中国农业科学 ›› 2023, Vol. 56 ›› Issue (3): 466-480.doi: 10.3864/j.issn.0578-1752.2023.03.006
刘瑞1(), 赵羽涵1, 顾欣怡1, 王艳霞1, 靳学慧1, 吴伟怀2, 张亚玲1(
)
收稿日期:
2022-09-28
接受日期:
2022-11-01
出版日期:
2023-02-01
发布日期:
2023-02-14
通信作者:
张亚玲,E-mail:byndzyl@163.com
联系方式:
刘瑞,E-mail:2271031165@qq.com。
基金资助:
LIU RUI1(), ZHAO YuHan1, GU XinYi1, WANG YanXia1, JIN XueHui1, WU WeiHuai2, ZHANG YaLing1(
)
Received:
2022-09-28
Accepted:
2022-11-01
Published:
2023-02-01
Online:
2023-02-14
摘要:
【目的】通过检测黑龙江省和海南省不同稻瘟病菌(Magnaporthe oryzae)菌株群体中AVR-Pita家族的分布情况和变异特征,了解其变异类型的致病表型,为区域内抗病种质的筛选与选育提供参考。【方法】通过参考NCBI中公布的AVR-Pita家族序列分别对启动子区和CDS区设计特异性引物共6对,对2020年采自黑龙江省和海南省不同地区的397个稻瘟病菌单孢菌株提取DNA,进行PCR扩增。通过电泳检测结果,分别选取囊括不同地区的代表菌株对扩增片段进行测序。测序结果与NCBI中相应的碱基与氨基酸序列进行比较分析,并利用水稻抗性单基因系,对不同变异类型的稻瘟病菌菌株进行无毒功能验证。【结果】在PCR电泳检测结果中,黑龙江省稻瘟病菌菌株携带所有待测基因,分布广泛且检出频率较高;而海南省稻瘟病菌菌株中仅携带AVR-Pita1和AVR-Pita2,并以低频率集中存在。无毒基因组成分析结果显示,黑龙江稻区的稻瘟病菌菌株复杂多样,携带的基因型种类较海南菌株丰富。PCR产物测序检测结果显示,AVR-Pita家族以点突变、插入和缺失为主要变异类型划分为19种,且不同稻瘟病菌群体来源的菌株,其变异类型具有特异性。AVR-Pita1检测出10种变异类型,其中AVR-Pita1-(1—5)为黑龙江省稻瘟病菌菌株独有的变异类型,AVR-Pita1-(6—10)为海南省稻瘟病菌菌株独有。对这10种变异类型经功能验证后发现,无毒功能均已丧失。AVR-Pita2检测出8种变异类型,其中AVR-Pita2-(1—4)为黑龙江省稻瘟病菌菌株独有,AVR-Pita2-(5—8)为海南省稻瘟病菌菌株独有。经功能验证后发现,无毒功能均已丧失。AVR-Pita3在黑龙江省仅检测出1种变异类型AVR-Pita3-1。【结论】黑龙江和海南稻瘟病菌群体中AVR-Pita家族均为突变后的等位基因型存在,经致病性鉴定后,检测出的所有突变类型均不能被相对应抗性基因Pi-ta和Pi-ta2所识别,表现为感病。因此,抗性基因Pi-ta和Pi-ta2在黑龙江省和海南省对稻瘟病的抗病育种与利用过程中可聚合其他抗性基因应用来保证品种抗病性。同时,不同地理来源的稻瘟病菌菌株群体中AVR-Pita家族的分布和变异类型具有特异性。
刘瑞, 赵羽涵, 顾欣怡, 王艳霞, 靳学慧, 吴伟怀, 张亚玲. 黑龙江省和海南省稻瘟病菌中AVR-Pita家族的分布及变异分析[J]. 中国农业科学, 2023, 56(3): 466-480.
LIU RUI, ZHAO YuHan, GU XinYi, WANG YanXia, JIN XueHui, WU WeiHuai, ZHANG YaLing. Distribution and Variation Analysis of AVR-Pita Family in Magnaporthe oryzae from Heilongjiang Province and Hainan Province[J]. Scientia Agricultura Sinica, 2023, 56(3): 466-480.
