Abstract:

【Objective】 The effect of nano coating on the main enzymes activities, components of cell wall and economic characters of tobacco leaves was studied during the flue-curing in order to provide a theoretical basis for optimization of curing technology and its application in large areas. 【Method】 Differences in physiological and biochemical reactions of cell wall and economic characters of upper leaves between the treatment of nano coating and control were studied by using the electric-heated flue-curing barn designed and made by Henan Agricultural University. 【Result】 The peaks of the pectin methylesterase, polygalacturonase and cellulase activities appeared at 42℃, 48℃ and 38℃, respectively. The peaks of the PG and cellulase activities appeared earlier than that of the control, but the enzymes activities of cell wall were lower than the control in the later curing. The content of cellulose in treatment was always obviously higher than the control and the cured sample of treatment was 12 mg?g-1 higher than the control. The content of soluble pectin, protopectin and total pectin in treatment was also obviously higher than the control when the temperature was higher than 42℃. The highest content of total pectin in treatment was 6.34% at 42℃. High class leaf and total output value in treatment increased by 7.33% and 16.56% respectively as compared with the control and the differences between them were all highly significant. At the same time the weight per leaf was 0.71 g higher than the control and the difference was significant.【Conclusion】Nano coating had a great effect on enzymes activities and components of cell wall and economic characters of upper leaves. The result showed that it could improve economic benefit. It has provided a reference for further application of curing technology on the basis of the functions of nano coating and the change rules of physiological and biochemical reactions of cells of tobacco leaves with nano coating barn treatment.

Key words: nano coating, flue-cured tobacco, enzymes activities of cell wall, cell wall components, economic characters