北京地区不同桃蚜种群的抗药性研究

doi:10.3864/j.issn.0578-1752.2011.21.007

Abstract

Abstract: 【Objective】 The objective of this study is to survey the resistance level of peach aphid Myzus persicae (Sulzer), one of the most serious pest in Beijing area. 【Method】 Bioassay of five frequently used pesticides to six field populations and a sensitive population was conducted, the kdr resistance was detected by bi-PASA PCR method, and the esterase and acetylcholinesterase activities of this pest were tested. 【Result】 The results show that both the six field populations, in Tongzhou, Shunyi, Daxing, Haidian, Yanqing and Mentougou and the sensitive population developed resistance to beta-cypermethrin. The proportion of resistant homozygote was higher than 90% in all populations except for Yanqing and Mentougou. Mentougou, Tongzhou and Yanqing populations developed resistance to chlorpyrifos and imidacloprid with resistance ratio of 2.05-4.24 and 32.03-41.27, respectively. All of the field populations were sensitive to abamectin and emamectin benzoate (the resistance ratio was 0.19-1.44), except for the Daxing population (the resistance ratio was 3.45 and 2.82, respectively) and Haidian population to abamectin (the resistance ratio was 2.66). There was a significant positive correlation between the esterase activity and resistance ratio to chlorpyrifos and imidacloprid, while there was a negative correlation between acetylcholinesterase activity and resistance ratio of chlorpyrifos and imidacloprid. 【Conclusion】 The peach aphid in Beijing area developed resistance to nearly all frequently used pesticides tested in this study. The beta-cypermethrin should not be used, and the chlorpyrifos should be avoided, and abamectin, emamectin benzoate and imidacloprid could be used alternatively.

Key words: Myzus persicae (Sulzer), resistance to pesticide, bioassay, enzymatic activity, knockdown resistance

GONG Ya-Jun, WANG Ze-Hua, SHI Bao-Cai, KANG Zong-Jiang, ZHU Liang, GUO Xiao-Jun, LIU Jian-Hua, WEI Shu-Jun. Resistance Status of Myzus persicae (Sulzer) (Hemiptera: Aphididae) Populations to Pesticide in Beijing[J].Scientia Agricultura Sinica, 2011, 44(21): 4385-4394.

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Resistance Status of Myzus persicae (Sulzer) (Hemiptera: Aphididae) Populations to Pesticide in Beijing

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