表1
黑龙江省和海南省稻瘟病菌菌株"
省份 Province | 采集地点 Sampling location | 菌株编号 Strain number | 菌株数 Number of strains |
---|---|---|---|
黑龙江省(215a) Heilongjiang Province(215a) | 绥化北林区Beilin District, Suihua | BL20001-BL20006 | 6 |
绥化庆安Qing’an, Suihua | QA20007-QA20009 | 3 | |
绥化青冈Qinggang, Suihua | QG20010-QG20012 | 3 | |
伊春铁力Tieli, Yichun | TL20013-TL20018 | 6 | |
哈尔滨方正Fangzheng, Harbin | FZ20019-FZ20044 | 26 | |
哈尔滨通河Tonghe, Harbin | TH20045-TH20069 | 25 | |
哈尔滨木兰Mulan, Harbin | ML20070-ML20081 | 12 | |
哈尔滨依兰Yilan, Harbin | YL20082-YL20085 | 4 | |
鹤岗绥滨Suibin, Hegang | SB20086-SB20102 | 17 | |
佳木斯富锦Fujin, Jiamusi | FJ20103-FJ20106 | 4 | |
佳木斯东风区Dongfeng District, Jiamusi | DF20199-DF20215 | 17 | |
鸡西虎林Hulin, Jixi | HL20107-HL20110 | 4 | |
鸡西密山Mishan, Jixi | MS20111-MS20169 | 59 | |
牡丹江东宁Dongning, Mudanjiang | DN20170-DN20198 | 29 | |
海南省(182b) Hainan Province(182b) | 东方感城Gancheng, Dongfang | DF20001-DF20004 | 4 |
文昌铺前Puqian, Wenchang | WC20005-WC20038 | 34 | |
定安定城Dingcheng, Dingan | DC20039-DC20065 | 27 | |
定安雷鸣Leiming, Dingan | LM20066-LM20084 | 19 | |
陵水Lingshui | LS20085-LS20094 | 10 | |
澄迈金江Jinjiang, Chengmai | CM20095-CM20182 | 88 |
表2
用于扩增无毒基因的引物"
无毒基因 Avr-gene | 引物序列 Primer sequence (5°-3° | 片段大小 Length of targeted fragments (bp) | 登录号GenBank accession number |
---|---|---|---|
AVR-Pita1 | F1:GCCGAGTCGTTCTGA | 475 | AF207841 |
R1:TGTTAATTGTGCAGAAGTTTTT | |||
F2:TGCCAATAGACTAGCTTCCG | 1957 | ||
R2:ATTCCCTCCATTCCAACACT | |||
AVR-Pita2 | F1:CTTAGAGTAGAATCTTCGTCG | 481 | AB607344 |
R1:GTATGAAAAGGTGCCAATG | |||
F2:TTGGCACCTTTTCATACCCAGTTT | 687 | ||
R2:CAACTTACTTGTGAATCCCATCCC | |||
AVR-Pita3 | F1:AAATATTACCTGCCAGCTGG | 218 | DQ855958 |
R1:TTAAAGCCCCGTGGATATCA | |||
F2:AAATATTACCTGCCAGCTGG | 692 | ||
R2:GCGCTAGTTCATGCATAATC |
图1
AVR-Pita基因家族的PCR扩增电泳图 A:AVR-Pita1启动子区的部分扩增结果Partial amplification results of AVR-Pita1 promoter region;B:AVR-Pita1 CDS区的部分扩增结果Partial amplification of AVR-Pita1 CDS region;C:AVR-Pita2启动子区的部分扩增结果Partial amplification results of AVR-Pita2 promoter region;D:AVR-Pita2 CDS区的部分扩增结果Partial amplification of AVR-Pita2 CDS region;E:AVR-Pita3启动子区的部分扩增结果Partial amplification results of AVR-Pita3 promoter region;F:AVR-Pita3 CDS区的部分扩增结果Partial amplification of AVR-Pita3 CDS region;M:DNA marker DL2000;1:低带Low band;+:存在Existence;-:缺失Missing"
表3
参试菌株携带无毒基因型的检测结果"
基因型 Genotype | 黑龙江Heilongjiang | 海南Hainan | ||
---|---|---|---|---|
菌株数 Number of strains | 频率Frequency (%) | 菌株数 Number of strains | 频率Frequency (%) | |
AVR-Pita(f) | 10 | 4.65 | 92 | 50.55 |
AVR-Pita2 | 3 | 1.40 | 0 | 0 |
AVR-Pita3 | 22 | 10.23 | 0 | 0 |
