中国农业科学 ›› 2007, Vol. 40 ›› Issue (10): 2331-2335 .

• 畜牧·兽医·资源昆虫 • 上一篇    下一篇

钙蛋白酶抑制蛋白(CAST)基因在鸡不同组织和品种中的表达差异

刘安芳,朱 庆,刘益平,张增荣   

  1. 四川农业大学动物科技学院
  • 收稿日期:2006-06-15 修回日期:1900-01-01 出版日期:2007-10-10 发布日期:2007-10-10
  • 通讯作者: 朱 庆

Expression Diversity of Calpastatin Gene among Chicken Different Breeds and Tissues

  1. 四川农业大学动物科技学院
  • Received:2006-06-15 Revised:1900-01-01 Online:2007-10-10 Published:2007-10-10

摘要: 【目的】了解鸡钙蛋白酶抑制蛋白(calpastatin,CAST)基因的组织特异性表达情况和在不同鸡品种间的表达差异。【方法】利用半定量RT-PCR方法研究CAST基因在鸡不同组织和品种中的表达差异。【结果】CAST基因表达于鸡的各个不同组织中,在胸肌中的表达量最多并显著高于其它组织(P<0.05);不同鸡品种胸肌组织中CAST基因表达量差异显著(P<0.05),艾维茵肉鸡胸肌组织中CAST基因的表达量显著高于其它几个品种(P<0.05),优质肉鸡品种间差异不显著(P>0.05),但显著高于蛋鸡(P<0.05)。【结论】结果表明钙蛋白酶抑制蛋白是一种在鸡体内普遍存在的蛋白,CAST基因在鸡不同组织和品种中的表达存在显著差异。

关键词: CAST, 半定量RT-PCR, 基因表达, 鸡, 组织和品种

Abstract: Calpastatin(CAST) gene expression diversity among chicken various breeds and tissues was investigated by using semiquantitative RT-PCR.To collect the tissue samples ,20chickens of 5 breeds were slaughtered.The results indicated that CAST gene were expressed in chicken various kinds tissue. The expression in chest muscle was more significant than in other organiz (P < 0.05).moreover,it was identified that CAST gene expression in Avian Broilers was higher than in Vans Nera egg chicken and adequate quality broilers muscles(P <0.05).