Abstract: Abstract: Asymmetric protoplast fusion beween hypocotyl protoplasts of Brassica oleracea var. botrytis and leaf protoplasts of Ogura type CMS Brassica napus was performed by PEG method. Leaf protoplasts from donor plant were treated with UV light at a dose of 0.0250、0.0750、0.3150、0.4680 and 0.9600 J·cm-2 respectively. 68 plants regenerated in the dose range from 0.0250 to 0.4680 J·cm-2,and none at the dose of 0.9600 J·cm-2. Results of chromosome counts、flow cytometry、isozyme analysis、RAPD and specific amplification of Ogura-CMS-associated mitochondrial gene orf138 revealed that 32 somatic hybrid plants were obtained and the CMS-associated mitochondrial gene orf138 had been introduced into the receptor genotype of Brassica oleracea var.botrytis.

Key words: Brassica oleracea var.botrytis, Asymmetric protoplast fusion, Ogura Cytoplasmic male sterility