AVR-Pita1+AVR-Pita2 | 19 | 8.84 | 90 | 49.45 |
AVR-Pita1+AVR-Pita3 | 3 | 1.40 | 0 | 0 |
AVR-Pita2+AVR-Pita3 | 3 | 1.40 | 0 | 0 |
AVR-Pita1+AVR-Pita2+AVR-Pita3 | 155 | 72.09 | 0 | 0 |
AVR-Pita(f):不含待测基因的基因型,其中,f表示不存在 Genotypes that do not contain the gene to be tested, where, f stands for free |
表4
部分菌株AVR-Pita1碱基序列比对结果"
等位基因型 Allele genotype | 碱基突变位点Base mutation site (bp) | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
-413 | -376 | -246 | -116 | -52 | 18—20 | 207 | 245 | 247 | 263 | 312 | 407 | 520 | 575 | 580 | 583 | |
AVR-Pita1 | TGA | TTA | CAA | TAA | AAC | *** | TGT | AAT | GAC | AGA | AAG | GGA | GTT | TAC | TGG | GAT |
AVR-Pita1-2 | -A- | --G | T-- | -C- | --A | GTT | --C | -G- | A-- | -A- | --T | --- | A-- | -G- | --- | --- |
AVR-Pita1-3 | -A- | --G | --- | -C- | --A | GTT | --C | -G- | A-- | -A- | --T | --- | A-- | -G- | --- | --- |
AVR-Pita1-4 | -A- | --G | T-- | -C- | --A | GTT | --C | -G- | A-- | -A- | --T | -A- | A-- | -G- | --- | --- |
AVR-Pita1-5 | -A- | --G | T-- | -C- | --A | GTT | --C | -G- | A-- | -A- | --T | -A- | A-- | --- | --- | --- |
AVR-Pita1-6 | --- | --- | --- | -C- | --A | GTT | --C | -G- | A-- | -A- | --T | -A- | A-- | --- | C-- | --- |
AVR-Pita1-7 | --- | --G | --- | -C- | --A | GTT | --C | -G- | A-- | -A- | --T | -A- | A-- | --- | C-- | --- |
AVR-Pita1-8 | --- | --- | T-- | -C- | --A | GTT | --C | -G- | A-- | -A- | --T | -A- | A-- | --- | C-- | --- |
AVR-Pita1-9 | --- | --- | T-- | --- | --- | GTT | --- | --- | --- | --- | --- | --- | --- | -T- | --- | C-- |
AVR-Pita1-10 | -A- | --G | T-- | -C- | --A | GTT | --- | --- | --- | --- | --- | --- | --- | -T- | --- | C-- |
[1] |
HAYASHI K, YASUDA N, FUJITA Y, KOIZUMI S, YOSHIDA H. Identification of the blast resistance gene Pit in rice cultivars using functional markers. Theoretical and Applied Genetics, 2010, 121(7): 1357-1367.
doi: 10.1007/s00122-010-1393-7 |
[2] | 张亚玲, 高清, 赵羽涵, 刘瑞, 付忠举, 李雪, 孙宇佳, 靳学慧. 黑龙江省水稻种质稻瘟病抗性评价及抗瘟基因结构分析. 中国农业科学, 2022, 55(4): 625-640. |
ZHANG Y L, GAO Q, ZHAO Y H, LIU R, FU Z J, LI X, SUN Y J, JIN X H. Evaluation of rice blast resistance and genetic structure analysis of rice germplasm in Heilongjiang Province. Scientia Agricultura Sinica, 2022, 55(4): 625-640. (in Chinese) | |
[3] |
FLOR H H. Current status of the gene-for-gene concept. Annual Review of Phytopathology, 1971, 9: 275-296.
doi: 10.1146/annurev.py.09.090171.001423 |
[4] |
AZIZI P, RAFII M, ABDULLAH S, NEJAT N, MAZIAH M, HANAFI M, LATIF M, SAHEBI M. Toward understanding of rice innate immunity against Magnaporthe oryzae. Critical Reviews in Biotechnology, 2016, 36(1): 165-174.
