R, Johnson L N. Cyclin B and cyclin A confer different substrate recognition properties on CDK2. Cell Cycle, 2007, 6: 1350-1359.

[7]Lucas T F, Siu E R, Esteves C A, Monteiro H P, Oliveira C A, Porto C S, Lazari M F. 17beta-estradiol induces the translocation of the estrogen receptors ESR1 and ESR2 to the cell membrane, MAPK3/1 phosphorylation and proliferation of cultured immature rat sertoli cells. Biology of Reproduction, 2008, 78: 101-114.

[8]Wang X Z, Zhao B C, Zhou Y L, Zhou Y T, Ma K G, Zhang J H. 17 beta-estradiol regulates cultured immature boar sertoli cell proliferation via the cAMP-ERK1/2 pathway and the estrogen receptor beta. Agricultural Sciences in China, 2010, 9: 1201-1210.

[9]Chambard J C, Lefloch R, Pouyssegur J, Lenormand P. ERK implication in cell cycle regulation. Biochimica et Biophysica Acta, 2007, 1773: 1299-1310.

[10]Benbrahim-Tallaa L, Boussouar F, Rey C, Benahmed M. Tumor necrosis factor-alpha inhibits glutathione S-transferase-alpha expression in cultured porcine sertoli cells. Journal of Endocrinology, 2002, 175: 803-812.

[11]McCoard S A, Lunstra D D, Wise T H, Ford J J. Specific staining of sertoli cell nuclei and evaluation of sertoli cell number and proliferative activity in Meishan and White composite boars during the neonatal period. Biology of Reproduction, 2001, 64: 689-695.

[12]Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods, 2001, 25: 402-408.

[13]赵伯川, 程舒巧, 周玉兰, 周银涛, 王鲜忠, 张家骅. 17β-雌二醇对仔猪支持细胞周期的影响. 中国畜牧杂志, 2010, 46(13): 19-22.

Zhao B C, Cheng S Q, Zhou Y L , Zhou Y T , Wang X Z , Zhang J H. Effect of 17β- estradiol on pigle sertoli cell cycle. Chinese Journal of Animal Science, 2010, 46(13): 19-22. (in Chinese)

[14]王鲜忠, 周玉兰, 赵伯川, 周银涛, 陈永军, 张姣姣, 王怡, 张家骅. FSH通过cAMP、Ca2+调节仔猪睾丸支持细胞的增殖. 畜牧兽医学报, 2010, 41: 1240-1245.

Wang X Z, Zhou Y L, Zhao B C, Zhou Y T, Cheng Y J, Zhao J J, Wang Y, Zhang J H. FSH regulates boar sertoli cell proliferation via cAMP and Ca2+ influx. Acta Veterinaria et Zootechnica Sinica, 2010, 41: 1240-1245. (in Chinese)

[15]Chowdhury R, Chatterjee R, Giri A K, Mandal C, Chaudhuri K. Arsenic-induced cell proliferation is associated with enhanced ROS generation, Erk signaling and cyclinA expression. Toxicology Letters, 2010, 198: 263-271.

[16]Chen J H, Tseng T H, Ho Y C, Lin H H, Lin W L, Wang C J. Gaseous nitrogen oxides stimulate cell cycle progression by retinoblastoma phosphorylation via activation of cyclins/Cdks [correction]. Toxicology Letters, 2003, 76: 83-90.

[17]Atanassova N N, Walker M, McKinnell C, Fisher JS, Sharpe RM. Evidence that androgens and oestrogens, as well as follicle- stimulating hormone, can alter sertoli cell number in the neonatal rat. Journal of Endocrinology, 2005, 184: 107-117.

[18]Wade C B, Dorsa D M. Estrogen activation of cyclic adenosine 5'-monophosphate response element-mediated transcription requires the extracellularly regulated kinase/mitogen-activated protein kinase pathway. Endocrinology, 2003, 144: 832-838.

[19]Bouskine A, Nebout M, Brucker-Davis F, Benahmed M, Fenichel P. Low doses of bisphenol A promote human seminoma cell proliferation by activating PKA and PKG via a membrane G-protein-coupled estrogen receptor. Environmental Health Perspectives, 2009, 117: 1053-1058.

[20]Moulager M, Corellou F, Verge V, Escande M L, Bouget F Y. Integration of light signals by the retinoblastoma pathway in the control of S phase entry in the picophytoplanktonic cell ostreococcus. PLoS Genetics, 2010. DOI: 10.1371/journal.pgen.1000957.

[21]Makkonen K M, Malinen M, Ropponen A, Vaisanen S, Carlberg C. Cell cycle regulatory effects of retinoic acid and forskolin are mediated by the cyclin C gene. Journal of Molecular Biology, 2009, 393: 261-271.

[22]Aronica S M, Kraus W L, Katzenellenbogen B S. Estrogen action via the cAMP signaling pathway: stimulation of adenylate cyclase and cAMP-regulated gene transcription. Proceedings of the National Academy of Sciences, 1994, 91: 8517-8521.

[23]Bjornstrom L, Sjoberg M. Mechanisms of estrogen receptor signaling: convergence of genomic and nongenomic actions on target genes. Molecular Endocrinology, 2005, 19: 833-842.

[24]Villanueva J, Yung Y, Walker J L, Assoian R K. ERK activity and G1 phase progression: identifying dispensable versus essential activities and primary versus secondary targets. Molecular Biology of the Cell, 2007, 18: 1457-1463.

[25]Katz M, Amit I, Yarden Y. Regulation of MAPKs by growth factors and receptor tyrosine kinases. Biochimica et Biophysica Acta, 2007, 1773: 1161-1176.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

17beta-Estradiol Regulates the Expression of CyclinA2 mRNA of Cultured Immature Boar Sertoli Cells via Estrogen Receptor beta, cAMP-PKA and ERK1/2

PDF

Cited

Abstract

Cite this article

share this article

References

Related Articles 3

Metrics

Comments

Recommended 0

[1]	ZHANG Jiao-Jiao, WANG Yi, ZHANG Hui-Qiong, SUN Si, SUN Yan, WANG Xian-Zhong, ZHANG Jia-Hua. Effect of FSH on the mRNA Expression of Cyclin D1 and Cyclin E1 in the Cultured Immature Boar Sertoli Cell [J]. Scientia Agricultura Sinica, 2013, 46(6): 1237-1246.
[2]	ZHANG Guo-sheng,ZUO Jing,GAN Rui,ZHU Feng-wei,SUN Yan,WANG Xian-zhong,ZHANG Jia-hua. Effect of Combination of FSH and 17β-estradiol on the Expression of Skp2 in Cultured Immature Boar Sertoli Cell [J]. Scientia Agricultura Sinica, 2011, 44(11): 2347-2354 .
[3]	Ayimuguli1,,CAI Yong,CAO Xin,LONG Ling,CHEN Shi-en,YU Si-jiu,CUI Yan1. Different Expression of Estrogen Receptor α and Progesterone Receptor in Yak Uterus During the Estrous Cycle [J]. Scientia Agricultura Sinica, 2011, 44(11): 2355-2363 .