Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (23): 4883-4890.doi: 10.3864/j.issn.0578-1752.2012.23.015

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Cloning and mRNA Expression of Smad4 Gene in Ovaries of Erhualian Pig

 ZHAO  Yong-Xiang, LIU  Ji-Ying, PAN  Zeng-Xiang, ZHANG  Jiu-Feng, YAO  Yong, ZHOU  Ji-Long, XIE  Zhuang, XU  Yin-Xue, LIU  Hong-Lin, LI  Qi-Fa   

  1. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095
  • Received:2012-04-01 Online:2012-12-01 Published:2012-04-28

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Abstract: 【Objective】To investigate The sequence features and tissue distribution patterns of Smad4 gene in Erhualian pigs were investigated, and the Smad4 mRNA expression differences in ovarian tissue between Erhualian and commercial cross pigs were analyzed. 【Method】The cDNA sequence of Smad4 gene was obtained by cloning and sequencing techniques in Erhualian pigs, the gene sequence characteristics, physical and chemical properties and the 3D structure of Smad4 protein were analyzed by using bioinformatics methods. The tissue distribution patterns of Smad4 gene were detected by RT-PCR, and then the mRNA expression levels were detected in ovarian tissue of Erhualian and commercial cross pigs by real-time PCR.【Result】The full length of Smad4 gene coding sequence was 1 659 bp in Erhualian pigs, encoding for a protein with 552 amino acid residues. The coding amino acid sequence was highly conservative with other mammals (more than 98% identity). Same as other mammals, Smad4 protein in Erhualian pigs also had three typical structural domains (MH1, SAD and MH2). RT-PCR assays revealed that Smad4 gene of Erhualian pigs widely expressed in all tissues. In addition, Smad4 mRNA levels in Erhualian pig ovary was significantly higher than that in commercial pigs (P＜0.01). 【Conclusion】 The Smad4 gene in Erhualian pigs may be own the same function as other mammals and exert probably associated with the pig prolificacy.

Key words: Erhualian pig , Smad4 , ovary tissue , mRNA expression level

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