doi: 10.3109/07388551.2014.946883 |
[5] | 毛洧, 陈学伟, 王静. 水稻抗稻瘟病机制的研究进展. 中国科学: 生命科学, 2022, 52(10): 1495-1510. |
MAO W, CHEN X W, WANG J. Recent progress on rice resistance to blast disease. Scientia Sinica Vitae, 2022, 52(10): 1495-1510. (in Chinese)
doi: 10.1360/SSV-2021-0012 |
|
[6] | 吴伟怀, 王玲, 程贯忠, 朱有勇, 潘庆华. 稻瘟病菌群体的分子遗传学研究——广东省与云南省稻瘟病菌群体遗传及致病型结构的比较分析. 中国农业科学, 2004, 37(5): 675-680. |
WU W H, WANG L, CHENG G Z, ZHU Y Y, PAN Q H. Studies on molecular genetics of rice blast fungus population——Comparison of genetic and pathotypic structures of two rice blast fungus populations derived from Guangdong and Yunnan provinces of China. Scientia Agricultura Sinica, 2004, 37(5): 675-680. (in Chinese) | |
[7] |
ORBACH M J, FARRALL L, SWEIGARD J A, CHUMLEY F G, VALENT B. A telomeric avirulence gene determines efficacy for the rice blast resistance gene Pi-ta. The Plant Cell, 2000, 12(11): 2019-2032.
doi: 10.1105/tpc.12.11.2019 |
[8] |
KANG S, SWEIGARD J A, VALENT B. The PWL host specificity gene family in the blast fungus Magnaporthe grisea. Molecular Plant-Microbe Interactions, 1995, 8(6): 939-948.
doi: 10.1094/MPMI-8-0939 |
[9] | SWEIGAED J A, CARROLL A M, KANG S, FARRALL L, CHUNLEY F G, VALENT B. Identification, cloning, and characterization of PWL2, a gene for host species specificity in the rice blast fungus. The Plant Cell, 1995, 7(8): 1221-1233. |
[10] |
SCHNEIDER D R S, SARAIVA A M, AZZONI A R, MIRANDA H R, DE TOLEDO M A S, PELLOSO A C, SOUZA A P. Overexpression and purification of PWL2D, a mutant of the effector protein PWL2 from Magnaporthe grisea. Protein Expression and Purification, 2010, 74(1): 24-31.
doi: 10.1016/j.pep.2010.04.020 |
[11] |
COLLEMARE J, PIANFETTI M, HOULLE A E, MORIN D, CAMBORDE L, GAGEY M J, BARBISAN C, FUDAL I, LEBRUN M H, BOHNERT H U. Magnaporthe grisea avirulence gene ACE1 belongs to an infection-specific gene cluster involved in secondary metabolism. New Phytologist, 2008, 179(1): 196-208.
doi: 10.1111/j.1469-8137.2008.02459.x |
[12] |
FARMAN M L, LEONG S A. Chromosome walking to the AVR1-CO39 avirulence gene of Magnaporthe grisea: Discrepancy between the physical and genetic maps. Genetics, 1998, 150(3): 1049-1058.
doi: 10.1093/genetics/150.3.1049 |
[13] |
LI W, WANG B H, WU J, LU G D, HU Y J, ZHANG X, ZHANG Z G, ZHAO Q, FENG Q, ZHANG H Y, WANG Z Y, WANG G L, HAN B, WANG Z H, ZHOU B. The Magnaporthe oryzae avirulence gene AvrPiz-t encodes a predicted secreted protein that triggers the immunity in rice mediated by the blast resistance gene Piz-t. Molecular Plant-Microbe Interactions, 2009, 22(4): 411-420.
doi: 10.1094/MPMI-22-4-0411 |
[14] |
YOSHIDA K, SAITOH H, FUJISAWA S, KANZAKI H, MATSUMURA H, YOSHIDA K, TOSA Y, CHUMA I, TAKANO Y, WIN J, KAMOUN S, TERAUCHI R. Association genetics reveals three novel avirulence genes from the rice blast fungal pathogen Magnaporthe oryzae. The Plant Cell, 2009, 21(5): 1573-1591.
doi: 10.1105/tpc.109.066324 |
[15] |
WU J, KOU Y J, BAO J D, LI Y, TANG M Z, ZHU X L, PONAYA A, XIAO G, LI J B, LI C Y, et al. Comparative genomics identifies the Magnaporthe oryzae avirulence effector AvrPi9that triggers Pi9-mediated blast resistance in rice. New Phytologist, 2015, 206(4): 1463-1475.
doi: 10.1111/nph.13310 |
[16] |
ZHANG S L, WANG L, WU W H, HE L Y, YANG X F, PAN Q H. Function and evolution of Magnaporthe oryzae avirulence gene AvrPib responding to the rice blast resistance gene Pib. Scientific Reports, 2015, 5: 11642.
