Scientia Agricultura Sinica ›› 2016, Vol. 49 ›› Issue (14): 2763-2771.doi: 10.3864/j.issn.0578-1752.2016.14.010

• HORTICULTURE • Previous Articles     Next Articles

The Role of CBF Cold Response Pathway Gene in Heat Treatment-Induced Chilling Tolerance in Banana Fruits

WANG Hai-bo1, LI Lu2, SU Xin-guo1, ZHANG Zhao-qi3, PANG Xue-qun2   

  1. 1Guangdong Food and Drug Vocational College, Guangzhou 510520
    2College of Biotechnology, South China Agricultural University, Guangzhou 510642
    3College of Horticulture, South China Agricultural University, Guangzhou 510642
  • Received:2016-03-14 Online:2016-07-16 Published:2016-07-16

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Abstract: 【Objective】The objective of this study is to investigate the role of C-repeat binding transcription factor (CBF) cold-resistance pathway in heat treatment -induced chilling tolerance in banana fruits and to provide reference for the study of the signal transduction of CBF cold response pathway in banana fruits.【Method】The sequences of 7 genes related to CBF cold response pathway were selected from the banana genome database (http://banana-genome-hub.southgreen.fr/), and then specific primers were designed respectively. Analysis of these 7 genes’ expression patterns of the heat-induced chilling tolerance in banana fruits was conducted using quantitative real-time PCR.【Result】The expression of MaICE gene of banana fruit increased rapidly and reached the maximum level at 7℃ for 1 hour. The expression of DREB (MaDREB1D, MaDREB1E, MaDREB1G and MaDREB3) genes displayed a peak at 7℃ for 1 hour. The expression of MaCOR413 gene had a peak at 7℃ for 4 hours. These results indicated that the CBF cold response pathway (ICE-CBF-COR pathway) exist in banana fruit during cold storage at 7℃. The expression of 7 gene related to CBF cold response pathway reached the maximum levels at 0.5 hour after heat treatment (52℃ 3 min). Furthermore, when the heat treated banana fruits storage at 7℃ for 5 days, the expressions of MaDREB1D, MaDREB1E, MaDREB2C, MaDREB3 and MaCOR413 genes were higher than the non-heated control.【Conclusion】 MaICE, DREB, and MaCOR413 increased in turn when banana fruits stored at 7℃. The CBF cold response pathway exist in banana fruits during cold storage at 7℃. Enhancement of gene expression related to CBF cold response pathway may be involved in chilling tolerance induced by heat treatment in banana fruits.

Key words: CBF/DREB, banana fruit, heat treatment, chilling tolerance

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