关键词: 苦荞, AFLP, 种质资源, 遗传多样性

Abstract:

【Objective】 The genetic diversity of tartary buckwheat accessions was analyzed at molecular level, and the helpful information for breeding and germplasm evaluation was obtained. 【Method】 The genetic diversity of 165 accessions of tartary buckwheat from 14 different geographical regions was analyzed using 20 informative primer pairs of AFLP markers. 【Result】 Totally 938 loci were detected among which 314 (33.48%) were polymorphic. The number of amplified fragments and polymorphic fragments per primer combination were 46.9 and 15.7, respectively. Shannon’s information index of different geographical groups was 0.1093-0.2661. The group from Sichuan possessed the highest level of genetic diversity, followed by those from Qinghai, Yunnan and Gansu/Ningxia. The group from Hunan was the lowest in genetic diversity. Five cluster groups were identified based on the dendrogram of pairwise Nei’s genetic identity. The clustering results revealed that the genetic diversity of accessions of tartary buckwheat closely related to their origins. Five types of population structure within 165 tartary buckwheat accessions were inferred by structure analysis, which also correlated to their geographic origins. The population structure of accessions from Yunnan and Sichuan provinces were the most complicated and multiplex. 【Conclusion】 The result showed that the genetic relationship and diversity of tartary buckwheat populations correlated to their geographic distribution to a certain extent.

Key words: tartary buckwheat, AFLP, genetic resources, genetic diversity