棉花苗期耐低磷种质筛选及耐低磷综合评价

doi:10.3864/j.issn.0578-1752.2023.21.002

中国农业科学 ›› 2023, Vol. 56 ›› Issue (21): 4150-4162.doi: 10.3864/j.issn.0578-1752.2023.21.002

• 作物遗传育种·种质资源·分子遗传学 • 上一篇下一篇

棉花苗期耐低磷种质筛选及耐低磷综合评价

米热扎提江·喀由木¹(), 西尔艾力·吾麦尔江¹, 李晓曈¹, 王香茹¹, 贵会平¹, 张恒恒¹, 张西岭¹, 董强¹^,²(), 宋美珍¹^,²()

¹ 中国农业科学院棉花研究所/棉花生物育种与综合利用全国重点实验室，河南安阳 455000
² 中国农业科学院西部农业研究中心，新疆昌吉 831100

收稿日期:2023-04-18 接受日期:2023-06-02 出版日期:2023-11-01 发布日期:2023-11-06
通信作者:
董强，E-mail：dongqiang@caas.cn。
宋美珍，E-mail：songmzccri@163.com
联系方式: 米热扎提江·喀由木，E-mail：82101205083@caas.cn。
基金资助:
棉花生物学国家重点实验室自主课题(CB2021C10); 新疆生产建设兵团第六师科技项目(2202); 新疆生产建设兵团第一师阿拉尔市科技计划(2022NY09)

Screening of Low Phosphorus Tolerant Germplasm in Cotton at Seedling Stage and Comprehensive Evaluation of Low Phosphorus Tolerance

KAYOUMU MiReZhaTiJiang¹(), WUMAIERJIANG XiErAiLi¹, LI XiaoTong¹, WANG XiangRu¹, GUI HuiPing¹, ZHANG HengHeng¹, ZHANG XiLing¹, DONG Qiang¹^,²(), SONG MeiZhen¹^,²()

¹ Institute of Cotton, Chinese Academy of Agricultural Sciences/National Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Anyang 455000, Henan
² Western Agricultural Research Center of Chinese Academy of Agricultural Sciences, Changji 831100, Xinjiang

Received:2023-04-18 Accepted:2023-06-02 Published:2023-11-01 Online:2023-11-06

摘要/Abstract

摘要：

【目的】建立棉花品种（系）耐低磷能力评价体系，筛选耐低磷型棉花种质和评价不同磷效率类型，为研究棉花耐低磷生理机制和挖掘耐低磷基因奠定基础。【方法】以来自国内外不同棉区的140份棉花品种（系）为材料，采用水培试验方法，在低磷（10 μmol·L^-1 KH₂PO₄）和正常磷（500 μmol·L^-1 KH₂PO₄）处理下，测定各棉花品种生物量、根系相关指标和磷效率相关指标等21个性状表征值，计算各指标耐低磷胁迫指数。利用综合隶属函数法，进行主成分分析、回归分析和聚类分析，对各棉花品种进行耐低磷能力的划分，综合评价各棉花品种耐低磷能力和磷效率类型。【结果】与正常磷处理相比，低磷处理下，供试棉花品种的总磷积累量、总磷含量、地上部干重和总干物重等指标的均值降幅较大，而根平均直径、比根面积、根尖数和磷素利用效率等指标的均值会有所上升；低磷处理下，各指标变异系数范围为6.04%—47.79%，比根尖密度、根尖数、比根长和根平均直径等根系指标变异系数较正常磷处理均提高，变异系数分别为47.49%、42.13%、40.19%和19.16%；对21个指标的耐低磷胁迫指数进行主成分分析，6个主成分的累计方差贡献率达77.21%，利用隶属函数法计算综合耐低磷综合评价值（D）；采用多元回归分析方法，建立D值回归方程，确定6个耐低磷性鉴定指标并进行系统聚类，将不同棉花品种（系）划分为耐低磷型、中间型、低磷敏感型3类。【结论】筛选出棕絮1号、鲁原343、LambrightGL-N、巴西014、南丹里湖大棉、苏远1028和gL2g13等品种为耐低磷型棉花品种，陕2812、FJA、孝2168和东兰那亭大花等品种的耐低磷能力较差，为低磷敏感型；确定总干物重、磷素利用效率、地下部鲜重、总根长、根系表面积和总磷积累量作为棉花耐低磷能力评价的指标。

关键词: 棉花, 耐低磷, 筛选指标, 综合评价, 磷效率

Abstract:

