Abstract: Sequencing and analysis of HMW glutenin subunit gene Tibetan Dy10 in a novel subunit combination of Tibetan Dx5*+Tibetan Dy10 in Tibetan wheat landrace YAN Ze-hong, DAI Shou-fen, LIU Deng-cai, WEI Yu-ming, ZHENG You-liang Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan City, Sichuan province, China, 611830 Abstract: [Objective] The objective of this study is focus on elucidating the main difference between HMW glutenin subunit gene Tibetan Dy10 in a novel HMW gluenin subunit combination of Tibetan Dx5*+Tibetan Dy10 in Tibetan wheat landrace and Dy10 in subunit combination of Dx5+Dy10 at molecular sequence level. [Method] The SDS-PAGE analysis, cloning and sequencing method were used. [Result] The result indicated that the molecular sequence of HMW glutenin subunit gene Tibetan Dy10 was very similar to that of Dy10 in HMW glutenin subunit combination Dx5+Dy10. The difference is that one amino acid in each of the two hexpeptide repetitive motifs in Dy10 has been substituted in Tibetan Dy10, respectively. [Conclusion] Thought minor differences were found between Tibetan Dy10 and Dy10, there still have the reason that Tibeatan Dy10 is a Dy10 but not others. Keywords: Tibetan wheat landrace; a novel HMW glutenin subunit combination; y-type HMW glutenin subunit; Sequencing