doi: 10.1038/srep11642 |
[17] |
JIA Y, MCADAMS S A, BRYAN G T, HERSHEY H P, VALENT B. Direct interaction of resistance gene and avirulence gene products confers rice blast resistance. The EMBO Journal, 2000, 19(15): 4004-4014.
doi: 10.1093/emboj/19.15.4004 |
[18] |
KHANG C H, PARK S Y, LEE Y H, VALENT B, KANG S. Genome organization and evolution of the AVR-Pita avirulence gene family in the Magnaporthe grisea species complex. Molecular Plant-Microbe Interactions, 2008, 21(5): 658-670.
doi: 10.1094/MPMI-21-5-0658 |
[19] | 刘松青. 水稻稻瘟病抗性基因Pi-ta2的候选基因筛选与初步分析[D]. 武汉: 中南民族大学, 2019. |
LIU S Q. Screening and preliminary analysis of the candidate genes for rice blast resistance gene Pi-ta2[D]. Wuhan: South-Central University for Nationalities, 2019. (in Chinese) | |
[20] | BRYAN G T, WU K S, FARRALL L, JIA Y, HERSHEY H P, MCADAMS S A, FAULK K N, DONALDSON G K, TARCHINI R, VALENT B. A single amino acid difference distinguishes resistant and susceptible alleles of the rice blast resistance gene Pi-ta. The Plant Cell, 2000, 12(11): 2033-2045. |
[21] | 甘玉姿, 肖贵, 邓启云, 吴俊, 柏斌, 卢向阳, 周波. 菲律宾稻瘟病菌生理小种中AVR-Pita及其同源基因的序列与功能分析. 中国生物防治学报, 2018, 34(3): 488-498. |
GAN Y Z, XIAO G, DENG Q Y, WU J, BAI B, LU X Y, ZHOU B. The sequence and function analysis of AVR-Pita gene family of rice blast fungus, Magnaporthe oryzae in Philippines. Chinese Journal of Biological Control, 2018, 34(3): 488-498. (in Chinese) | |
[22] | 余欢, 姜华, 王艳丽, 孙国昌. 无毒基因在不同寄主梨孢菌中的变异研究. 浙江农业学报, 2015, 27(8): 1414-1421. |
YU H, JIANG H, WANG Y L, SUN G C. Variability of avirulence genes in Pyricularia isolates from different hosts. Acta Agriculturae Zhejiangensis, 2015, 27(8): 1414-1421. (in Chinese) | |
[23] | 刘瑞, 赵羽涵, 付忠举, 顾欣怡, 王艳霞, 靳学慧, 杨莹, 吴伟怀, 张亚玲. 黑龙江省和海南省PWL基因家族在稻瘟病菌中的分布及变异. 中国农业科学, 2023, 56(2): 264-274. |
LIU R, ZHAO Y H, FU Z J, GU X Y, WANG Y X, JIN X H, YANG Y, WU W H, ZHANG Y L. Distribution and variation of PWL gene family in rice Magnaporthe oryzae from Heilongjiang Province and Hainan Province. Scientia Agricultura Sinica, 2023, 56(2): 264-274. (in Chinese) | |
[24] | 李焕宇, 付婷婷, 张云, 吕天佑, 李远, 徐秉良. 5种方法提取真菌基因组DNA作为PCR模板效果的比较. 中国农学通报, 2017, 33(16): 28-35. |
LI H Y, FU T T, ZHANG Y, LÜ T Y, LI Y, XU B L. Effect comparison of five methods to extract fungal genomic DNA as PCR templates. Chinese Agricultural Science Bulletin, 2017, 33(16): 28-35. (in Chinese) | |
[25] |
孟峰, 张亚玲, 靳学慧. 黑龙江省稻瘟病菌无毒基因AVR-Pita及其同源基因的检测与分析. 中国水稻科学, 2020, 34(2): 143-149.
doi: 10.16819/j.1001-7216.2020.9085 |
MENG F, ZHANG Y L, JIN X H. Detection and analysis of Magnaporthe oryzae avirulent gene AVR-Pita and its homologous genes in Heilongjiang Province. Chinese Journal of Rice Science, 2020, 34(2): 143-149. (in Chinese) | |
[26] |
SHI N N, RUAN H C, LIU X Z, YANG X J, DAI Y L, GAN L, CHEN F R, DU Y X. Virulence structure of Magnaporthe oryzae populations from Fujian Province, China. Canadian Journal of Plant Pathology, 2018, 40(4): 542-550.