【Objective】To establish an evaluation system for low phosphorus tolerance in cotton varieties (lines), screen low phosphorus tolerant cotton germplasm and evaluate different types of phosphorus efficiency, and lay the foundation for studying the physiological mechanisms of low phosphorus tolerance in cotton and mining low phosphorus tolerance genes.【Method】Using 140 cotton cultivars (lines) from different cotton regions at home and abroad, 21 traits such as biomass, root-related indexes and phosphorus efficiency-related indexes were measured under low (10 μmol·L^-1 KH₂PO₄) and normal (500 μmol·L^-1 KH₂PO₄) phosphorus treatments in a hydroponic experiment. The index of low phosphorus stress tolerance was calculated for each index. Using the integrated affiliation function method, principal component analysis, regression analysis and cluster analysis were conducted to classify the low phosphorus tolerance of each cotton variety and to comprehensively evaluate the low phosphorus tolerance and phosphorus efficiency type of each cotton variety.【Result】Compared with the normal phosphorus treatment, the mean values of total phosphorus accumulation, total phosphorus content, aboveground dry weight and total dry matter weight of the tested cotton varieties decreased more under the low phosphorus treatment, while the mean values of root average diameter, specific root area, root tips number and phosphorus use efficiency increased. Under low phosphorus treatment, the coefficients of variation of each index ranged from 6.04% to 47.79%，the coefficients of variation of root indexes such as specific root tips density, root tips number, specific root length and root average diameter were higher than those of normal phosphorus treatment, and the coefficients of variation were 47.49%, 42.13%, 40.19% and 19.16%, respectively; the principal component analysis of the 21 indexes of low phosphorus stress tolerance showed that the cumulative variance contribution of the six principal components reached 77.21%, and the comprehensive low phosphorus tolerance value (D) was calculated using the affiliation function method. The D-value regression equation was established by multiple regression analysis to determine the six low phosphorus tolerance indices and perform systematic clustering to classify different cotton varieties (lines) into three categories: low phosphorus tolerant, intermediate and low phosphorus sensitive.【Conclusion】Total dry matter weight, phosphorus use efficiency, root fresh weight, total root length, root surface area and total phosphorus accumulation were identified as indicators for the evaluation of low phosphorus tolerance in cotton.

Key words: Gossypium hirsutum L., low phosphorus tolerance, screening index, comprehensive evaluation, phosphorus efficiency

米热扎提江·喀由木, 西尔艾力·吾麦尔江, 李晓曈, 王香茹, 贵会平, 张恒恒, 张西岭, 董强, 宋美珍. 棉花苗期耐低磷种质筛选及耐低磷综合评价[J]. 中国农业科学, 2023, 56(21): 4150-4162.

KAYOUMU MiReZhaTiJiang, WUMAIERJIANG XiErAiLi, LI XiaoTong, WANG XiangRu, GUI HuiPing, ZHANG HengHeng, ZHANG XiLing, DONG Qiang, SONG MeiZhen. Screening of Low Phosphorus Tolerant Germplasm in Cotton at Seedling Stage and Comprehensive Evaluation of Low Phosphorus Tolerance[J]. Scientia Agricultura Sinica, 2023, 56(21): 4150-4162.

图/表 7

表1

2种磷处理下棉花各性状值及耐低磷系数"

