转录组及代谢组联合解析茄子果色上位遗传效应

doi:10.3864/j.issn.0578-1752.2022.20.011

中国农业科学 ›› 2022, Vol. 55 ›› Issue (20): 3997-4010.doi: 10.3864/j.issn.0578-1752.2022.20.011

转录组及代谢组联合解析茄子果色上位遗传效应

孙保娟¹(),汪瑞²,孙光闻²,王益奎³,李涛¹,宫超¹,衡周¹,游倩¹,李植良¹()

¹广东省农业科学院蔬菜研究所/广东省蔬菜新技术研究重点实验室,广州 510640
²华南农业大学园艺学院,广州 510642
³广西壮族自治区农业科学院蔬菜研究所,南宁 530027

收稿日期:2022-01-20 接受日期:2022-06-06 出版日期:2022-10-16 发布日期:2022-10-24
通讯作者: 李植良
作者简介:孙保娟,Tel：020-38469456;E-mail： sunbaojuan@hotmail.com。
基金资助:
国家自然科学基金(31501755);广东省现代农业产业技术体系(2021KJ110);广东省现代农业产业技术体系(2021KJ106);广东省自然科学基金(2021A1515012490);广东省农业科学院农业优势产业学科团队建设项目(202114TD);广东省农业科学院农业优势产业学科团队建设项目(R2020PY-JX003)

Transcriptome and Metabolome Integrated Analysis of Epistatic Genetics Effects on Eggplant Peel Color

SUN BaoJuan¹(),WANG Rui²,SUN GuangWen²,WANG YiKui³,LI Tao¹,GONG Chao¹,HENG Zhou¹,YOU Qian¹,LI ZhiLiang¹()

¹Vegetable Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory for New Technology Research of Vegetables, Guangzhou 510640
²College of Horticulture, South China Agricultural University, Guangzhou 510642
³Institute of Vegetable, Guangxi Academy of Agricultural Sciences, Nanning 530007

Received:2022-01-20 Accepted:2022-06-06 Online:2022-10-16 Published:2022-10-24
Contact: ZhiLiang LI

摘要/Abstract

摘要：

【目的】茄子果色与商品果外观品质和价值密切相关,花青素是决定茄子果色的重要天然色素之一。通过基因表达和代谢物差异比较,解析上位基因互作调控茄子果皮花青素合成的作用机制,为不同果色茄子选育提供理论基础。【方法】以花青素合成结构基因ANS突变的白果色母本19141、花青素合成关键调控基因MYB1突变的白果色父本19147及其紫红果色F₁代E3316茄子为试验材料,对商品果期果皮进行转录组测序和广靶代谢组分析。【结果】转录组测序分析表明,19141_vs_19147的差异表达基因（DEGs）最多,其次是E3316_vs_19141,两个比较组DEGs均在类黄酮途径富集程度最高;E3316_vs_19147筛选到的DEGs最少,未在类黄酮途径富集。广靶代谢组共检测分析到218个代谢物。E3316_vs_19141共检测到差异代谢物（DAMs）113个,E3316_vs_19147共检测到差异代谢物98个。转录组和代谢组联合分析发现花青素生物合成关键结构基因CHS、CHI、F3H、DFR和ANS,关键调节基因MYB1、AN1和AN11,修饰基因3GT、5GT、AT和OMT,还有转运基因AN9（GST）相对表达量均为19141>E3316>19147;果皮呈色相关的花青素类代谢物矢车菊素（CAS：528-58-5）、矢车菊素3,5-二葡萄糖苷（CAS：20905-74-2）含量为E3316>19147>19141。上位基因调控下,茄子果皮中同时表达F3′H与F3′5′H,但是F3′H表达水平远高于F3′5′H。E3316花青素含量高于亲本,绿原酸含量低于其亲本。【结论】上位性基因互作控制果色的遗传背景下,亲本突变基因类型决定了花青素代谢通路基因表达趋势和果皮呈色抑制方式。两个白果色亲本杂交F₁代果皮呈紫红色是由于花青素生物合成途径两个互作突变基因位点功能互补;F3′H高水平表达是合成矢车菊型花青素的主要原因;果皮花青素和绿原酸生物合成间存在竞争关系。

关键词: 茄子, 果色, 上位基因, 花青素, 转录组, 代谢组

Abstract:

【Objective】Peel color is closely related to the appearance and value of eggplant. Anthocyanin is one of the natural pigments that determines an eggplant’s peel color. Through the comparison of gene expression and metabolites as well as the analysis on the mechanism of epistatic gene interaction and regulation of anthocyanin synthesis in eggplant peel, this paper provided a theoretical basis for eggplant breeding with different peel colors.【Method】The white-peel female parent 19141 with mutation at the structural gene ANS of anthocyanin biosynthesis pathway, white-peel male parent 19147 with mutation at regulatory gene MYB1 of anthocyanin biosynthesis pathway, and their F₁hybrid E3316 with reddish-purple-peel were used as test materials, while transcriptome sequencing and wide-targeted metabolome analysis were performed on peels of commercial eggplant.【Result】The transcriptome sequencing analysis showed that: 19141_vs_19147 had the most differentially expressed genes (DEGs), followed by E3316_vs_19141. The two comparison groups both had the significant enrichment of DEGs in the flavonoid pathway. The DEGs of E3316_vs_19147 were the least and were not enriched in the flavonoid pathway. A total of 218 metabolites were detected by wide-targeted metabolome analysis. A total of 113 differential accumulated metabolites (DAMs) were detected in E3316_vs_19141, and total 98 DAMs were detected in E3316_vs_19147. The combined analysis of transcriptome and metabolome found that the relative expression levels of key structural genes CHS, CHI, F3H, DFR and ANS, key regulatory genes MYB1, AN1 and AN11, modifier genes 3GT, 5GT, AT and OMT, and transporter gene AN9 (GST) involving anthocyanin biosynthesis pathway had the relation of 19141>E3316>19147. The contents of anthocyanin metabolite cyanidin (CAS: 528-58-5) and cyanin (CAS: 20905-74-2) related to peel coloration had the relation of E3316>19147>19141. The combined analysis of transcriptome and metabolome both showed that the difference of E3316_vs_19141 was bigger than E3316_vs_19147. Under the regulation of epistatic genes, F3'H and F3'5'H genes were expressed simultaneously in eggplant peel, but the expression level of F3'H was much higher than that of F3'5'H. The anthocyanin content of E3316 was higher than that of its parents, while the chlorogenic acid content was lower than that of its parents. 【Conclusion】Under the interaction of epistatic genes with controlling the peel color, the locus (type) and mutation mode of mutant gene in parent determined the trend of gene expression in the whole anthocyanin metabolism pathway and the inhibition mode of peel pigmentation. The reddish-purple-peel F₁ hybrid of two white-peel parents was the result of the function complementation of two mutated gene involved in anthocyanin biosynthesis pathway. The highly expressed F3'H was the main cause for synthesis of cyanidin, and the relationship between anthocyanin and chlorogenic acid biosynthesis was competitive in peels.

Key words: eggplant, peel color, epistatic genes, anthocyanins, transcriptome, metabolome

孙保娟,汪瑞,孙光闻,王益奎,李涛,宫超,衡周,游倩,李植良. 转录组及代谢组联合解析茄子果色上位遗传效应[J]. 中国农业科学, 2022, 55(20): 3997-4010.

SUN BaoJuan,WANG Rui,SUN GuangWen,WANG YiKui,LI Tao,GONG Chao,HENG Zhou,YOU Qian,LI ZhiLiang. Transcriptome and Metabolome Integrated Analysis of Epistatic Genetics Effects on Eggplant Peel Color[J]. Scientia Agricultura Sinica, 2022, 55(20): 3997-4010.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2022.20.011

