Abstract:

【Objective】This study was carried out to obtain high-yielding β-1,3-1,4-glucanases gene engineering strain from Paenibacillus polymyxa CP7 for the research of anti-fungi components, and provide a theoretical basis and application in agricultural production for β-1,3-1,4-glucanases.【Method】After cloning and expression of the β-1,3-1,4-glucanases gene in Escherichia. coli strain BL-21, the inhibitory effects of recombinant protein on the tested fungus were detected, and the effect of recombinant β-1,3-1,4-glucanase on animal feed digestibility in vitro using wheat as raw material of feed was studied.【Result】The bioassay results reveal that the recombinant β-1,3-1,4-glucanase has strong inhibitory effects on the testing fungus with different activities between them, and the inhibitory ratio reached up to 40% above. Compared with the control, the viscosity of wheat sample treated with the recombinant protein was reduced by 4.69% (P>0.05). 【Conclusion】 β-1,3-1,4-glucanase is or one of the effective components of active compounds, restrains the fungus of Paenibacillus polymyxa CP7 crude extracts, which suggest β-1,3-1,4- glucanase can be used as a new kind of agricultural chemicals for development, meanwhile, it is also a prosperous feedstuff additive.

Key words: Paenibacillus polymyxa CP7, glucanase, gene cloning and expression, wheat feed