doi: 10.1080/07060661.2018.1504821 |
[27] | 靳学慧. 农业植物病理学. 赤峰: 内蒙古科学技术出版社, 1999. |
JIN X H. Agricultural Plant Pathology. Chifeng: Inner Mongolia Science and Technology Press, 1999. (in Chinese) | |
[28] | 李思博, 魏松红, 王海宁, 罗文芳, 张优, 刘志恒. 2015-2016年辽宁省稻瘟病菌种群动态分析. 沈阳农业大学学报, 2017, 48(3): 284-289. |
LI S B, WEI S H, WANG H N, LUO W F, ZHANG Y, LIU Z H. Dynamics of rice blast fungus population in Liaoning Province in 2015-2016. Journal of Shenyang Agricultural University, 2017, 48(3): 284-289. (in Chinese) | |
[29] |
VALENT B, KHANG C H. Recent advances in rice blast effector research. Current Opinion in Plant Biology, 2010, 13(4): 434-441.
doi: 10.1016/j.pbi.2010.04.012 pmid: 20627803 |
[30] | 廖静静, 谢华, 王殿东, 何霞红. 云南省元阳县籼/粳型稻瘟病菌无毒基因Avr-Pia、Avr-Pita1和Avr-Pii多样性分析. 植物保护学报, 2019, 46(5): 1057-1064. |
LIAO J J, XIE H, WANG D D, HE X H. Polymorphism analysis of Avr-Pia, Avr-Pita1 and Avr-Pii in indica/japonica-borne Magnaporthe oryzae isolates in Yuanyang County, Yunnan. Journal of Plant Protection, 2019, 46(5): 1057-1064. (in Chinese) | |
[31] | 房文文. 稻瘟菌群体无毒基因型的时空动态研究[D]. 北京: 中国农业大学, 2018. |
FANG W W. Temporal and spatial dynamics of avirulence genotypes in the populations of Pyricularia oryzae[D]. Beijing: China Agricultural University, 2018. (in Chinese) | |
[32] | 王世维, 郑文静, 赵家铭, 魏松红, 王妍, 赵宝海, 刘志恒. 辽宁省稻瘟病菌无毒基因型鉴定及分析. 中国农业科学, 2014, 47(3): 462-472. |
WANG S W, ZHENG W J, ZHAO J M, WEI S H, WANG Y, ZHAO B H, LIU Z H. Identification and analysis of Magnaporthe oryzae avirulence genes in Liaoning Province. Scientia Agricultura Sinica, 2014, 47(3): 462-472. (in Chinese) | |
[33] | 周江鸿, 王久林, 蒋琬如, 雷财林, 凌忠专. 我国稻瘟病菌毒力基因的组成及其地理分布. 作物学报, 2003, 29(5): 646-651. |
ZHOU J H, WANG J L, JIANG W R, LEI C L, LING Z Z. Virulence genes diversity and geographic distribution of Pyricularia grisea in China. Acta Agronomica Sinica, 2003, 29(5): 646-651. (in Chinese) | |
[34] | 高清, 张亚玲, 周弋力, 于连鹏, 聂强, 靳学慧. 黑龙江省粳稻品种稻瘟病主效抗性基因鉴定与抗性评价. 作物杂志, 2021(4): 59-66. |
GAO Q, ZHANG Y L, ZHOU Y L, YU L P, NIE Q, JIN X H. Identification of major resistance genes and resistance evaluation to rice blast in japonica rice varieties in Heilongjiang Province. Crops, 2021(4): 59-66. (in Chinese) | |
[35] | 相亚超, 王丽丽, 徐凡, 马殿荣. 抗稻瘟病基因在黑龙江水稻资源中的分布. 分子植物育种, 2018, 16(23): 7705-7717. |
XIANG Y C, WANG L L, XU F, MA D R. Study on the distribution of rice blast resistant genes in rice resources of Heilongjiang Province. Molecular Plant Breeding, 2018, 16(23): 7705-7717. (in Chinese) | |
[36] | 唐雪婷. 黑龙江省四个稻瘟病流行生态区稻瘟病菌无毒基因动态分析[D]. 大庆: 黑龙江八一农垦大学, 2021. |
TANG X T. Dynamic analysis of avirulence genes of Maghaporthe oryzae in four epidemic ecological regions of Heilongjiang Province[D]. Daqing: Heilongjang Bayi Agricultural University, 2021. (in Chinese) | |
[37] | 董丽英, 王群, 刘树芳, 郑凤萍, 李迅东, 杨勤忠. 云南省稻瘟病菌群体对稻瘟病抗性单基因系的致病性分析. 西南农业学报, 2012, 25(2): 467-473. |
