迟播对油菜产量、品质、光合源演替及载荷特征的影响

doi:10.3864/j.issn.0578-1752.2024.23.008

摘要/Abstract

摘要：

【目的】揭示迟播对油菜光合源（叶、角果壳）演替、源载荷及产量品质的调控机制。【方法】于2022—2023年在江苏盐城大丰和南京试点各设裂区试验，主因素设6个播期：10月17日（SD1）、10月23日（SD2）、10月29日（SD3）、11月4日（SD4）、11月10日（SD5）和11月16日（SD6），副因素设常规种甘蓝型油菜南农油4号（抗寒性强）和浙油51（抗寒性中等）2个品种，研究不同播期对油菜叶面积指数（LAI）、角果壳面积指数（PAI）动态变化特征和演替特征，以及源载荷和油菜籽产量品质的影响。【结果】（1）播期越迟，油菜冬前苗龄越小，越冬存活率越低，长江下游油菜最迟安全播期为10月底，冬前苗龄5叶以上。播期推迟至11月，油菜叶龄下降至1.2—3.6叶，平均越冬存活率低于30%。南农油4号11月播种越冬率平均为30.9%，较浙油51高14.5%。（2）最迟安全播期内，随着播期推迟，油菜产量显著下降，单株角果数、千粒重的下降是造成产量下降的最直接因素；对油菜籽脂肪含量和蛋白质含量影响不显著。随着播期的推迟，SD2、SD3较SD1，产量分别下降9.6%、29.0%，单株角果数分别下降6.1%、23.9%，千粒重分别下降4.4%、6.5%。南农油4号籽粒脂肪、蛋白质含量平均为42.9%和25.0%，浙油51分别为47.9%和22.7%。（3）油菜最大LAI、PAI随播期推迟显著下降，LAI达峰值前的缓慢增长速率、达峰值后的快速下降速率皆随播期的推迟而下降；PAI快速增长速率随播期呈减缓趋势；播期越迟，LAI、PAI演替点越早（即有效积温少）和越低（即演替点LAI和PAI都比较低）。南农油4号LAI、PAI动态变化特征值、演替特征值普遍优于浙油51。（4）随播期推迟，叶面积下降幅度快于籽粒产量和角果壳面积下降幅度，造成叶源载荷逐渐增加，壳源载荷逐渐下降。叶源不足和籽粒库不足，是迟播减产的主要原因。南农油4号叶和壳载荷均高于浙油51。【结论】长江下游油菜最迟安全播期内，宜选用叶和角果壳光合源演替点适宜、源载荷较高的耐寒性强、高产高油品种，以减少迟播导致的产量损失。

关键词: 油菜（Brassica napus L.）, 迟播, 叶面积指数, 角果壳面积指数, 光合源演替, 载荷, 产量, 品质

Abstract:

【Objective】 This study aimed to explore the regulation mechanism of late sowing on succession of photosynthetic sources (leaf and silique shell), source loadability, yield and quality in rape. 【Method】 The experiment was conducted in the Dafeng District, Yancheng city of Jiangsu Province, and Nanjing of Jiangsu Province in 2022-2023. The main factors sowing dates were set up at 6 levels, October 17th (SD1), October 23rd (SD2), October 29th (SD3), November 4th (SD4), November 10th (SD5), and November 16th (SD6), and the secondary factors were two conventional varieties: Nannongyou 4 (strong cold resistance) and Zheyou 51 (medium cold resistance). The effects of different sowing dates on dynamic change characteristics of leaf area index (LAI), pod area index (PAI) and source succession, source loadability and rape yield and quality were investigated. 【Result】 (1) The later the sowing dates, the younger the pre-winter seedling age, and the lower the overwintering survival rate. The latest safe date of late-sowing rapeseed in the lower reaches of Yangtze River was the end of October, and the pre-winter seedling age was more than 5 leaves. The sowing date was postponed to November, the leaf age of rape decreased to 1.2-3.6 leaves, and the average overwintering survival rate was less than 30%. The average overwintering rate of Nannongyou 4 in November was 30.9%, which was 14.5% higher than that of Zheyou 51. (2) During the latest safe sowing period, the yield of rapeseed decreased significantly with the delay of sowing dates, and the decrease of plant silique number and 1000-seed weight were the most direct factors causing the decrease of oilseed yield. With the delay of sowing date, compared with SD1, the yield of SD2 and SD3 decreased by 9.6% and 29.0%, the number of plant silique number decreased by 6.1% and 23.9%, and the 1000-seed weight decreased by 4.4% and 6.5%, respectively. The average content of fat and protein in Nannongyou 4 was 42.9% and 25%, which in Zheyou 51 was 47.9% and 22.7%, respectively. (3) With the delay of sowing dates, the maximum LAI and PAI of rape decreased significantly, the slow growth rate before LAI reaching the peak and the fast decline rate after LAI reaching the peak both decreased, and the rapid growth rate of PAI slowed down. The later the sowing dates, the earlier the LAI and PAI succession points (the lower the effective accumulated temperature), and the lower the LAI and PAI succession points (the lower the LAI and PAI succession point). The photosynthetic source and source succession dynamic characteristic values of Nannongyou 4 were generally better than those of Zheyou 51. (4) With the delay of sowing date, the leaf area decreased faster than the seeds yield and pod area, caused the leaf loadability gradually increased, while the silique loadability gradually decreased. Insufficient leaf source and seeds sink were the main reasons for the yield reduction of late sowing. The leaf and shell loadability of Nannongyou 4 was both higher than that of Zheyou 51.【Conclusion】 Within the latest safe sowing period of rapeseed in the lower reaches of Yangtze River, it was advisable to select cold-resistant, high-yield and high-oil varieties with suitable photosynthetic source succession point and high source load, to reduce yield loss caused by late sowing.

Key words: rape (Brassica napus L.), late sowing, leaf area index, pod area index, succession of photosynthetic sources, loadability, yield, quality

李飞, 熊财, 谷佳佳, 曹欣, 王珊珊, 胡伟, 周治国, 陈兵林. 迟播对油菜产量、品质、光合源演替及载荷特征的影响[J]. 中国农业科学, 2024, 57(23): 4673-4685.

LI Fei, XIONG Cai, GU JiaJia, CAO Xin, WANG ShanShan, HU Wei, ZHOU ZhiGuo, CHEN BingLin. Effects of Late Sowing on Yield, Quality, Photosynthetic Source Succession and Loadability Characteristics of Rape[J]. Scientia Agricultura Sinica, 2024, 57(23): 4673-4685.

