壳聚糖为递送载体的鸭肠炎病毒gC基因疫苗诱导Balb/c小鼠

doi:10.3864/j.issn.0578-1752.2011.16.019

Abstract

Abstract: 【Objective】 The objective of the experiment is to investigate the influence of chitosan on the immunoreaction induced duck enteritis virus gC genetic vaccine. 【Method】 Balb/c mice were immunized with 50µg per dose of DEV gC genetic vaccine with chitosan as deliver carrier by a single intramuscular injection and oral. Naked plasmid groups of different immunizing doses by intramscular injection, live attenuated vaccine group and physiological saline group were applied as control groups. Cellular immunity, humoral immunity and mucosal immunity were detected, respectively. 【Result】 Compared with DEV gC genetic vaccine with chitosan as deliver carrier by oral and 50 µg of naked plasmid group by intramuscular injection, DEV gC genetic vaccine with chitosan as deliver carrier by intramuscular injection induced stronger cellular and humoral immunity. This vaccine induced similar cellular immune response compared with live attenuated vaccine that induced stronger humoral immune responses. Mucosal immunity was induced only by oral DEV gC genetic vaccine with chitosan as deliver carrier. 【Conclusion】 Chitosan had certain adjuvant’s ability. It has provided a new strategy to obstain more effective DEV gC genetic vaccine. Many factors affecting the immunity efficiency of vaccine need to be optimiazed further for application of this vaccine generally .

Key words: chitosan, DEV gC gene, genetic vaccine, cellular immunity, humoral immunity, mucosal immunity

JIANG Jin-Feng, CHENG An-Chun, WANG Ming-Shu, LU Li-Ting, ZHU De-Kang, JIA Ren-Yong, CHEN Xiao-Yue. The Immunoreaction in Balb/c Mice Immunized with Duck Enteritis Virus gC Genetic Vaccine with Chitosan as Deliver Carrier[J].Scientia Agricultura Sinica, 2011, 44(16): 3454-3462.

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The Immunoreaction in Balb/c Mice Immunized with Duck Enteritis Virus gC Genetic Vaccine with Chitosan as Deliver Carrier

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