DONG L Y, WANG Q, LIU S F, ZHENG F P, LI X D, YANG Q Z. Pathogenicity analysis of Magnaporthe oryzae populations of Yunnan on monogenic lines for resistance to rice blast. Southwest China Journal of Agricultural Sciences, 2012, 25(2): 467-473. (in Chinese) | |
[38] | 曹雪琦. 福建省稻瘟病菌田间种群无毒基因的变异检测[D]. 福建: 福建农林大学, 2020. |
CAO X Q. Detection of avirulence gene mutations in the field population of rice blast flungus in Fujian Province[D]. Fujian: Fujian Agriculture and Forestry University, 2020. (in Chinese) | |
[39] | 周瑚, 任佐华, 王恒沪, 张译允, 邹秋霞, 刘二明. 湖南桃江病圃稻瘟病菌的无毒基因及水稻抗瘟单基因联合抗性分析. 微生物学通报, 2017, 44(10): 2353-2360. |
ZHOU H, REN Z H, WANG H H, ZHANG Y Y, ZOU Q X, LIU E M. Analysis of avirulence genes of Magnaporthe oryzae and resistance association of monogene against blast from rice blast nursery in Hunan Taojiang. Microbiology China, 2017, 44(10): 2353-2360. (in Chinese) | |
[40] | 姜华, 余欢, 王艳丽, 孙国昌. 稻瘟病菌无毒基因序列变异研究进展. 浙江农业学报, 2015, 27(3): 512-520. |
JIANG H, YU H, WANG Y L, SUN G C. Progress on sequence variation of avirulence genes in the rice blast fungus Magnaporthe grisea. Acta Agriculturae Zhejiangensis, 2015, 27(3): 512-520. (in Chinese) | |
[41] |
SIRISATHAWORN T, SRIRAT T, LONGYA A, JANTASURIYARAT C. Evaluation of mating type distribution and genetic diversity of three Magnaporthe oryzae avirulence genes, PWL-2, AVR-Pii and Avr-Piz-t, in Thailand rice blast isolates. Agriculture and Natural Resources, 2017, 51(1): 7-14.
doi: 10.1016/j.anres.2016.08.005 |
[42] |
KASETSOMBOON T, KATE-NGAM S, SRIWONGCHAI T, ZHOU B, JANTASURIYARAT C. Sequence variation of avirulence gene AVR-Pita1 in rice blast fungus, Magnaporthe oryzae. Mycological Progress, 2013, 12(4): 617-628.
doi: 10.1007/s11557-012-0867-1 |
[43] |
ZHOU E, JIA Y, SINGH P, CORRELL J C, LEE F N. Instability of the Magnaporthe oryzae avirulence gene AVR-Pita alters virulence. Fungal Genetics and Biology, 2007, 44(10): 1024-1034.
doi: 10.1016/j.fgb.2007.02.003 |
[44] |
DAI Y, JIA Y, CORRELL J, WANG X, WANG Y. Diversification and evolution of the avirulence gene AVR-Pita1 in field isolates of Magnaporthe oryzae. Fungal Genetics and Biology, 2010, 47(12): 973-980.
doi: 10.1016/j.fgb.2010.08.003 |
[45] |
CHUMA I, ISOBE C, HOTTA Y, IBARAGI K, FUTAMATA N, KUSABA M, YOSHIDA K, TERAUCHI R, FUJITA Y, NAKAYASHIKI H, VALENT B, TOSA Y. Multiple translocation of the AVR-Pita effector gene among chromosomes of the rice blast fungus Magnaporthe oryzae and related species. PLoS Pathogens, 2011, 7(7): e1002147.
doi: 10.1371/journal.ppat.1002147 |
[46] | 肖丹凤. 三个不同稻作区稻瘟病菌致病性与品种互作研究[D]. 北京: 中国农业科学院, 2013. |
XIAO D F. Studies on the pathogenicity of Maghaporthe oryzae in three different rice growing regions and their interactions to rice varieties[D]. Beijing: Chinese Academy of Agricultural Sciences, 2013. (in Chinese) | |
[47] |
HUANG J, SI W, DENG Q, LI P, YANG S. Rapid evolution of avirulence genes in rice blast fungus Magnaporthe oryzae. BMC Genetics, 2014, 15: 45.