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参考文献 47

[1]	张琼瑛, 周桂清, 王国槐, 邹应斌. 播种期和播种方式对免耕直播油菜生长与产量的影响. 作物研究, 2004, 18(3): 167-170.
	ZHANG Q Y, ZHOU G Q, WANG G H, ZOU Y B. Study of seeding times and methods on the growth and yield of oilseed rape under the no-tillage condition. Crop Research, 2004, 18(3): 167-170. (in Chinese)
[2]	杨萍, 鲜孟筑, 胡立勇, 徐正华. 低温胁迫对油菜种子萌发及幼苗生长的影响. 华中农业大学学报, 2015, 34(6): 7-13.
	YANG P, XIAN M Z, HU L Y, XU Z H. Effects of low-temperature on seed germination and seedling development of rapeseed. Journal of Huazhong Agricultural University, 2015, 34(6): 7-13. (in Chinese)
[3]	苏伟, 鲁剑巍, 周广生, 李小坤, 韩自航, 雷海霞. 免耕及直播密度对油菜生长、养分吸收和产量的影响. 中国农业科学, 2011, 44(7): 1519-1526. doi: 10.3864/j.issn.0578-1752.2011.07.027.
	SU W, LU J W, ZHOU G S, LI X K, HAN Z H, LEI H X. Effect of no-tillage and direct sowing density on growth, nutrient uptake and yield of rapeseed (Brassica napus L.). Scientia Agricultura Sinica, 2011, 44(7): 1519-1526. doi: 10.3864/j.issn.0578-1752.2011.07.027. (in Chinese)
[4]	TURHAN H, GÜL M K, EGESEL C Ö, KAHRIMAN F. Effect of sowing time on grain yield, oil content, and fatty acids in rapeseed (Brassica napus subsp. oleifera). Turkish Journal of Agriculture and Forestry, 2011, 35(3): 225-234.
[5]	OZER H. Sowing date and nitrogen rate effects on growth, yield and yield components of two summer rapeseed cultivars. European Journal of Agronomy, 2003, 19(3): 453-463.
[6]	SHAFIGHI A, ARDAKANI M R, SHIRANI RAD A H, ALAVIFAZEL M, RAFIEI F. Grain yield and associated physiological traits of rapeseed (Brassica napus L.) cultivars under different planting dates and drought stress at the flowering stage. Italian Journal of Agronomy, 2021, 16(1): 1648.
[7]	蒯婕, 王积军, 左青松, 陈红琳, 高建芹, 汪波, 周广生, 傅廷栋. 长江流域直播油菜密植效应及其机理研究进展. 中国农业科学, 2018, 51(24): 4625-4632. doi: 10.3864/j.issn.0578-1752.2018.24.004.
	KUAI J, WANG J J, ZUO Q S, CHEN H L, GAO J Q, WANG B, ZHOU G S, FU T D. Effects and mechanism of higher plant density on directly-sown rapeseed in the Yangtze River Basin of China. Scientia Agricultura Sinica, 2018, 51(24): 4625-4632. doi: 10.3864/j.issn.0578-1752.2018.24.004. (in Chinese)
[8]	李莉. 播期、密度对油菜产量和品质及生产潜力影响的研究[D]. 武汉: 华中农业大学, 2005.
	LI L. The study on the effect of rapeseed yield, quality and production potential under different sowing date and density[D]. Wuhan: Huazhong Agricultural University, 2005. (in Chinese)
[9]	凌启鸿. 作物群体质量. 上海: 上海科学技术出版社, 2000: 387-389.
	LING Q H. Crop Population Quality. Shanghai: Shanghai Scientific and Technical Publishers, 2000: 387-389. (in Chinese)
[10]	巩若琳, 宋波, 杨志叶, 路丽静, 董军刚. 迟播和密度对不同油菜品种抗倒伏及产量的影响. 作物学报, 2023, 49(10): 2777-2792.
	GONG R L, SONG B, YANG Z Y, LU L J, DONG J G. Effects of sowing date and density on lodging resistance and yield of different rapeseed cultivars. Acta Agronomica Sinica, 2023, 49(10): 2777-2792. (in Chinese) doi: 10.3724/SP.J.1006.2023.24248
[11]	GOFFMAN F D, ALONSO A P, SCHWENDER J, SHACHAR-HILL Y, OHLROGGE J B. Light enables a very high efficiency of carbon storage in developing embryos of rapeseed. Plant Physiology, 2005, 138(4): 2269-2279. pmid: 16024686
[12]	吴永成, 徐亚丽, 彭海浪, 李壮, 牛应泽. 播期及种植密度对直播油菜农艺性状和产量品质的影响. 西南农业学报, 2015, 28(2): 534-538.
	WU Y C, XU Y L, PENG H L, LI Z, NIU Y Z. Effects of sowing date and plant density on agronomic traits, yield and quality of direct-sown oilseed rape (Brassica napus L.) in Sichuan Province. Southwest China Journal of Agricultural Sciences, 2015, 28(2): 534-538. (in Chinese)
[13]	GRAMI B, STEFANSSON B R, BAKER R J. Genetics of protein and oil content in summer rape: Heritability, number of effective factors, and correlations. Canadian Journal of Plant Science, 1977, 57(3): 937-943.
[14]	胡盛, 李阳阳, 唐章林, 李加纳, 曲存民, 刘列钊. 干旱胁迫下甘蓝型油菜籽粒含油量和蛋白质含量变化的全基因组关联分析. 中国农业科学, 2023, 56(1): 17-35. doi: 10.3864/j.issn.0578-1752.2023.01.002.
	HU S, LI Y Y, TANG Z L, LI J N, QU C M, LIU L Z. Genome-wide association analysis of the changes in oil content and protein content under drought stress in Brassica napus L.. Scientia Agricultura Sinica, 2023, 56(1): 17-35. doi: 10.3864/j.issn.0578-1752.2023.01.002. (in Chinese)
[15]	BRUNEL-MUGUET S, BEAUCLAIR P, BATAILLÉ M P, AVICE J C, TROUVERIE J, ETIENNE P, OURRY A. Light restriction delays leaf senescence in winter oilseed rape (Brassica napus L.). Journal of Plant Growth Regulation, 2013, 32(3): 506-518.
