Abstract: 【Objective】Jujube witches’ broom (JWB) is a destructive disease caused by a phytoplasma，that happened in the twenty-five provinces(cities) of china, brought largely economic loss. 【Method】Jujube witches’ broom and wild jujube witches’ broom were investigated in Binxian，Yanliang，Wugong，Yangling，Jiaxian of Shaanxi, Cangzhou of Hebei, Xinxiang of Henan. The 16SrDNA and tuf gene were cloned by polymerase chain reaction from samples of JWB and wild jujube witches’ broom, and sequenced.【Results】The 1239bp and 845bp DNA fragement were amplified in DNA samples not only from infected jujube but also from infected wild jujube. it is found that 16SrDNA is 1239bp, tuf gene is 845bp. The Homology analysis for sequences of 16SrDNA and tuf gene shows that pathogens from Shaanxi, Hebei, Henan are same, and belong to 16SrⅤ-B group. Because there are five differential nucleotides in 16SrDNA between JWB and wild jujube witches’ broom , and homology rate of nucleotide sequences of tuf gene is 91%, we can estimate that wild jujube witches’ broom is infected by different strain of JWB pathogen. 【Conclusion】This article reported sequences of JWB and wild jujube witches’ broom phytoplasmas firstly, and differentiate and classify phytoplasma of JWB and wild jujube witches’ broom. which can provide theory for research of the molecular nosogenesis of JWB phytoplasma and genetic basis.