Scientia Agricultura Sinica ›› 2006, Vol. 39 ›› Issue (04): 709-714 .

• PLANT PROTECTION • Previous Articles     Next Articles

Structure and Expression Pattern of Several Putative Cdc42-Interacting Proteins in Magnaporthe grisea

,,,,   

  • Received:2005-12-20 Revised:1900-01-01 Online:2006-04-10 Published:2006-04-10

Abstract: 【Objective】 MgCdc42 (Cdc42 in Magnaporthe grisea), with high homology to ScCdc42 (Cdc42 in Saccharomyces cerevisiae), has been demonstrated to involve in its morphogenesis and infection process. To further dissect the signaling network, the putative MgCdc42-interacting proteins were analyzed. 【Method】 ScCdc42-interacting protein sequences were first used to BLAST against the M. grisea genome database to retrieve their corresponding analogs. Subsequently, conserved domains of these proteins were compared and expression patterns of their encoding genes in different MgCdc42 mutation states were analyzed by semi-quantitative RT-PCR.【Result】All retrieved analogs of ScCdc42-interacting proteins from the M. grisea database have conserved domains as those in S. cerevisiae. Expression of their encoding genes increased in MgCdc42CA mutant, and decreased in MgCdc42KO mutant. However, MgBem1, Chm1 and MgGic1 in MgCdc42DN mutant remained the same expression level as that in the wild type although MgBem4, MgBoi2, MgCdc24, MgGic2, MgRga1 and Mst20 decreased as expected.【Conclusion】 Taking together, we conclude that there may exist a similar Cdc42 signal pathway in M.grisea as in S. cerevisiae and MgCdc42 plays a key role in the pathway.

Key words: Cdc42, Magnaporthe grisea, Guanine nucleotide exchange factor, GTPase-activating protein

[1] LIU Yang-1, YIN Feng-Xia-Yin-Feng-Xia-2, BAI Chun-Ling-Bai-Chun-Ling-2, WEI Zhu-Ying-Wei-Zhu-Ying-2, ZAN Lin-Sen-Zan-Lin-Sen-1, LI Guang-Peng-Li-Guang-Peng-2. Expression and Localization and Effect of Cdc42 Protein During the Maturation of Bovine Oocyte [J]. Scientia Agricultura Sinica, 2014, 47(3): 574-585.
[2]
PENG Xi-xu; HU Yao-jun; TANG Xin-ke; ZHOU Ping-lan; DENG Xiao-bo; WANG Hai-hua
. Isolation and Expression Profiles of Rice WRKY30 Induced by Jasmonic Acid Application and Fungal Pathogen Infection [J]. Scientia Agricultura Sinica, 2011, 44(12): 2454-2461 .
[3] ZHOU Jie,ZHENG Xiang-zi,LAN Lan,LIN Cheng-zeng,LIN Xiong-jie,LU Guo-dong,WANG Zong-hua
. A Preliminary Study of the Putative Glycosyl Hydrolase Family 62 in Magnaporthe grisea
[J]. Scientia Agricultura Sinica, 2009, 42(8): 2754-2762 .
[4]

. Secreted Expression of the Combinant Defensin alfAFP (M. sativa) in Pichia pastoris and Its Antimicrobial Activity Against Rice Pathogens in Vitro
[J]. Scientia Agricultura Sinica, 2009, 42(3): 869-875 .
[5]

. Comparison of Differentiating Capacity of Two Sets of Differential Lines Using Isolates of Magnaporthe grisea Collected from China’s Yunnan Province
[J]. Scientia Agricultura Sinica, 2009, 42(2): 486-491 .
[6] SHEN Ying1, LI Cheng-yun2. Current Research Status and Future Prospects of Genetic Diversity of Magnaporthe grisea Fungus [J]. Scientia Agricultura Sinica, 2007, 40(增刊): 3100-3106.
[7] ,,,,,. Prediction for Secreted Proteins from Magnaporthe grisea Genome [J]. Scientia Agricultura Sinica, 2006, 39(12): 2474-2482 .
[8] ,,,,,,. Virulence Variation in Sexual Progeny and Composition of Avirulence Genes from the Cross CH63 and TH16 of Magnaporthe Grisea [J]. Scientia Agricultura Sinica, 2005, 38(12): 2428-2433 .
[9] ,. Mating Type and Fertility of the Rice Blast Fungus Population Derived from Guangdong Province, China [J]. Scientia Agricultura Sinica, 2005, 38(04): 837-842 .
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!