[16]	王玲. 油菜光合面积指数消长变化及高产群体指标研究[D]. 武汉: 华中农业大学, 2019.
	WANG L. Study on the dynamic changes of photosythetic area index and the canopy indices of high yield rapeseed[D]. Wuhan: Huazhong Agricultural University, 2019. (in Chinese)
[17]	蔡昆争, 骆世明. 不同生育期遮光对水稻生长发育和产量形成的影响. 应用生态学报, 1999, 10(2): 193-196.
	CAI K Z, LUO S M. Effect of shading on growth, development and yield formation of rice. Chinese Journal of Applied Ecology, 1999, 10(2): 193-196. (in Chinese)
[18]	KHAYAT M, RAHNAMA A, LORZADEH S, LACK S. Physiological indices, phenological characteristics and trait evaluation of canola genotypes response to different planting dates. Proceedings of the National Academy of Sciences of the United States, India Section B: Biological Sciences, 2016, 88(1): 153-163.
[19]	LABRA M H, STRUIK P C, EVERS J B, CALDERINI D F. Plasticity of seed weight compensates reductions in seed number of oilseed rape in response to shading at flowering. European Journal of Agronomy, 2017, 84: 113-124.
[20]	KIRKEGAARD J A, LILLEY J M, MORRISON M J. Drivers of trends in Australian canola productivity and future prospects. Crop and Pasture Science, 2016, 67(4): i-ix.
[21]	王兴才, 杨文钰. 基于间套作弱光胁迫下作物源库协调与产量研究进展. 中国油料作物学报, 2019, 41(2): 292-299. doi: 10.7505/j.issn.1007-9084.2019.02.019
	WANG X C, YANG W Y. Review on relationship of source-sink and crop yield under shading stress in intercropping systems. Chinese Journal of Oil Crop Sciences, 2019, 41(2): 292-299. (in Chinese)
[22]	王瑛, 张胜, 张润生. 植物激素处理对春油菜结实特性的影响. 华北农学报, 2005, 20(S1): 50-53. doi: 10.7668/hbnxb.2005.S1.014
	WANG Y, ZHANG S, ZHANG R S. Effects of plant hormones treament on seeds set of characteristics in rapeseed. Acta Agriculturae Boreali-Sinica, 2005, 20(S1): 50-53. (in Chinese) doi: 10.7668/hbnxb.2005.S1.014
[23]	冷锁虎, 唐瑶, 李秋兰, 左青松, 杨萍. 油菜的源库关系研究 Ⅰ.角果大小对油菜后期源库的调节. 中国油料作物学报, 2005, 27(3): 37-40.
	LENG S H, TANG Y, LI Q L, ZUO Q S, YANG P. Studies on source and sink of rapeseed Ⅰ.Regulation of pod size on source and sink in rapeseed after flowering. Chinese Journal of Oil Crop Scieves, 2005, 27(3): 37-40. (in Chinese)
[24]	中华人民共和国农业农村部公告第12号. 中华人民共和国农业农村部公报, 2018(5): 56.
	Announcement No.12 of the Ministry of Agriculture and Rural Affairs of the People’s Republic of China. Gazette of the Ministry of Agriculture and Rural Affairs of the People’s Republic of China, 2018(5): 56. (in Chinese)
[25]	中华人民共和国农业农村部公告第2566号, 2017. http://www.zzj.moa.gov.cn/gsgg/201712/t20171222_6313696.htm.
	Announcement No. 2566 of the Ministry of Agriculture and Rural Affairs of the People’s Republic of China, 2017. http://www.zzj.moa.gov.cn/gsgg/201712/t20171222_6313696.htm. (in Chinese)
[26]	鲁如坤. 土壤农业化学分析方法. 北京: 中国农业科学技术出版社, 2000.
	LU R K. Methods of Soil Agrochemical Analysis. Beijing: China Agricultural Science and Technology Press, 2000. (in Chinese)
[27]	冷锁虎, 朱耕如. 油菜角果表面积的计算方法. 中国油料, 1991, 13(3): 76-77.
	LENG S H, ZHU G R. Calculation method of surface area of rape horn fruit. Chinese Journal of Oil Crop Sciences, 1991, 13(3): 76-77. (in Chinese)
[28]	LÓPEZ-BASCÓN M A, LUQUE DE CASTRO M D. Soxhlet Extraction. Liquid-Phase Extraction. Amsterdam: Elsevier, 2020: 327-354.
[29]	BOUCHEREAU A, CLOSSAIS-BESNARD N, BENSAOUD A, LEPORT L, RENARD M. Water stress effects on rapeseed quality. European Journal of Agronomy, 1996, 5(1): 19-30.
[30]	王信理. 在作物干物质积累的动态模拟中如何合理运用Logistic方程. 农业气象, 1986, 7(1): 14-19.
	WANG X L. How to use Logistic equation reasonably in dynamic simulation of crop dry matter accumulation. Chinese Journal of Agrometeorology, 1986, 7(1): 14-19. (in Chinese)
[31]	王春丽, 王周礼, 陈婷, 杨建利, 陈文杰, 穆建新, 田建华, 赵小光. 甘蓝型油菜生殖生长期叶片和角果光合与产量关系的研究. 西北植物学报, 2016, 36(7): 1417-1426.
	WANG C L, WANG Z L, CHEN T, YANG J L, CHEN W J, MU J X, TIAN J H, ZHAO X G. Relationship between yield and photosynthesis of leaf and silique of different Brassica napus L. varieties during reproduction period. Acta Botanica Boreali- Occidentalia Sinica, 2016, 36(7): 1417-1426. (in Chinese)
[32]	袁圆, 汪波, 周广生, 刘芳, 黄俊生, 蒯婕. 播期和种植密度对油菜产量和茎秆抗倒性的影响. 中国农业科学, 2021, 54(8): 1613-1626. doi: 10.3864/j.issn.0578-1752.2021.08.004.
