斜纹夜蛾对乙基多杀菌素的抗性监测及抗性遗传方式

doi:10.3864/j.issn.0578-1752.2026.18.008

Abstract

Abstract:

【Objective】This study aims to clarify the current resistance status and underlying mechanisms of field populations of Spodoptera litura to spinetoram, and to provide a scientific basis for field resistance monitoring and the development of integrated resistance management (IRM) strategies.【Method】From 2023 to 2024, 23 field populations of S. litura were collected across China. The resistance levels to spinetoram were determined using the leaf-dipping method. The highly resistant Nanning (NN) population and the susceptible strain (SS) were subjected to reciprocal crosses and mass crosses, and the F₁ progeny larvae were bioassayed to analyze the resistance inheritance pattern. The synergistic effects of piperonyl butoxide (PBO), diethyl maleate (DEM), and triphenyl phosphate (TPP) were evaluated, and the activities of carboxylesterase (CarE), glutathione S-transferase (GST), and cytochrome P450 monooxygenase (P450) were measured in both the NN and SS strains.【Result】Field resistance monitoring revealed significant variation in spinetoram resistance among different geographic populations of S. litura. High-level resistance (43.3- to 107.8-fold) was detected in populations from Nanning (Guangxi) and Fuzhou (Fujian) in 2023, and from Sanya (Hainan), Kunming (Yunnan), Weifang (Shandong), and Xuchang (Henan) in 2024. Notably, the resistance ratios for the Nanning (2023) and Sanya (2024) populations reached 106.8- and 107.8-fold, respectively. Moderate resistance levels were observed in the 2023 Ningbo (Zhejiang) and Jingzhou (Hubei) populations, as well as the 2024 Fuzhou (Fujian), Jingzhou (Hubei), Guangzhou (Guangdong), Changsha (Hunan), Nanjing (Jiangsu), Shanghai, and Guilin (Guangxi) populations, with resistance ratios ranging from 10.3- to 31.8-fold. Low resistance levels were found in the 2023 Hangzhou (Zhejiang) population and the 2024 Nanchang (Jiangxi), Hefei (Anhui), Beijing, and Hangzhou (Zhejiang) populations, with resistance ratios between 5.0- and 8.9-fold. Inheritance analysis showed that resistance to spinetoram in the NN population was controlled by an autosomal, incompletely recessive gene. The synergists DEM and TPP exhibited synergistic ratios of 1.8 and 1.5, respectively, suppressing resistance in the NN population. Moreover, the activities of GST and CarE in the NN population were 4.1- and 3.5-fold higher than those in the susceptible strain, respectively.【Conclusion】Resistance to spinetoram has developed in S. litura populations across multiple regions. Enhanced activities of GST and CarE play important roles in the resistance of S. litura to spinetoram.

Key words: Spodoptera litura, spinetoram, resistance monitoring, incompletely recessive inheritance, synergism, resistance management

LI Hao, GAO XinJu, LU Peng, ZHAN QianYuan, WANG Ran, SU Qi, QU Cheng. Resistance Monitoring and Inheritance of Resistance to Spinetoram in Spodoptera litura[J].Scientia Agricultura Sinica, 2026, 59(18): 4048-4055.

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References 29

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种群Population	LC₅₀ (95%置信限CL) (mg·L^-1)	斜率±标准误Slope±SE	卡方值（自由度）χ²(df)	抗性倍数Resistance ratio
SS	0.362 (0.204-0.515)	1.403±0.253	1.089 (3)	—
NB	9.824 (7.740-12.444)	1.964±0.258	0.556 (3)	27.1
HZ	3.204 (2.422-4.146)	1.727±0.249	1.371 (3)	8.9
FZ	18.994 (14.296-25.129)	1.611±0.242	2.228 (3)	52.5
JZ	6.095 (4.900-7.594)	2.173±0.272	0.578 (3)	16.8
NN	38.674 (29.130-53.268)	1.537±0.240	0.162 (3)	106.8
BJ	1.022 (0.817-1.281)	2.099±0.268	0.861 (3)	2.8
YZ	1.522 (1.191-2.026)	1.837±0.259	0.762 (3)	4.2
NC	1.416 (1.036-2.070)	1.370±0.233	0.130 (3)	3.9