性状 Trait	正常磷处理 Normal phosphorus			低磷处理 Low phosphorus			耐低磷系数 LP/CK (%)
性状 Trait	平均值 Mean	标准差 SD	变异系数 CV (%)	平均值 Mean	标准差 SD	变异系数 CV (%)	平均值 Mean	标准差 SD	变异系数 CV
地上部干重ADW (g)	1.70a	0.42	24.81	0.78b	0.18	22.73	48.31	15.54	32.18
地下部干重UDW (g)	0.18b	0.05	26.04	0.22a	0.05	26.69	113.11	41.44	36.64
总干物重TDW (g)	1.88a	0.43	22.90	0.97b	0.21	21.83	54.18	16.48	30.41
主根长TRL (cm)	22.47a	3.73	16.60	22.35a	4.20	18.80	100.71	17.70	17.58
根系表面积RSA (cm²)	212.26b	66.28	31.23	232.29a	71.12	30.62	117.70	50.29	42.73
根平均体积RVE (cm³)	3.01b	1.20	39.86	3.65a	1.17	32.08	136.27	62.30	45.71
总根长TRH (cm)	1226.80a	276.94	22.57	1137.91b	297.77	26.17	97.43	34.04	34.94
根平均直径RAD (mm)	0.55b	0.10	18.80	0.67a	0.13	19.16	123.50	29.60	23.96
比根长SRL (cm·g^-1)	7118.69a	2629.93	36.94	6224.08b	2501.58	40.19	97.60	48.44	49.64
比根面积SRA (cm·g^-2)	1225.44a	577.74	47.15	1254.15a	526.27	41.96	115.23	58.63	50.88
根组织密度RTD (g·cm^-3)	0.07a	0.03	37.89	0.06b	0.02	31.42	98.42	56.73	57.64
根尖数RTN	2182.75b	777.62	35.63	3358.09a	1414.90	42.13	174.14	99.47	57.12
比根尖密度SRTD (个/g)	12586.61b	5817.45	46.22	18046.52a	8623.77	47.79	169.34	105.26	62.16
地上部鲜重SFW (g)	11.87a	2.95	24.83	4.51b	1.12	24.94	40.00	12.94	32.34
地下部鲜重RFW (g)	2.19b	0.75	28.36	2.94a	0.55	25.26	87.86	28.36	32.28
根冠比R/S (%)	11.43b	3.55	31.02	25.78a	5.85	22.70	249.97	107.92	43.17
叶绿素相对含量SPAD	39.06a	1.48	3.80	36.92b	2.23	6.04	94.69	7.05	7.44
总磷含量TPC (mg·g^-1)	0.48a	0.08	17.15	0.07b	0.02	24.02	14.45	4.26	29.50
总磷积累量TPA (g·g^-1)	0.81a	0.24	29.61	0.05b	0.02	29.88	6.97	2.87	41.11
磷素利用效率PUE (g²·mg^-1)	3.68b	1.21	33.02	11.93a	3.15	26.38	354.81	140.05	39.47
磷素吸收效率PUtE (mg·g^-1)	2.15b	0.38	17.47	15.43a	2.59	16.77	737.33	174.98	23.73