	YUAN Y, WANG B, ZHOU G S, LIU F, HUANG J S, KUAI J. Effects of different sowing dates and planting densities on the yield and stem lodging resistance of rapeseed. Scientia Agricultura Sinica, 2021, 54(8): 1613-1626. doi: 10.3864/j.issn.0578-1752.2021.08.004. (in Chinese)
[33]	张树杰, 李玲, 张春雷. 播种期和种植密度对冬油菜籽粒产量和含油率的影响. 应用生态学报, 2012, 23(5): 1326-1332.
	ZHANG S J, LI L, ZHANG C L. Effects of sowing date and planting density on the seed yield and oil content of winter oilseed rape. Chinese Journal of Applied Ecology, 2012, 23(5): 1326-1332. (in Chinese)
[34]	MOMOH E J J, ZHOU W. Growth and yield responses to plant density and stage of transplanting in winter oilseed rape (Brassica napus L.). Journal of Agronomy and Crop Science, 2001, 186(4): 253-259.
[35]	LIERSCH A, BOCIANOWSKI J, NOWOSAD K, MIKOŁAJCZYK K, SPASIBIONEK S, WIELEBSKI F, MATUSZCZAK M, SZAŁA L, CEGIELSKA-TARAS T, SOSNOWSKA K, BARTKOWIAK- BRODA I. Effect of genotype × environment interaction for seed traits in winter oilseed rape (Brassica napus L.). Agriculture, 2020, 10(12): 607.
[36]	OMIDI H, TAHMASEBI Z, ALI NAGHDI BADI H, TORABI H, MIRANSARI M. Fatty acid composition of canola (Brassica napus L.), as affected by agronomical, genotypic and environmental parameters. Comptes Rendus Biologies, 2010, 333(3): 248-254.
[37]	MORSHEDI A. An investigation into the effects of sowing time, N and P fertilizers on seed yield, oil and protein production in canola. Archives of Agronomy and Soil Science, 2011, 57(5): 533-547.
[38]	NAZERI P, SHIRANI RAD A H, VALADABADI S A, MIRAKHORI M, HADIDI MASOULE E. Effect of sowing dates and late season water deficit stress on quantitative and qualitative traits of canola cultivars. Outlook on Agriculture, 2018, 47(4): 291-297.
[39]	张敏, 钟志堂, 金梅, 赵敏, 吴崇友. 江苏省油菜生产现状与问题分析. 中国农机化学报, 2021, 42(8): 209-213, 236. doi: 10.13733/j.jcam.issn.2095-5553.2021.08.28
	ZHANG M, ZHONG Z T, JIN M, ZHAO M, WU C Y. Current status and problems facing rapeseed production in Jiangsu Province. Journal of Chinese Agricultural Mechanization, 2021, 42(8): 209-213, 236. (in Chinese)
[40]	张子龙, 李加纳, 唐章林, 谌利. 播期和密度对甘蓝型黄籽油菜主要品质的影响. 西南农业大学学报(自然科学版), 2005, 27(6): 791-794, 798.
	ZHANG Z L, LI J N, TANG Z L, CHEN L. Effects of sowing date and planting density on the seed colour and the related quality characters of yellow-seeded rapeseed (Brassica napus L.). Journal of Southwest University (Natural Science Edition), 2005, 27(6): 791-794, 798. (in Chinese)
[41]	倪绯. 不同农艺措施对油菜角果碳氮代谢与油脂积累的影响[D]. 武汉: 华中农业大学, 2018.
	NI F. Effect of different agronomic measures on carbon-nitrogen metabolism and oil accumulation in rapeseed pod[D]. Wuhan: Huazhong Agricultural University, 2018. (in Chinese)
[42]	WANG C L, HAI J B, YANG J L, TIAN J H, CHEN W J, CHEN T, LUO H B, WANG H. Influence of leaf and silique photosynthesis on seeds yield and seeds oil quality of oilseed rape (Brassica napus L.). European Journal of Agronomy, 2016, 74: 112-118.
[43]	屠乃美, 官春云. 油菜库器官分化发育期剪叶对源库关系的影响. 湖南农业大学学报(自然科学版), 2001, 27(4): 258-263.
	TU N M, GUAN C Y. Effects on sink-source relationship by leaf-cutting during sink organ differentiation and development of rapeseed. Journal of Hunan Agricultural University, 2001, 27(4): 258-263. (in Chinese)
[44]	戴敬, 郑伟, 喻义珠, 杨举善. 油菜花后光合面积变化及其与产量的关系. 中国油料作物学报, 2001, 23(2): 19-22.
	DAI J, ZHENG W, YU Y Z, YANG J S. The relationship between yield and photosynthetic area after flowering in rapeseed. Chinese Journal of Oil Crop Scieves, 2001, 23(2): 19-22. (in Chinese)
[45]	王春丽, 海江波, 田建华, 杨建利, 赵晓光. 油菜终花后角果和叶片光合对籽粒产量和品质的影响. 西北植物学报, 2014, 34(8): 1620-1626.
	WANG C L, HAI J B, TIAN J H, YANG J L, ZHAO X G. Influence of silique and leaf photosynthesis on yield and quality of seed of oilseed rape (Brassica napus L.) after flowering. Acta Botanica Boreali-Occidentalia Sinica, 2014, 34(8): 1620-1626. (in Chinese)
[46]	刘建国, 帕尼古丽, 董志新, 黄瑛, 张继生, 朱新在. 单位叶面积负荷量对大豆源库调节效应的研究. 石河子大学学报(自然科学版), 2003, 21(4): 259-262.
	LIU J G, PA N, DONG Z X, HUANG Y, ZHANG J S, ZHU X Z. Regulation effect study of unit-leaf-area load on source and sink activity in soybean. Journal of Shihezi University (Natural Science), 2003, 21(4): 259-262. (in Chinese)
[47]	LUO T, ZHANG J, KHAN M N, LIU J H, XU Z H, HU L Y. Temperature variation caused by sowing dates significantly affects floral initiation and floral bud differentiation processes in rapeseed (Brassica napus L.). Plant Science, 2018, 271: 40-51.

