关键词: 小豆, SSR, 遗传多样性

Abstract:

【Objective】 Adzuki bean originated from China, and there are abundant germplasm in China. Evaluation of the genetic diversity can accelerate the application of them in breeding program. 【Method】 In this study, 51 pairs of SSR primers were used to assess the genetic diversity and structure among 137 Chinese and eight alien adzuki bean germplasm. 【Results】 A total of 222 alleles were detected and the alleles with frequency lower than 5% accounted for about 35.9%. The number of alleles changed from 2 to 13 with an average of 4.35 per locus. The PIC value varied from 0.014 to 0.838, and the mean PIC value for SSRs on each linkage group was 0.440. The similarity coefficient between accessions varied from 0.227 to 0.951 and averaged at 0.487. Adzuki bean from Hubei and Shaanxi provinces had much higher genetic variations, and their genetic backgrounds are much different from that of other provinces. Cluster analysis divided the 145 accessions into five groups, which is much agreeable with that obtained in analysis by using STRUCTURE package, and the accessions within five groups almost related with their origins of geographical distribution. 【Conclusion】 There are abundant genetic variations in Chinese adzuki bean. The genetic differentiation among adzuki bean from different origins is important in dividing ecological region for Chinese adzuki bean.

Key words: adzuki bean, SSR, genetic diversity