Abstract: PCR amplification of bacterial gyrA and ParC genes indicated that amino acid codons of Ser-83 and Asp-87 were mutation from gyrA and parC genes were not mutation in fluoroquinolone-resistant strains. Conjugational transfer test was performed using donor resistant to tetracycline (TE), ampicillin (AMP), Sulfamethoxazole-trimethoprim ( SXT) and recipient sensitive to TE、AMP、SXT. Result showed that resistance of TE、AMP、SXT could transfer among Escherichia coli and frequency of TE conjugal transfer is 3×10-7. Mixed two groups chicken which inoculated multi-antimicrobial-resistant Escherichia coli (E.loli) and multi-antimicrobial-resistant salmonella (sal) respectively. The multi-antimicrobial-resistant E.coli and sal could be isolated from all chicken after inoculated 3days. But CIP-resistant strains decreased during the time run out and disappeared after inoculated 23days. The result indicated antimicrobial-resistant bacteria can diffuse quickly and unforced eliminated quickly from vivo of host if only the chicken without pressure of antibiotic.