关键词: 鸡, 鹌鹑, 杂交早期胚胎, bcl-2, P53, 表达

Abstract: Abstract：【OBJECTIVE】To investigate the influences of apoptotic factors bcl-2、P53 on early embryos of generic hybrids;【METHOD】we acquired the cross bred eggs of chicken(♂)-quail(♀)with artificial insemination,hatched the eggs in one batch according to the standard condition of chicken,collected the early living-embryos of 2.75,3.0,3.25,3.50,3.75,4.0,4.25,4.5,4.75 and 5.0 d at random.Adopted the method of RT- PCR,used multi-ply PCR to identify the embryo sex with primers of Wpkci and β-actin,then selected four embryos of female and male respectively of each period,taken β-actin for internal standard to determine the relative quantity of bcl-2、P53 mRNA of embryos;【RESULTS】1)The bcl-2 mRNA expression of 2.75～4.75 d of male embryos maintained lower level,declined on 4.0 d,then reached the initial level,rose distinctly on 5.0 d(P<0.01)and reached the peak;the bcl-2 mRNA expression of 2.75～4.5 d of male embryos maintained lower level,rose distinctly on 4.75 d(P<0.01),then maintained higher level and reached the peak on 5.0 d;the female bcl-2 mRNA expression of 4.75 d were higher distinctly than male of the same days(P<0.05); 2)The patterns of P53 mRNA expression of male and female embryos were basically consistent.They were higher on 2.75 d,declined on 3.0 d(P<0.05)and reached the lowest level,declined distinctly in male,but the whole differences weren’t significant in female,then reached and maintained the initial level.Comparing the expression between different sex, the differences weren't significant.【CONCLUSION】We found two abnormal points in gene expresstional periods in female(3.0 and 4.75 d),but one point in male(3.0 d).The disorder regulated by key factors of apoptosis may induce embryo death.This conclusion should be confirmed further after the research of bcl-2、P53 expression of chicken and quail's embryos of the same period.

Key words: Chicken, Quail, early embryo of hybrids, bcl-2, P53, Expression