Abstract: 【Objective】To probed into the feasibility of isotopic traceability in beef geographical traceability in China, and found out the function of δ13C and δ15N values in it. 【Method】The δ13C and δ15N values of beef, cattle hair and feeds coming from four regions of Jilin, Guizhou, Ningxia, Hebei were determined using isotope ratio spectrometer, the difference of carbon and nitrogen composition of beef samples from different regions were compared, the correlations of isotope composition between cattle tissues were analyzed, and the changing laws of δ13C and δ15N values of beef were researched combining feeding mode. 【Results】The δ13C values of cattle tissues from different regions had much significant difference, the order was Jilin>Guizhou>Ninxia>Hebei. The δ15N values of cattle tissues also had obvious significant difference between the region of Jilin, Ningxia and the region of Guizhou and Hebei, and the latter two was higher than the former two. The staple feeds from different regions had their own character. In addition, the correlations of δ13C and δ15N values between defatted muscle, crude fat and cattle hair were very significant. 【Conclusion】The δ13C values of cattle tissues could predict the proportion of C4 feeds in their diets, and the δ15N values could discriminate the cattle which feeding with grass and cereal. The identification rates for the cattle geographical traceability using δ13C values were higher than using δ15N values, and the identification rates were improved using both δ13C and δ15N. The δ13C and δ15N values of defatted muscle, crude fat, cattle hair could be used for beef geographical origin assessment, and they provided the consistent information to geographical traceability.