表1

图1

表2

图2

表3

图3

图4

参考文献 35

[1]	卢秀茹, 贾肖月, 牛佳慧. 中国棉花产业发展现状及展望. 中国农业科学, 2018, 51(1): 26-36. doi: 10.3864/j.issn.0578-1752.2018.01.003
	LU X R, JIA X Y, NIU J H. The present situation and prospects of cotton industry development in China. Scientia Agricultura Sinica, 2018, 51(1): 26-36. (in Chinese) doi: 10.3864/j.issn.0578-1752.2018.01.003
[2]	庞保刚, 曹楠, 周治国, 赵文青. 不同磷敏感棉花品种临界磷浓度稀释模型与磷营养诊断. 中国农业科学, 2020, 53(22): 4561-4570. doi: 10.3864/j.issn.0578-1752.2020.22.004
	PANG B G, CAO N, ZHOU Z G, ZHAO W Q. Critical phosphorus concentration dilution model and phosphorus nutrition diagnosis in two cotton cultivars with different phosphorus sensitivity. Scientia Agricultura Sinica, 2020, 53(22): 4561-4570. (in Chinese) doi: 10.3864/j.issn.0578-1752.2020.22.004
[3]	白灯莎·买买提艾力, 孙良斌, 冯固. 不同年代棉花品种磷效率比较. 中国生态农业学报(中英文), 2020, 28(7): 1010-1021.
	BAIDENGSHA·M, SUN L B, FENG G. Phosphorus efficiency comparison among cotton varieties cultivated at different times over 63 years. Chinese Journal of Eco-Agriculture, 2020, 28(7): 1010-1021. (in Chinese)
[4]	ZHOU T, DU Y L, AHMED S, LIU T, REN M L, LIU W G, YANG W Y. Genotypic differences in phosphorus efficiency and the performance of physiological characteristics in response to low phosphorus stress of soybean in southwest of China. Frontiers in Plant Science, 2016, 7: 1776. pmid: 27933086
[5]	舒雨. 低磷对小麦叶片生长和光合作用的影响及机理研究[D]. 武汉: 华中农业大学, 2021.
	SHU Y. Studies on the mechanism of the effects of low phosphorus on leaf growth and photosynthesis in wheat[D]. Wuhan: Huazhong Agricultural University, 2021. (in Chinese)
[6]	栗振义, 张绮芯, 仝宗永, 李跃, 徐洪雨, 万修福, 毕舒贻, 曹婧, 何峰, 万里强, 李向林. 不同紫花苜蓿品种对低磷环境的形态与生理响应分析. 中国农业科学, 2017, 50(20): 3898-3907. doi: 10.3864/j.issn.0578-1752.2017.20.006
	LI Z Y, ZHANG Q X, TONG Z Y, LI Y, XU H Y, WAN X F, BI S Y, CAO J, HE F, WAN L Q, LI X L. Analysis of morphological and physiological responses to low Pi stress in different alfalfas. Scientia Agricultura Sinica, 2017, 50(20): 3898-3907. (in Chinese) doi: 10.3864/j.issn.0578-1752.2017.20.006
[7]	刘露露, 汪军成, 姚立蓉, 孟亚雄, 李葆春, 杨轲, 司二静, 王化俊, 马小乐, 尚勋武, 李兴茂. 不同春小麦品种耐低磷性评价及种质筛选. 中国生态农业学报(中英文), 2020, 28(7): 999-1009.
	LIU L L, WANG J C, YAO L R, MENG Y X, LI B C, YANG K, SI E J, WANG H J, MA X L, SHANG X W, LI X M. Evaluation of low phosphorus tolerance and germplasm screening of spring wheat. Chinese Journal of Eco-Agriculture, 2020, 28(7): 999-1009. (in Chinese)
[8]	罗园, 李东梅, 雷淼淼, 冯宗云. 青稞苗期耐低磷能力评价. 麦类作物学报, 2019, 39(12): 1450-1458.
	LUO Y, LI D M, LEI M M, FENG Z Y. Evaluation of low-phosphorus tolerance of hulless barley at seedling stage. Journal of Triticeae Crops, 2019, 39(12): 1450-1458. (in Chinese)
[9]	解斌, 安秀红, 陈艳辉, 程存刚, 康国栋, 周江涛, 赵德英, 李壮, 张艳珍, 杨安. 不同苹果砧木对持续低磷的响应及适应性评价. 中国农业科学, 2022, 55(13): 2598-2612. doi: 10.3864/j.issn.0578-1752.2022.13.010
	XIE B, AN X H, CHEN Y H, CHENG C G, KANG G D, ZHOU J T, ZHAO D Y, LI Z, ZHANG Y Z, YANG A. Response and adaptability evaluation of different apple rootstocks to continuous phosphorus deficiency. Scientia Agricultura Sinica, 2022, 55(13): 2598-2612. (in Chinese) doi: 10.3864/j.issn.0578-1752.2022.13.010
[10]	李银银, 许更文, 李俊峰, 郭佳蓉, 王志琴, 杨建昌. 水稻品种的耐低磷性及其农艺生理性状. 中国水稻科学, 2018, 32(1): 51-66. doi: 10.16819/j.1001-7216.2018.7047
	LI Y Y, XU G W, LI J F, GUO J R, WANG Z Q, YANG J C. Tolerance to low phosphorus and its agronomic and physiological characteristics of rice cultivars. Chinese Journal of Rice Science, 2018, 32(1): 51-66. (in Chinese) doi: 10.16819/j.1001-7216.2018.7047
[11]	林海建, 张志明, 高世斌, 潘光堂. 玉米耐低磷研究现状及磷高效育种策略的探讨. 中国农学通报, 2008, 24(1): 181-185.
	LIN H J, ZHANG Z M, GAO S B, PAN G T. The research actuality of maize (Zea mays L.) tolerance to low phosphorus and the strategy of high phosphorus efficiency breeding. Chinese Agricultural Science Bulletin, 2008, 24(1): 181-185. (in Chinese)
[12]	刘灵, 廖红, 王秀荣, 严小龙. 不同根构型大豆对低磷的适应性变化及其与磷效率的关系. 中国农业科学, 2008, 41(4): 1089-1099.