https://www.chinaagrisci.com/CN/Y2022/V55/I20/3997

图/表 8

图1

图2

图3

表1

图4

图5

图6

图7

参考文献 31

[1]	MIKO B I, WRITE P D, RIGHT S, EDUCATION N. Epistasis: Gene interaction and phenotype effects. Nature Education, 2011, 6(2008): 1-6.
[2]	TIGCHELAAR E C, JANICK J, ERICHSON H T. The genetics of anthocyanin coloration in eggplant (Solanum melongena L.). Genetics, 1968, 60: 475-491. doi: 10.1093/genetics/60.3.475. doi: 10.1093/genetics/60.3.475
[3]	NUNOME T, ISHIGURO K, YOSHIDA T, HIRAI M. Mapping of fruit shape and color development traits in eggplant (Solanum melongena L.) based on RAPD and AFLP markers. Breeding Science, 2011, 51: 19-26. doi: 10.1270/jsbbs.51.19. doi: 10.1270/jsbbs.51.19
[4]	DOGANLAR S, FRARY A, DAUNAY M C, LESTER R N, TANKSLEY S D. Conservation of gene function in the Solanaceae as revealed by comparative mapping of domestication traits in eggplant. Genetics, 2002, 161(4): 1713-1726. doi: 10.1093/genetics/161.4.1713. doi: 10.1093/genetics/161.4.1713 pmid: 12196413
[5]	庞文龙, 刘富中, 陈钰辉, 连勇. 茄子果色性状的遗传研究. 园艺学报, 2008, 35(7): 979-986.
	PANG W L, LIU F Z, CHEN Y H, LIAN Y. Genetic study on fruit color traits of eggplant. Acta Horticulturae Sinica, 2008, 35(7): 979-986. (in Chinese)
[6]	LIAO Y, SUN B J, SUN G W, LIU H C, LI Z L, LI Z X, WANG G P, CHEN R Y. AFLP and SCAR markers associated with peel color in eggplant (Solanum melongena L.). Scientia Agricultura Sinica, 2009, 8(12): 1466-1474.
[7]	HOLTON T A, CORNISH E C. Genetics and biochemistry of anthocyanin biosynthesis. The Plant Cell, 1995, 7(7): 1071-1083. doi: 10.1105/tpc.7.7.1071. doi: 10.1105/tpc. 7.7.1071 pmid: 12242398
[8]	宫硖, 薛静, 张晓东. 植物花青素合成途径中的调控基因研究进展. 生物技术进展, 2011, 1(6): 381-390.
	GONG X, XUE J, ZHANG X D. Regulation genes in plant anthocyanin synthesis pathway. Current Biotechnology, 2011, 1(6): 381-390. (in Chinese)
[9]	ALBERT N W, DAVIES K M, LEWIS D H, ZHANG H B, MONTEFIORI M, BRENDOLISE C, BOASE M R, NGO H, JAMESON P E, SCHWINN K E. A conserved network of transcriptional activators and repressors regulates anthocyanin pigmentation in eudicots. The Plant Cell, 2014, 26(3): 962-980. doi: 10.1105/tpc.113.122069. doi: 10.1105/tpc.113.122069 pmid: 24642943
[10]	李翔, 刘杨, 李怀志, 王中彦, 陈火英. 茄花青素5-O糖基转移酶基因克隆与表达特征分析. 上海交通大学学报(农业科学版), 2011, 29(6): 1-5, 23.
	LI X, LIU Y, LI H Z, WANG Z Y, CHEN H Y. Cloning and expression characterization of the anthocyanin 5-O glucosyltransferase in eggplant. Journal of Shanghai Jiao Tong University (Agricultural Science), 2011, 29(6): 1-5, 23. (in Chinese)
[11]	WANG S J, CHEN Z, JI T, DI Q H, LI L J, WANG X F, WEI M, SHI Q H, LI Y, GONG B, YANG F J. Genome-wide identification and characterization of the R2R3MYB transcription factor superfamily in eggplant (Solanum melongena L.). Agri Gene, 2016, 2: 38-52. doi: 10.1016/j.aggene.2016.09.006 doi: 10.1016/j.aggene.2016.09.006
[12]	STOMMEL J R, DUMM J M. Coordinated regulation of biosynthetic and regulatory genes coincides with anthocyanin accumulation in developing eggplant fruit. Journal of the American Society for Horticultural Science, 2015, 140(2): 129-135. doi: 10.21273/JASHS.140.2.129
