Abstract: Abstract: Infectious bursal disease (IBD) ,mediatied by infectious bursal disease virus (IBDV),is one of the causive agents which cause significant losses to the poultry industry. In this research, EcoRⅤ site or PstⅠsite ,as the genetic tags,was introduced separately into the A segment or B segment of the IBDV Gt strain.The full-length genome was flanked by hammerhead ribozyme(HamRz) and hepatitis delta ribozyme(HdvRz) sequence. The full-length genome containing genetic tags flanked by HamRz and HdvRz were arranged downstream of the Beta chincken actin promoter of the vector pCAGG.The recombinant plasmid ，which were infectious clones,were named pCAGGmGtAHRT and pCAGGmGtBHRT.These clones can infection DFⅠcell effectively.The results of RT-PCR,indirect immunofluorescence,electron microscope showed IBDV was rescued successfully. The genome of rescued IBDV have genetic tags.The rescued IBDV could cause CPE on CEF,and the TCID50 of rescued IBDV was similar to Gt.The method of rescuing verus was the basis for further researching IBDV.

Key words: infectious bursal disease virus, the Gt strain, genetic tags, ribozyme, virus rescue