	LIU L, LIAO H, WANG X R, YAN X L. Adaptive changes of soybean genotypes with different root architectures to low phosphorus availability as related to phosphorus efficiency. Scientia Agricultura Sinica, 2008, 41(4): 1089-1099. (in Chinese)
[13]	郑金凤, 米少艳, 婧姣姣, 白志英, 李存东. 小麦代换系耐低磷生理性状的主成分分析及综合评价. 中国农业科学, 2013, 46(10): 1984-1993. doi: 10.3864/j.issn.0578-1752.2013.10.003
	ZHENG J F, MI S Y, JING J J, BAI Z Y, LI C D. Principal component analysis and comprehensive evaluation on physiological traits of tolerance to low phosphorus stress in wheat substitution. Scientia Agricultura Sinica, 2013, 46(10): 1984-1993. (in Chinese)
[14]	李卫华. 棉花磷素高效利用品种筛选及机理的初步研究[D]. 乌鲁木齐: 新疆农业大学, 2010.
	LI W H. Research on phosphorus efficient genotypes of cotton varieties screening and its mechanism[D]. Urumqi: Xinjiang Agricultural University, 2010. (in Chinese)
[15]	IQBAL A, GUI H P, ZHANG H H, WANG X R, PANG N C, DONG Q A, SONG M Z. Genotypic variation in cotton genotypes for phosphorus-use efficiency. Agronomy, 2019, 9(11): 689. doi: 10.3390/agronomy9110689
[16]	WU A J, FANG Y, LIU S, WANG H, XU B C, ZHANG S Q, DENG X P, PALTA J A, SIDDIQUE K H, CHEN Y L. Root morphology and rhizosheath acid phosphatase activity in legume and graminoid species respond differently to low phosphorus supply. Rhizosphere, 2021, 19: 100391. doi: 10.1016/j.rhisph.2021.100391
[17]	WEN Z H, PANG J Y, TUEUX G, LIU Y F, SHEN J B, RYAN M H, LAMBERS H, SIDDIQUE K H M. Contrasting patterns in biomass allocation, root morphology and mycorrhizal symbiosis for phosphorus acquisition among 20 chickpea genotypes with different amounts of rhizosheath carboxylates. Functional Ecology, 2020, 34(7): 1311-1324. doi: 10.1111/fec.v34.7
[18]	KAYOUMU M, LI X T, IQBAL A, WANG X R, GUI H P, QI Q, RUAN S J, GUO R S, DONG Q, ZHANG X L, SONG M Z. Genetic variation in morphological traits in cotton and their roles in increasing phosphorus-use-efficiency in response to low phosphorus availability. Frontiers in Plant Science, 2022, 13: 1051080. doi: 10.3389/fpls.2022.1051080
[19]	张锡洲, 阳显斌, 李廷轩, 郑子成, 林玲, 杨顺平. 不同磷效率小麦对磷的吸收及根际土壤磷组分特征差异. 中国农业科学, 2012, 45(15): 3083-3092. doi: 10.3864/j.issn.0578-1752.2012.15.009
	ZHANG X Z, YANG X B, LI T X, ZHENG Z C, LIN L, YANG S P. Characteristics of phosphorus uptake and phosphorus fractions in the rhizosphere among different phosphorus efficiency wheat cultivars. Scientia Agricultura Sinica, 2012, 45(15): 3083-3092. (in Chinese)
[20]	OUMA E O. Evaluating heritability and relationships among phosphorus efficiency traits in maize under low P soils of western Kenya. Current Journal of Applied Science and Technology, 2021, 40(11): 83-96.
[21]	罗佳, 候银莹, 程军回, 王宁宁, 陈波浪. 低磷胁迫下不同磷效率基因型棉花的根系形态特征. 中国农业科学, 2016, 49(12): 2280-2289. doi: 10.3864/j.issn.0578-1752.2016.12.004
	LUO J, HOU Y Y, CHENG J H, WANG N N, CHEN B L. Root morphological characteristics of cotton genotypes with different phosphorus efficiency under phosphorus stress. Scientia Agricultura Sinica, 2016, 49(12): 2280-2289. (in Chinese) doi: 10.3864/j.issn.0578-1752.2016.12.004
[22]	龚丝雨, 梁喜欢, 钟思荣, 杨帅强, 张世川, 朱肖文, 王朝, 刘齐元. 苗期耐低磷烟草基因型筛选及其磷效率. 植物营养与肥料学报, 2019, 25(4): 661-670.
	GONG S Y, LIANG X H, ZHONG S R, YANG S Q, ZHANG S C, ZHU X W, WANG Z, LIU Q Y. Screening of tobacco genotypes tolerant to low-phosphorus and their phosphorus efficiency at tobacco seedling stage. Journal of Plant Nutrition and Fertilizers, 2019, 25(4): 661-670. (in Chinese)
[23]	杨春婷, 张永清, 马星星, 陈伟, 董璐, 张楚, 路之娟. 苦荞耐低磷基因型筛选及评价指标的鉴定. 应用生态学报, 2018, 29(9): 2997-3007. doi: 10.13287/j.1001-9332.201809.021
	YANG C T, ZHANG Y Q, MA X X, CHEN W, DONG L, ZHANG C, LU Z J. Screening genotypes and identifying indicators of different Fagopyrum tataricum varieties with low phosphorus tolerance. Chinese Journal of Applied Ecology, 2018, 29(9): 2997-3007. (in Chinese)
[24]	周卫丰, 史春阳, 葛永胜, 丁艳, 胡训霞, 刘璟, 王泽港, 葛才林. 水稻耐低磷根系形态重塑基因挖掘及功能分析. 扬州大学学报 (农业与生命科学版), 2022, 43(3): 1-11.
