Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (1): 52-66.doi: 10.3864/j.issn.0578-1752.2012.01.007

• PLANT PROTECTION • Previous Articles     Next Articles

SSR Marker Development and Analysis of Genetic Diversity of Fusarium verticillioides Isolated from Maize in China

 REN  Xu, ZHU  Zhen-Dong, LI  Hong-Jie, DUAN  Can-Xing, WANG  Xiao-Ming   

  1. 1.中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大工程,北京 100081
  • Received:2011-04-06 Online:2012-01-01 Published:2011-05-12

Abstract: 【Objective】 The objective of this study is to analyze genetic diversity of Fusarium verticillioides and to develop SSR markers for this fungus. 【Method】 Fastpcr was used for searching SSRs in genomic sequence of F. verticillioides. The sequences including suitable SSRs were selected to design primers using Primer5.0. The NTSYS and Popgene programs were used to analyze genetic diversity of maize isolates of F. verticillioides. 【Result】 Among the 158 primer pairs designed, 109 pairs (69.0%) were able to amplify 2 or more alleles against discretionary F. verticillioides isolates originating from single conidium. Of them 55 pairs (34.8%) amplified 3 or more alleles. In 11 chromosomes assembled 22 primer pairs, two pairs for one chromosome, were selected for genetic diversity analysis of 66 isolates of F. verticillioides from maize. In 22 SSR loci, a total of 125 alleles (2 to 11 alleles amplyfied by per primer pair) were detected with an average of 5.68 alleles. The Nei’s gene diversity, ranged from 0.1139 to 0.8687 with an average mean of 0.6199, indicated high genetic diversity in F. verticillioides isolates from various geographic regions.【Conclusion】Based on genome information of F. verticillioides, 109 SSR markers were developed and showed high polymorphism and high diversity among F. verticillioides isolates. By amplyfying with 22 SSR primer pairs, 66 isolates of F. verticillioides from maize were seperated into 3 groups on 0.3 coefficient. The relationship between genetic diversity and geographical distribution did not show correlation in isolates of F. verticillioides from maize in China.

Key words: Fusariumverticillioides, simplesequancerepeat(SSR), pathogenisolatefrommaize, geneticdiversity

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