Abstract:

【Objective】 The present paper dealt with isolation and identification of the endophytic bacteria from Musa plants as well as the characteristics of their PLFA biomarkers. 【Method】 Healthy Musa plants were collected from Zhangzhou areas in Fujian province, China. The endophytic bacteria inside the plants were isolated by streak plate method and identified by using fatty-acid identification technology. 【Result】 The results showed that 14 species of endophytic bacteria were identified by means of microbe identification system. 9 microbial species belonged to Musa itinerans, 7 species belonged to Musa AAA cavendish var.Williams and 3 species belonged to Musa basjoo. There were 35 PLFA biomarkers detected in 14 species of endophytic bacteria, in which based on the distribution frequency in 4 types of bacteria were clustered, e.g. (1) high frequency related to characteristics of microbe in general and the PFLA were detected in 10-14 times in 14 strains of bacteria, (2) middle frequency related to bacteria in general and the PFLA were detected in 4-6 times in 14 strains of bacteria, (3) low frequency related bacterial genus and the PFLA were detected in 2-3 times in 14 strains of bacteria, (4) tiny frequency related to species and the PFLA only in a bacteria. According to the PLFA biomarkers and the cluster analysis method, the 14 species were classified into 3 groups based on the PLFA biomarkers. The diversity and richness, uniformity and dominance of the concept index ecology were used to analyze the relationship between the fatty acid biomarker gross and ecological parameters. The results showed that the richness of 16:0,18:1 W7C, 14:0, 12:0, 18:0, and 17:0 CYCLO of PLFA biomarkers was relatively high. The higher fatty acids biological response value, the lower the sum uniformity (J), the dominance of the Simpson index (D), Shannon-Wiener (H1), Brillouin (H2), Mcintosh (H3) diversity index were relatively high. 【Conclusion】 There are significant polymorphism among community structure of endophytic bacteria and their PLFA biomarkers in Musa plants.

Key words: Musa plants, endophytic bacteria, PLFA biomarkers