Abstract: 【Objective】Mite（varroa jacobsoni）is one of the most serious parasite threat to the honey bee (Apis mellifera) reared in china. We mainly use the drug to control and kill the mite for these years, but the honey products may be contaminated and the mite is becoming drug-resistant. Our main idea is to research the possibility to rear the mite-resistant honeybee by the nutritional crossbreed. 【Method】Breed the larvae (Apis mellifera ligustica) with the royal jelly of Apis carana carana, and then measure the morphological index of the worker generation, genotypic frequency and gene frequency of the MHCⅡ, genetic resemblance and mite resistance..【Result】Compared with the parent workers the results showed: proboscis length, anterior wing area, the total length of 3rd and 4th dorsal plate of abdominal, the length of 4th dorsal plate tuberculum, the area of 6th abdominal segment, the area of wax mirrors are significant different, but the differences of the brachium index, dactylus index and wing claw are not significant. And there are some mutation in the genotypic frequency and gene frequency of the MHCⅡ. The mite resistance of the nutritional crossbreed worker are significantly higher.【Conclusion】We can change the morphological, physical and biochemical character, genetic resemblance and the mite resistant ability of the worker generation by the nutritional crossbreed. The nutritional crossbreed can be a new way to breed the honeybee.

Key words: honeybees, Varroa destructor, nutritional crossbreed