doi: 10.1186/1471-2156-15-45 |
[48] |
ZHANG Y L, ZHU Q L, YAO Y X, ZHAO Z H, CORRELL J C, WANG L, PAN Q H. The race structure of the rice blast pathogen across southern and northeastern China. Rice, 2017, 10(1): 46.
doi: 10.1186/s12284-017-0185-y pmid: 28983868 |
[1] | 刘瑞, 赵羽涵, 付忠举, 顾欣怡, 王艳霞, 靳学慧, 杨莹, 吴伟怀, 张亚玲. 黑龙江省和海南省PWL基因家族在稻瘟病菌中的分布及变异[J]. 中国农业科学, 2023, 56(2): 264-274. |
[2] | 汪文娟,苏菁,陈深,杨健源,陈凯玲,冯爱卿,汪聪颖,封金奇,陈炳,朱小源. 广东省侵染美香占2号的稻瘟病菌致病性及无毒基因变异分析[J]. 中国农业科学, 2022, 55(7): 1346-1358. |
[3] | 郭燕, 张树航, 李颖, 张馨方, 王广鹏. 中国板栗36个叶片表型性状的多样性[J]. 中国农业科学, 2022, 55(5): 991-1009. |
[4] | 黄勋和,翁茁先,李威娜,王庆,何丹林,罗威,张细权,杜炳旺. 中国地方品种黄鸡线粒体DNA D-loop遗传多样性研究[J]. 中国农业科学, 2022, 55(22): 4526-4538. |
[5] | 万映伶,朱梦婷,刘爱青,金亦佳,刘燕. 中国观赏芍药表型多样性解析与资源评价[J]. 中国农业科学, 2022, 55(18): 3629-3639. |
[6] | 郭迎新,陈永亮,苗琪,范志勇,孙军伟,崔振岭,李军营. 洱海流域植烟土壤养分时空变异特征及肥力评价[J]. 中国农业科学, 2022, 55(10): 1987-1999. |
[7] | 吴云雨,肖宁,余玲,蔡跃,潘存红,李育红,张小祥,黄年生,季红娟,戴正元,李爱宏. 长江下游粳稻稻瘟病广谱抗性基因组合模式分析[J]. 中国农业科学, 2021, 54(9): 1881-1893. |
[8] | 陈璨,韩南南,刘洋,史晓维,司红起,马传喜. 小麦Glu-3位点基因拷贝数的变异分析[J]. 中国农业科学, 2021, 54(6): 1092-1103. |
[9] | 李紫腾,曹钰晗,李楠,孟祥龙,胡同乐,王树桐,王亚南,曹克强. 苹果锈果类病毒在7个品种苹果上的分子变异及系统发育关系[J]. 中国农业科学, 2021, 54(20): 4326-4336. |
[10] | 刘有春,刘威生,王兴东,孙斌,刘修丽,杨艳敏,魏鑫,杨玉春,张舵,刘成,李天忠. 基于简化基因组测序的越橘杂交后代鉴定[J]. 中国农业科学, 2021, 54(2): 370-378. |
[11] | 张林林,智慧,汤沙,张仁梁,张伟,贾冠清,刁现民. 谷子抽穗时间基因SiTOC1的表达与单倍型变异分析[J]. 中国农业科学, 2021, 54(11): 2273-2286. |
[12] | 盖钧镒,贺建波. 限制性两阶段多位点全基因组关联分析法(RTM-GWAS)的特点、常见提问与应用前景[J]. 中国农业科学, 2020, 53(9): 1699-1703. |
[13] | 贺建波,刘方东,王吴彬,邢光南,管荣展,盖钧镒. 限制性两阶段多位点全基因组关联分析法在遗传育种中的应用[J]. 中国农业科学, 2020, 53(9): 1704-1716. |
[14] | 普雪可,吴春花,勉有明,苗芳芳,侯贤清,李荣. 不同覆盖方式对旱作马铃薯生长及土壤水热特征的影响[J]. 中国农业科学, 2020, 53(4): 734-747. |
[15] | 赵晴月,许世杰,张务帅,张哲,姚智,陈新平,邹春琴. 中国玉米主产区土壤养分的空间变异及影响因素分析[J]. 中国农业科学, 2020, 53(15): 3120-3133. |
|