地点 Place	品种 Variety	播期 Sowing date
地点 Place	品种 Variety	SD1	SD2	SD3	SD4	SD5	SD6
大丰 Dafeng	V1	7.4a	5.9b	4.7c	3.6d	1.9e	1.2f
大丰 Dafeng	V2	7.6a	5.5b	4.4c	3.6d	1.9e	1.3f
南京 Nanjing	V1	8.8a	7.2c	5.2d	3.3e	1.8f	1.3f
南京 Nanjing	V2	8.7a	7.8b	4.8d	3.3e	1.8f	1.3f

地点 Place	播期 Sowing date	品种 Variety	单株角果数 Plant silique number	每角果粒数 Number of seeds	千粒重 1000-seed weight (g)	产量 Yield (kg·hm^-2)	脂肪含量 Fat content (%)	蛋白质含量 Protein content (%)
大丰 Dafeng	SD1	V1	129.5a	22.3a	4.00a	3814.7a	43.0b	24.8a
	SD1	V2	99.5b	21.9a	3.77b	2693.3c	47.7a	22.5b
	SD2	V1	119.3a	22.9a	3.72bc	3352.8b	42.5b	24.0a
	SD2	V2	100.0b	22.6a	3.55cd	2646.2c	47.5a	21.9b
	SD3	V1	105.0b	21.8a	3.54cd	2694.8c	42.8b	24.7a
	SD3	V2	55.8c	22.7a	3.47d	1445.1d	47.5a	21.6b
南京 Nanjing	SD1	V1	119.5b	22.1a	4.03a	3506.5ab	43.0b	24.9ab
	SD1	V2	130.4a	22.4a	3.88b	3745.4a	48.0a	23.1b
	SD2	V1	117.8ab	21.7a	3.87b	3258.5bc	43.1b	25.6a
	SD2	V2	110.0bc	22.2a	3.84b	3089.3bcd	48.7a	23.1b
	SD3	V1	106.0bc	22.3a	3.81b	2971.2cd	42.9b	25.7a
	SD3	V2	102.5c	21.5a	3.86b	2810.9d	47.8a	23.3b
F值 F-value
地点 Place (P)			**	NS	**	**	**	**
播期 Sowing date (SD)			**	NS	**	**	NS	NS
品种Vairety (V)			**	NS	**	**	**	**
地点×播期 P×SD			*	NS	*	*	NS	NS
地点×品种 P×V			**	NS	*	**	*	NS
播期×品种 SD×V			*	NS	*	NS	NS	NS
地点×播期×品种 P×SD×V			**	NS	NS	*	NS	NS