	ZHOU W F, SHI C Y, GE Y S, DING Y, HU X X, LIU J, WANG Z G, GE C L. Gene mining and functional analysis of root morphological remodeling tolerant to low phosphorus in rice. Journal of Yangzhou University (Agricultural and Life Science Edition), 2022, 43(3): 1-11. (in Chinese)
[25]	刘允熙, 罗佳佳, 雷健, 刘国道, 刘攀道. 柱花草磷高效种质筛选及根系形态对低磷胁迫的响应分析. 草地学报, 2021, 29(5): 876-883. doi: 10.11733/j.issn.1007-0435.2021.05.003
	LIU Y X, LUO J J, LEI J, LIU G D, LIU P D. Screening of phosphorus efficiency germplasm and analysis of root morphology responding to phosphorus deficiency in Stylosanthes guianensis. Acta Agrestia Sinica, 2021, 29(5): 876-883. (in Chinese)
[26]	易科, 杨曙, 孔吴俊, 陈迪文, 谢璐, 唐新莲, 黎晓峰, 赵尊康. 基于根系形态及空间分布的甘蔗磷高效吸收特征分析. 江西农业大学学报, 2022, 44(6): 1362-1372.
	YI K, YANG S, KONG W J, CHEN D W, XIE L, TANG X L, LI X F, ZHAO Z K. Analysis of efficient phosphorus absorption characteristic based on root morphology and spatial distribution in sugarcane. Acta Agriculturae Universitatis Jiangxiensis, 2022, 44(6): 1362-1372. (in Chinese)
[27]	潘新雅, 李军保, 陈阳, 王鹏飞, 卫先伟, 李瑞, 刘佳茜, 郑智龙, 徐炳成, 王智. 6个紫花苜蓿品种根系形态结构对低磷胁迫的响应. 草地学报, 2021, 29(11): 2494-2504. doi: 10.11733/j.issn.1007-0435.2021.11.015
	PAN X Y, LI J B, CHEN Y, WANG P F, WEI X W, LI R, LIU J X, ZHENG Z L, XU B C, WANG Z. Response of root morphology and anatomical structure of six alfalfa cultivars to phosphorus deficiency. Acta Agrestia Sinica, 2021, 29(11): 2494-2504. (in Chinese)
[28]	乔胜锋, 邓亚萍, 瞿寒冰, 张伟杨, 顾骏飞, 张耗, 刘立军, 王志琴, 杨建昌. 不同籼稻品种对低磷响应的差异及其农艺生理性状. 中国水稻科学, 2021, 35(4): 396-406. doi: 10.16819/j.1001-7216.2021.210304
	QIAO S F, DENG Y P, QU H B, ZHANG W Y, GU J F, ZHANG H, LIU L J, WANG Z Q, YANG J C. Differences in response to low phosphorus stress among indica rice varieties and their agronomic and physiological characteristics. Chinese Journal of Rice Science, 2021, 35(4): 396-406. (in Chinese)
[29]	任盼荣, 汪军成, 姚立蓉, 司二静, 杨轲, 孟亚雄, 李葆春, 马小乐, 王化俊. 大麦种质资源磷利用效率的评价及其转录组分析. 大麦与谷类科学, 2018, 35(4): 56-57.
	REN P R, WANG J C, YAO L R, SI E J, YANG K, MENG Y X, LI B C, MA X L, WANG H J. Evaluation of phosphorus use efficiency and transcriptome analysis in barley germplasm (Hordeum vulgare L.). Barley and Cereal Sciences, 2018, 35(4): 56-57. (in Chinese)
[30]	刘鹏, 武爱莲, 王劲松, 南江宽, 董二伟, 焦晓燕, 平俊爱. 不同基因型高粱的磷效率和磷素转运特性研究. 山西农业科学, 2018, 46(3): 344-349.
	LIU P, WU A L, WANG J S, NAN J K, DONG E W, JIAO X Y, PING J A. Study on phosphorus use efficiency and phosphorus remobilization characteristics of four different Sorghum genotypes. Journal of Shanxi Agricultural Sciences, 2018, 46(3): 344-349. (in Chinese)
[31]	李小莉, 胡安永, 王嘉林, 肖珣, 沈仁芳, 赵学强. 酸性土壤上不同水稻品种耐低磷差异研究. 江西农业学报, 2022, 34(5): 102-106.
	LI X L, HU A Y, WANG J L, XIAO X, SHEN R F, ZHAO X Q. Study on difference of low phosphorus tolerance of rice varieties in acid soil. Acta Agriculturae Jiangxi, 2022, 34(5): 102-106. (in Chinese)
[32]	朱瑞利, 毛琳琳, 王龙, 易可可, 孙静文. 苗期耐低磷毛叶苕子品种的筛选及其磷效率类型评价. 中国土壤与肥料, 2022(3): 148-157.
	ZHU R L, MAO L L, WANG L, YI K K, SUN J W. Screening of Vicia Villosa varieties with low phosphorus tolerance at seedling stage and evaluation of their phosphorus efficiency types. Soil and Fertilizer Sciences in China, 2022(3): 148-157. (in Chinese)
[33]	龚丝雨. 烟草苗期耐低磷基因型筛选及生理机制研究[D]. 南昌: 江西农业大学, 2019.
	GONG S Y. Screening of low-phosphorus tolerant genotypes and its physiological mechanism of tobacco at seedling stage[D]. Nanchang: Jiangxi Agricultural University, 2019. (in Chinese)
[34]	张君杰, 朱素青, 骆璐, 张秀荣, 万勇善, 刘风珍, 张昆. 不同花生品种磷吸收速率和根系形态对低磷胁迫的响应. 山东农业科学, 2022, 54(3): 68-73.
	ZHANG J J, ZHU S Q, LUO L, ZHANG X R, WAN Y S, LIU F Z, ZHANG K. Response of phosphorus uptake rate and root morphology to low phosphorus stress in different peanut varieties. Shandong Agricultural Sciences, 2022, 54(3): 68-73. (in Chinese)
[35]	王一凡, 杨江伟, 唐勋, 晋昕, 张宁, 司怀军. 马铃薯响应磷胁迫机制及磷高效利用育种. 中国马铃薯, 2021, 35(1): 68-74.
	WANG Y F, YANG J W, TANG X, JIN X, ZHANG N, SI H J. Mechanism of potato response to phosphorus stress and high- efficiency phosphorus breeding. Chinese Potato Journal, 2021, 35(1): 68-74. (in Chinese)

