Abstract: This study made 17 strains of Lentinula edodes，including wild strains and cultivated strains which well-popularized around China as materials, statistically analyzed the ratios of spores from different aspects via mating types analysis and OWE-SOJ technique. Results from this study firstly systematically identified skewed expected distribution of mating-type factors segregation in Lentinula edodes spores has commonly statistical meanings in wild and cultivated strains. Genetic analysis to positive and negative parental-recombined fruiting showed that nuclear type of F1 progeny spores among those strains segregated theoretically distribution mainly depended on combined state of parental dikaryons, predominant spores were those with mating type identical to dikaryotic parent, indicating that the genetic basis of segregation distortion of spores is different from that of protoplast monokaryons in which B factor takes predominant responsibility for that phenomenon, it cooperate A factor with B factor to influence the ratio of spores.

Key words: Mating-type factor, basidiospore, Lentinula edodes, segregation distortion,