Abstract: 【Objective】This study is to compare the protein composition of the high royal jelly producing bee (A. m. ligustica) with that of carniolians (A. m. carnica) during their worker larval developmental stage.【Method】The experiment was carried out by two-dimensional gel electrophoresis.【Result】 The results showed that significant higher number of total proteins (283) were detected in larvae of high royal jelly producing bees (Jelly bee) than those of carniolian bees (152) on 2-day old larvae. Among them 110 proteins were presented on both of the strains bees larvae, while 173 proteins were specific to larvae of Jelly bees, and 42 proteins were exclusive to carniolian larvae. While on the fourth day, a significant higher number of total proteins (290) were detected in larvae of Jelly bees than those of carniolian bees (240), 163 proteins resolved to both bees larvae, and 127 proteins were specific to Jelly bees and 77 proteins to carniolian bees. Until the sixth day, also a significant higher number of total proteins (236) were detected in larvae of Jelly bees than those of carniolian bees (180), 132 proteins were constantly expressed in two bees larvae, while 104 and 48 proteins are unique to Jelly bees and carniolian larvae, respecitively.【Conclusions】we tentatively concluded that the metabolic rate and gene expression of Jelly bees larvae is more higher than those of carniolian bees based proteins detected as total proteins and proteins specific to each stage of two strains of bee larvae. Proteins constantly expressed on 3 stages of larval development with some significant differences between two bees strains and, proteins unique to each stage expressed differences in term of quality and quantity, indicating that larval development need house keeping and specific proteins to regulate its growth at different development phage, but the expression mold is different between two strains of larval development.