性状 Trait	主成分 Principal component
性状 Trait	因子1 PC1	因子2 PC2	因子3 PC3	因子4 PC4	因子5 PC5	因子6 PC6
地上部干重ADW	0.40	0.05	0.16	-0.14	0.07	-0.06
地下部干重UDW	0.27	-0.16	-0.35	0.17	-0.06	0.18
总干物重TDW	0.42	0.04	0.10	-0.10	0.05	-0.02
主根长TRL	0.12	0.15	-0.04	0.19	-0.15	-0.14
根系表面积RSA	0.11	0.35	-0.16	0.26	-0.24	-0.03
根平均体积RVE	0.09	0.34	-0.21	0.22	-0.24	0.04
总根长TRH	0.11	0.10	0.18	-0.12	-0.20	0.74
根平均直径RAD	0.00	0.00	0.04	0.01	-0.15	-0.17
比根长SRL	-0.17	0.19	0.36	-0.17	-0.13	0.39
比根面积SRA	-0.16	0.40	0.15	0.08	-0.13	-0.15
根组织密度RTD	0.12	-0.41	-0.08	-0.09	0.19	0.15
根尖数RTN	0.06	0.18	-0.06	0.36	0.58	0.22
地上部鲜重SFW	0.36	0.02	0.06	0.06	-0.03	0.00
比根尖密度SRTD	-0.14	0.25	0.17	0.25	0.55	0.08
地下部鲜重RFW	0.32	0.13	-0.03	0.14	-0.03	0.04
根冠比R/S	-0.06	-0.19	-0.45	0.24	-0.09	0.27
叶绿素相对含量SPAD	0.12	0.00	0.01	-0.11	0.13	-0.17
总磷含量TPC	0.02	-0.24	0.32	0.41	-0.15	-0.03
总磷积累量TPA	0.32	-0.12	0.33	0.16	-0.03	-0.05
磷素利用效率PUE	0.32	0.20	-0.07	-0.33	0.13	-0.05
磷素吸收效率PUtE	-0.02	0.27	-0.35	-0.37	0.12	0.04
方差贡献率 Variance contribution rate (%)	24.04	15.76	13.94	10.94	6.75	5.79
累计贡献率 Cumulative (%)	24.04	39.80	53.74	64.68	71.43	77.21

