Abstract: Abstract: By serological ELISA, and TBE electrophoresis of viral RNAs，the pathogen in chlorosis symptom on the seedlings of Capsicum frutescens from Hangzhou was Cucumber mosaic virus(CMV-Phy). When inoculated with the CMV-Phy viroin, Nicotiana glutinosa expressed chlorosis symptom. The complete genome of CMV-Phy RNA1，RNA2 and RNA3 was obtained in a reaction，by touch-up RT-PCR. Full length of RNA1, RNA2 and RNA was 33356 nt, 3048 nt and 2220nt, respectively. Sequences aliment suggested that CMV-Phy belonged to SubgroupⅠbut consisted of SubgroupⅠA RNA1, RNA2 and SubgroupⅠB RNA3. In vitro transcription efficiency improved but transcripts RNA was no activity when guanosine added to the 5′ end of CMV-Phy genomic RNA1, RNA2 and RNA3. Biological assays of CMV-Phy infectious clone showed that the symptom induced by CMV-Phy infectious clone was the same as that induced by its virus particle.