超级稻宜优673遗传构成与籼粳属性分析

doi:10.3864/j.issn.0578-1752.2013.10.001

Abstract

Abstract: 【Objective】 This study was conducted to determine the allelic constitution and subspecies attribution of the super hybrid rice Yiyou 673, so as to facilitate the utilization of beneficial alleles carried by Yiyou 673, and to provide information for establishing an appropriate indica-japonica proportion in rice breeding. 【Method】 Three major restorer lines of the three-line hybrid rice, including the founder parent Minghui 63, its derivative Minghui 86, and Fuhui 673 which is derived from Minghui 86, as well as cytoplasmic male sterile line Yixiang A, and Yiyou 673 which is developed by crossing Fuhui 673 to Yixiang A, were assayed with 17 markers for 13 genes of agronomically importance and 15 SSR markers for indica-japonica differentiation. 【Result】 Polymorphism was detected in eight of the gene marker regions, including two genes for heading date and yield traits, two key genes for grain quality, and four genes for blast resistance. At Wx locus which is responsible for amylose content and gel consistency of the milled rice, a high-quality allele was transmitted from Minghui 63 to Fuhui 673 through Minghui 86. At ALK locus controlling gelatinization temperature and two loci for blast resistance, Pib and Pita, the male and female parents of the F1 hybrid rice Yiyou 673 showed allelic complementation for high grain quality and resistance to blast. In addition, it was shown that Minghui 63, Minghui 86 and Fuhui 673 are all indica-inclined, but the japonica component of 40.0% in Minghui 86 and Fuhui 673 is higher than the value of 33.3% in Minghui 63.【Conclusion】 Beneficial alleles derived from Minghui 63 and other parental lines were pyramided in Fuhui 673, which were combined with those carried by Yixiang A to establish a favorable allelic complementation in Yiyou 673.

Key words: super hybrid rice , Yiyou 673 , subspecies differentiation , genetic composition

HUANG Ting-Xu, ZHU Yu-Jun, FAN Ye-Yang, YOU Qing-Ru, ZHOU Peng, YANG Dong, XIE Hua-An, ZHUANG Jie-Yun. DNA-Marker Based Analysis of the Allelic Constitution and Subspecies Attribution of Super Hybrid Rice Yiyou 673[J].Scientia Agricultura Sinica, 2013, 46(10): 1965-1973.

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DNA-Marker Based Analysis of the Allelic Constitution and Subspecies Attribution of Super Hybrid Rice Yiyou 673

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