多元回归方程 Multiple regression equation	决定系数 R²	F值 F value	P值 P value
D=0.13+0.48X₁	0.56	357.79	≤0.001
D=0.12+0.46X₁+0.58X₂	0.65	325.65	≤0.001
D=0.11+0.44X₁+0.55X₂+0.36X₃	0.79	432.59	≤0.001
D=0.11+0.41X₁+0.51X₂+0.32X₃+0.23X₄	0.86	555.48	≤0.001
D=0.09+0.38X₁+0.49X₂+0.29X₃+0.20X₄+0.25X₅	0.91	574.91	≤0.001
D=0.08+0.34X₁+0.46X₂+0.27X₃+0.19X₄+0.23X₅+0.31X₆	0.99	596.43	≤0.001

棉花苗期耐低磷种质筛选及耐低磷综合评价

Screening of Low Phosphorus Tolerant Germplasm in Cotton at Seedling Stage and Comprehensive Evaluation of Low Phosphorus Tolerance

RichHTML

PDF

赞

可视化

摘要/Abstract

引用本文

使用本文

图/表 7

参考文献 35

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	李元晶, 袁瑞祥, 李永泰, 孙天歌, 刘峰, 李艳军, 张新宇. 棉花黄萎病菌β-葡萄糖苷酶基因的鉴定及其在致病中的功能[J]. 中国农业科学, 2026, 59(7): 1380-1399.
[2]	崔节浩, 张萌, 王沁, 于佳妍, 林琨, 李尚泽, 兰恒, 耿艳秋, 张强, 郭丽颖, 邵玺文. 粳稻资源抗倒伏性评价与生理机制[J]. 中国农业科学, 2026, 59(7): 1420-1438.
[3]	郝琨, 陈洪德, 张威, 钟韵, 党美荣, 朱士江, 黄志坤, 金英. 基于柑橘产量、品质及水氮利用的涌泉根灌水氮综合评价[J]. 中国农业科学, 2026, 59(4): 862-873.
[4]	延廷霖, 杜娅丹, 胡笑涛, 王贺, 李晓雁, 王玉明, 牛文全, 谷晓博. 加气滴灌下氮肥有机替代对亏缺灌溉棉花产量和水分利用效率的影响[J]. 中国农业科学, 2026, 59(3): 602-618.
[5]	郭晨荔, 刘扬, 陈燕, 胡伟, 王友华, 周治国, 赵文青. 减氮条件下适当磷肥后移对滴灌棉花产量和磷肥利用效率的影响[J]. 中国农业科学, 2025, 58(9): 1749-1766.
[6]	汪炜檬, 魏云晓, 唐云霓, 刘苗苗, 陈全家, 邓晓娟, 张锐. 棉花发根农杆菌转化体系的建立及生根优化[J]. 中国农业科学, 2025, 58(8): 1479-1493.
[7]	赵宇萱, 苗纪元, 胡伟, 周治国. 苗期低温对棉花花芽分化和棉株产量的影响[J]. 中国农业科学, 2025, 58(7): 1311-1320.
[8]	田立文, 娄善伟, 张鹏忠, 杜明伟, 罗宏海, 李杰, 帕尔哈提·买买提, 马腾飞, 张立祯. 绿色高效生产模式下新疆棉花单产提升面临问题与途径分析[J]. 中国农业科学, 2025, 58(6): 1102-1115.
[9]	陈天晓, 曹榕, 宋倩楠, 胡亮亮, 王素华, 王丽侠, 程须珍, 陈红霖. 小豆种质资源苗期耐盐性综合评价及耐盐种质筛选[J]. 中国农业科学, 2025, 58(21): 4317-4332.
[10]	唐桂梅, 李卫东, 周宇霞, 孔佑涵, 肖晓玲, 彭颖姝, 张力, 符红艳, 刘洋, 黄国林. 基于表型性状分析蕙兰种质资源遗传多样性[J]. 中国农业科学, 2025, 58(2): 339-354.
[11]	包明芳, 秦燕, 陈彩锦, 张尚沛, 张国辉, 沙晓弟. 111份紫花苜蓿种质资源苗期表型抗旱性鉴定评价[J]. 中国农业科学, 2025, 58(19): 3825-3836.
[12]	唐朝元, 刘韬奋, 吴艳琴, 张启鹏, 李自良, 陈云瑞, 雷长英, 张亚黎, 张旺锋, 杜明伟, 杨明凤, 田景山. 陆地棉与海岛棉铃形态特征与纤维及籽仁品质的关系[J]. 中国农业科学, 2025, 58(15): 2980-2992.
[13]	赵甜甜, 袁剑龙, 卓峰琦, 唐振三, 徐杰, 张峰. 马铃薯全粉品质综合评价及品种筛选[J]. 中国农业科学, 2025, 58(13): 2522-2537.
[14]	文晋, 宁艳芳, 秦欣, 刘圆, 张晓玲, 朱永红, 田时敏, 马燕斌. 抗虫与耐草甘膦转基因棉花株系的抗性评价与遗传稳定分析[J]. 中国农业科学, 2025, 58(12): 2291-2302.
[15]	董明, 祁虹, 张谦, 王燕, 王树林, 冯国艺, 梁青龙, 郭宝生. 播种方式对种子萌发环境及棉花出苗、生长的影响[J]. 中国农业科学, 2025, 58(12): 2346-2357.