Scientia Agricultura Sinica ›› 2006, Vol. 39 ›› Issue (9): 1736-1742 .
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Abstract: 98-160 is a wheat line with excellent salt-tolerance which is controlled by major genes. The expression profile of 98-160 under salt stress for different times was studied using cDNA-AFLP. One hundred and twelve fragments were cloned and sequenced. The tBlastx analysis showed that 62.5% of the sequenced fragments had similarity with known genes in the database, in which 18 cDNA were tightly related to wheat salt tolerance, most of them were transcription factor, transportation related protein, signal transduction related protein and proteins related to other stresses. Some fragments encoded proteins involved in protein-protein interaction.Several full length cDNAs have been obtained based on the above fragments, expression analysis by RT-PCR indicated that they might play important roles in reaction to wheat salt stress.
Key words: wheat salt tolerance cDNA-AFLP
. Isolation of cDNA fragments and genes related to wheat salt-tolerance via cDNA-AFLP[J].Scientia Agricultura Sinica, 2006, 39(9): 1736-1742 .
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