[13]	SAKAMURA S, WATANABE S, OBATA Y. The structure of the major anthoeyanin in eggplant. Agricultural and Biological Chemistry, 1963, 27(9): 663-665. doi: 10.1080/00021369.1963.10858161. doi: 10.1080/00021369.1963.10858161
[14]	孙保娟, 李植良, 黎振兴, 罗少波, 徐晓美. 茄子果色上位性基因D的定位及其InDel分子标记开发与应用. 中国, ZL2016 1 1128459.5. 2018-03-02.
	SUN B J, LI Z L, LI Z X, LUO S B, XU X M. Mapping of eggplant fruit color epistatic gene D, and InDel molecular marker development and application. China, ZL2016 1 1128459.5. 2018-03-02. (in Chinese)
[15]	孙保娟, 李植良, 黎振兴, 罗少波, 吴俊. 与茄子果色上位性基因P紧密连锁的InDel分子标记及应用. 中国, ZL201810018063.8. 2018-10-02.
	SUN B J, LI Z L, LI Z X, LUO S B, WU J. InDel molecular marker closely linked with epistatic gene P of eggplant fruit color and application of InDel molecular marker. China, ZL201810018063.8. 2018-10-02. (in Chinese)
[16]	李慧敏. 茄子果色上位D基因SmMYB1克隆及其在茄子花青素合成中的功能分析[D]. 广州: 华南农业大学, 2020.
	LI H M. Cloning of epigenetic D gene SmMYB1 controlling peel color and its function in anthocyanin synthesis in eggplant[D]. Guangzhou: South China Agricultural University, 2020. (in Chinese)
[17]	吴俊. 茄子果色上位基因P基因分子标记开发及候选基因分析[D]. 广州: 华南农业大学, 2020.
	WU J. Molecular marker development and candidate gene analysis of epistasis P gene controlling fruit color in eggplant (Solanum melongena L.)[D]. Guangzhou: South China Agricultural University, 2020. (in Chinese)
[18]	SPRINGOB K, NAKAJIMA J, YAMAZAKI M, SAITO K. Recent advances in the biosynthesis and accumulation of anthocyanins. Natural Product Reports, 2003, 20(3): 288-303. doi: 10.1039/b109542k. doi: 10.1039/ b109542k pmid: 12828368
[19]	GISBERT C, DUMM J M, PROHENS J, VILANOVA S, STOMMEL J R. A spontaneous eggplant (Solanum melongena L.) color mutant conditions anthocyanin-free fruit pigmentation. Hortscience, 2016, 51(7): 793-798. doi: 10.21273/HORTSCI.51.7.793. doi: 10.21273/HORTSCI.51.7.793
[20]	ZHANG Y J, HU Z L, CHU G H, HUANG C, TIAN S B, ZHAO Z P, CHEN G P. Anthocyanin accumulation and molecular analysis of anthocyanin biosynthesis-associated genes in eggplant (Solanum melongena L.). Journal of Agricultural and Food Chemistry, 2014, 62(13): 2906-2912. doi: 10.1021/jf404574c
[21]	邵文婷, 刘杨, 韩洪强, 陈火英. 茄子花青素合成相关基因SmMYB的克隆与表达分析. 园艺学报, 2013, 40(3): 467-478.
	SHAO W T, LIU Y, HAN H Q, CHEN H Y. Cloning and expression analysis of an anthocyanin-related transcription factor gene SmMYB in eggplant. Acta Horticulturae Sinica, 2013, 40(3): 467-478. (in Chinese)
[22]	王海竹, 曲红云, 周婷婷, 徐启江. 茄萼花色苷合成相关基因DFR和MYB克隆及表达分析. 中国农业科学, 2017, 50(14): 2781-2792.
	WANG H Z, QU H Y, ZHOU T T, XU Q J. Cloning and expression analysis of anthocyanin biosynthesis-associated DFR and MYB genes in Calyx of eggplant (Solanum melongena L.). Scientia Agricultura Sinica, 2017, 50(14): 2781-2792. (in Chinese)
[23]	DOCIMO T, FRANCESE G, RUGGIERO A, BATELLI G, DE PALMA M, BASSOLINO L, TOPPINO L, ROTINO G L, MENNELLA G, TUCCI M. Phenylpropanoids accumulation in eggplant fruit: characterization of biosynthetic genes and regulation by a MYB transcription factor. Frontiers in Plant Science, 2015, 6: 1233. doi: 10.3389/fpls.2015.01233. doi: 10.3389/fpls.2015.01233 pmid: 26858726