地点 Place	播期 Sowing date	品种 Variety	回归方程 Regression equation	R²	LAI_M	EAT_LAIM （℃·d）	LAI上升期 LAI ascent stage			LAI下降期 LAI descent stage
地点 Place	播期 Sowing date	品种 Variety	回归方程 Regression equation	R²	LAI_M	EAT_LAIM （℃·d）	MR₁ (/(100 ℃·d))	EAT_M1 (℃·d)	R_avg₁ (/(100 ℃·d))	MR₂ (/(100 ℃·d))	EAT_M2 (℃·d)	R_avg₂ (/(100 ℃·d))
大丰 Dafeng	SD1	V1	LAI=1864.9/（1+e^{1.03×10-8EAT3-5.04×10-6EAT2-0.0082EAT+11.1}）	0.930 **	3.0	701.2	0.93	531.2	0.55	-1.16	872.6	-0.75
	SD1	V2	LAI=1843.3/（1+e^{6.24×10-9EAT3-3.07×10-6EAT2-0.0076EAT+11.4}）	0.969 **	2.7	820.6	0.71	622.6	0.43	-0.87	1020.7	-1.12
	SD2	V1	LAI=1607.1/（1+e^{1.03×10-8EAT3-5.08×10-6EAT2-0.0084EAT+11.3}）	0.801 **	2.4	711.2	0.76	542.5	0.45	-0.93	882.5	-0.60
	SD2	V2	LAI=1645.2/（1+e^{8.84×10-9EAT3-5.16×10-6EAT2-0.0076EAT+11.6}）	0.935 **	2.1	764.7	0.62	586.6	0.37	-0.77	945.6	-0.39
	SD3	V1	LAI=1094.2/（1+e^{1.35×10-8EAT3-6.90×10-6EAT2-0.0092EAT+11.6}）	0.761 **	1.8	677.1	0.63	524.6	0.38	-0.78	832.7	-1.00
	SD3	V2	LAI=1374.7/（1+e^{1.03×10-8EAT3-5.44×10-6EAT2-0.0081EAT+11.7}）	0.891 **	1.4	715.5	0.44	546.5	0.26	-0.55	887.2	-0.35
南京 Nanjing	SD1	V1	LAI=2175.3/（1+e^{4.03×10-9EAT3-1.04×10-6EAT2-0.0076EAT+10.9}）	0.901 **	4.6	885.3	1.11	660.0	0.67	-1.34	1111.3	-0.87
	SD1	V2	LAI=3741.4/（1+e^{2.81×10-9EAT3-1.38×10-6EAT2-0.0051EAT+10.4}）	0.936 **	4.4	957.2	0.91	689.9	0.54	-1.15	1227.0	-0.74
	SD2	V1	LAI=1169.3/（1+e^{4.96×10-9EAT3-8.13×10-7EAT2-0.0094EAT+11.3}）	0.915 **	3.8	852.4	1.02	649.2	0.63	-1.22	1056.4	-0.79
	SD2	V2	LAI=1303.8/（1+e^{3.91×10-9EAT3-1.59×10-6EAT2-0.0063EAT+10.6}）	0.904 **	3.4	882.4	0.77	647.4	0.46	-0.96	1120.7	-0.62
	SD3	V1	LAI=1149.0/（1+e^{5.69×10-9EAT3-1.57×10-6EAT2-0.0092EAT+11.4}）	0.933 **	3.1	832.2	0.85	635.6	0.52	-1.02	1030.8	-0.66
	SD3	V2	LAI=1592.8/（1+e^{4.93×10-9EAT3-2.00×10-6EAT2-0.0074EAT+11.2}）	0.935 **	2.3	854.4	0.58	641.4	0.35	-0.71	1069.7	-0.46

