关键词: 高热症, 猪链球菌, PCR, 分型, 毒力因子, 病理学

Abstract:

【Objective】 The objective of this experiment is to study on serotype, distribution of virulence gene and pathology of 11 strains of Streptococcus, isolated from heart blood, liver, lymph node, kidney, lung and other tissues of swine from different areas in Hubei province, China. 【Method】 ATB aotu-biochemical reaction test system, PCR typing, PCR test of virulence factor genes and animal test assays were used. 【Result】 PCR typing assay indicated that 11 strains were Streptococcus suis including 1 serotype 7 strains, 2 serotype 9 strains, 8 other serotype strains, no serotype 1 strain or serotype 2 strain. Result of the genotypes of their mrp, epf, sly, orf2, fbps, gdh and mouse virulence test showed that the genotypes of high virulence streptococcus were epf+orf2+ gdh+ fpbs+ strains without mrp+ sly+. Pathobiology showed that acute septicaemia, extensive blooding, lung fever, nephritis, congestion-dropsy in spleen and lymphatic abscess in all over the body, espciouly in the acute death. 【Conclusion】 Serotype 7 and serotype 9 existed in Hubei Province, streptococcus suis has some pathogenicity to ardent fever of swine.

Key words: ardent fever, streptococcus, PCR, typing, virulence factor, pathology