种群Population	LC₅₀ (95%置信限CL) (mg·L^-1)	斜率±标准误Slope±SE	卡方值（自由度）χ²(df)	抗性倍数Resistance ratio
SS	0.321 (0.222-0.413)	2.269±0.358	1.314 (3)	—
HZ	1.609 (1.239-2.045)	1.880±0.254	1.661 (3)	5.0
FZ	8.779 (6.381-11.034)	2.404±0.368	0.854 (3)	27.3
JZ	7.293 (5.622-9.200)	1.957±0.261	0.944 (3)	22.7
BJ	1.967 (1.401-3.078)	1.252±0.231	1.163 (3)	6.1
NC	2.733 (2.116-3.558)	1.770±0.249	0.621 (3)	8.5
SY	34.613 (27.430-42.745)	2.230±0.284	0.611 (3)	107.8
GZ	10.192 (8.127-12.801)	2.074±0.268	2.914 (3)	31.8
CS	3.321 (2.469-4.275)	1.766±0.256	2.309 (3)	10.3
NJ	4.214 (3.344-5.245)	2.122±0.271	1.069 (3)	13.1
HF	2.162 (1.644-2.682)	2.371±0.335	1.217 (3)	6.7
SH	5.978 (4.291-7.623)	2.105±0.313	1.441 (3)	18.6
WF	17.261 (13.547-21.451)	2.141±0.278	2.053 (3)	53.8
GL	5.269 (3.923-6.600)	2.275±0.326	2.638 (3)	16.4
KM	21.339 (16.842-27.147)	1.953±0.260	1.714 (3)	66.5
XC	13.909 (10.764-18.610)	1.729±0.251	2.191 (3)	43.3

种群 Population	检测数 Number of insects	LC₅₀（95%置信限CL） (mg·L^-1)	斜率±标准误 Slope±SE	抗性倍数 Resistance ratio (RR^a)	显性度分析 Degree of dominance (D^b)
SS	600	0.359 (0.18-0.53)	0.646±0.121	—	—
NN	600	38.674 (29.130-53.268)	1.573±0.240	107.7	—
正交F₁	600	3.673 (2.879-4.667)	1.691±0.174	10.2	-0.006
反交F₁′	600	3.131 (2.518-3.870)	2.018±0.201	8.7	-0.070
混交F₁ pooled	600	3.485 (2.700-4.475)	1.592±0.168	9.7	—

品系 Strain	处理 Treatment	LC₅₀ (95%置信限CL) (mg·L^-1)	斜率±标准误 Slope±SE	增效比 Synergistic ratio
SS	乙基多杀菌素Spinetoram	0.388 (0.290-0.504)	1.695±0.247	—
	乙基多杀菌素+胡椒基丁醚Spinetoram+PBO	0.445 (0.325-0.575)	1.756±0.259	0.9
	乙基多杀菌素+马来酸二乙酯Spinetoram+DEM	0.417 (0.282-0.550)	1.775±0.275	0.9
	乙基多杀菌素+磷酸三苯酯Spinetoram+TPP	0.351 (0.281-0.436)	2.174±0.271	1.1
NN	乙基多杀菌素Spinetoram	42.830 (32.325-55.777)	1.686±0.246	—
	乙基多杀菌素+胡椒基丁醚Spinetoram+PBO	43.340 (28.321-58.281)	1.601±0.261	1.0
	乙基多杀菌素+马来酸二乙酯Spinetoram+DEM	24.061 (18.756-30.737)	1.874±0.255	1.8
	乙基多杀菌素+磷酸三苯酯Spinetoram+TPP	29.256 (22.868-38.867)	1.819±0.257	1.5

种群 Population	谷胱甘肽S-转移酶活性 Activity of GST (U·mg^-1 protein)	羧酸酯酶活性 Activity of CarE (U·mg^-1 protein)	细胞色素P450酶活性 Activity of CYP450 (U·L^-1 protein)
SS	35.53±0.55b	4.73±0.58b	4.82±0.12b
NN	143.97±2.03a	16.71±1.10a	7.77±0.13a

Resistance Monitoring and Inheritance of Resistance to Spinetoram in Spodoptera litura

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