地点 Place	播期 Sowing date	品种 Variety	回归方程 Regression equation	R²	PAI_M	MR₃ (/(100℃·d))	EAT_M3 (℃·d)	EAT₁ (℃·d)	EAT₂ (℃·d)	∆EAT (℃·d)
大丰 Dafeng	SD1	V1	PAI=2.7/（1+e^{-0.023EAT+19.5}）	0.967**	2.7	1.57	841.8	784.9	898.6	113.7
	SD1	V2	PAI=2.4/（1+e^{-0.020EAT+16.9}）	0.971**	2.4	1.07	839.5	774.1	904.8	130.7
	SD2	V1	PAI=2.3/（1+e^{-0.019EAT+14.8}）	0.962**	2.3	0.78	798.6	727.6	869.6	141.9
	SD2	V2	PAI=2.2/（1+e^{-0.019EAT+15.1}）	0.995**	2.2	1.21	798.4	728.8	868.1	139.4
	SD3	V1	PAI=1.8/（1+e^{-0.018EAT+13.8}）	0.963**	1.8	1.01	778.4	704.3	852.5	148.1
	SD3	V2	PAI=1.8/（1+e^{-0.017EAT+12.4}）	0.984**	1.8	0.74	737.9	659.7	816.1	156.3
南京 Nanjing	SD1	V1	PAI=3.2/（1+e^{-0.012EAT+13.1}）	0.964**	3.2	0.99	1050.8	944.7	1156.8	212.1
	SD1	V2	PAI=3.4/（1+e^{-0.014EAT+14.3}）	0.981**	3.4	0.72	1017.8	923.8	1111.8	188.0
	SD2	V1	PAI=2.5/（1+e^{-0.011EAT+11.3}）	0.99**	2.5	0.48	988.2	872.9	1103.4	230.4
	SD2	V2	PAI=3.2/（1+e^{-0.014EAT+13.9}）	0.927**	3.2	1.18	1007.5	912.2	1102.8	190.6
	SD3	V1	PAI=2.4/（1+e^{-0.008EAT+7.8}）	0.966**	2.4	1.11	986.7	819.8	1153.6	333.8
	SD3	V2	PAI=2.8/（1+e^{-0.013EAT+12.0}）	0.988**	2.8	0.71	915.4	814.9	1015.9	200.9