[24]	DAUNAY M C, AUBERT S, FRARY A, DOGANLAR S, LESTER R N, BARENDSE G, VAN DER WEERDEN G, HENNART J W, HAANSTRA J, DAUPHIN F, JULLIAN E. Eggplant (Solanum melongena) fruit color:pigments, measurements and genetics // Proceeding of the 12 Eucarpia Meeting on Genetics and Breeding of Capsicum and Eggplant, 2004: 108-116.
[25]	AZUMA K, OHYAMA A, IPPOUSHI K, ICHIYANAGI T, TAKEUCHI A, SAITO T, FUKUOKA H. Structures and antioxidant activity of anthocyanins in many accessions of eggplant and its related species. Journal of Agricultural and Food Chemistry, 2008, 56(21): 10154-10159. doi: 10.1021/jf801322m. doi: 10.1021/jf801322m pmid: 18831559
[26]	张映, 赵悦琪, 陈钰辉, 连勇, 刘富中. 茄子紫色形成的分子研究进展. 园艺学报, 2019, 46(9): 1779-1796.
	ZHANG Y, ZHAO Y Q, CHEN Y H, LIAN Y, LIU F Z. Progress in molecular research on purple formation of eggplant. Acta Horticulturae Sinica, 2019, 46(9): 1779-1796. (in Chinese)
[27]	JEONG S T, GOTO-YAMAMOTO N, HSSHIZUME K, ESAKA M. Expression of the flavonoid 3’-hydroxylase and flavonoid 3’5’ hydroxylase genes and flavonoid composition in grape (Vitis vinefera). Plant Science, 2006, 17(1): 61-69. doi: 10.1016/j.plantsci.2005.07.025. doi: 10.1016/j.plantsci.2005.07. 025
[28]	GRAMAZIO P, PROHENS J, PLAZAS M, ANDÚJAR I, HERRAIZ F J, CASTILLO E, KNAPP S, MEYER R S, VILANOVA S. Location of chlorogenic acid biosynthesis pathway and polyphenol oxidase genes in a new interspecific anchored linkage map of eggplant. BMC Plant Biology, 2014, 14: 350. doi: 10.1186/s12870-014-0350-z. doi: 10.1186/s12870-014-0350-z pmid: 25491265
[29]	MENNELLA G, LO SCALZO R, FIBIANI M, D'ALESSANDRO A, FRANCESE G, TOPPINO L, ACCIARRI N, DE ALMEIDA A E, ROTINO G L. Chemical and bioactive quality traits during fruit ripening in eggplant (Solanum melongena L.) and allied species. Journal of Agricultural and Food Chemistry, 2012, 60(47): 11821-11831. doi: 10.1021/jf3037424. doi: 10.1021/jf3037424
[30]	DOCIMO T, FRANCESE G, RUGGIERO A, BATELLI G, DE PALMA M, BASSOLINO L, TOPPINO L, ROTINO G L, MENNELLA G, TUCCI M. Phenylpropanoids accumulation in eggplant fruit: characterization of biosynthetic genes and regulation by a MYB transcription factor. Frontiers in Plant Science, 2015, 6: 1233. doi: 10.3389/fpls.2015.01233. doi: 10.3389/fpls.2015.01233 pmid: 26858726
[31]	PAYYAVULA R S, SHAKYA R, SENGODA V G, MUNYANEZA J E, SWAMY P, NAVARRE D A. Synthesis and regulation of chlorogenic acid in potato: Rerouting phenylpropanoid flux in HQT- silenced lines. Plant Biotechnology Journal, 2015, 13(4): 551-564. doi: 10.1111/pbi.12280. doi: 10.1111/pbi.12280

基因ID Gene ID	基因注释 Gene annotation	E3316_vs_19141 log2倍数变化 Log2 fold change	19141_vs_19147 log2倍数变化 log2 fold change
EGP22200	查尔酮异构酶基因 Chalcone isomerase 1 (CHI1)	-3.00	6.94
EGP24232	查尔酮异构酶基因 Chalcone isomerase 3 (CHI3)	-2.13	3.59
EGP30923	黄烷酮3-羟化酶基因 Flavanone 3-hydroxylase (F3H)	-2.49	2.85
EGP10290	黄酮3′-羟化酶基因 Flavonoid 3′-hydroxylase (F3'H)	_	1.15
EGP32037	黄酮3′,5′-羟化酶基因 Flavonoid 3′5′-hydroxylase (F3′5′H)	-4.50	9.32
EGP31017	二氢黄酮醇4-还原酶 Dihydroflavonol 4-reductase (DFR)	-1.53	6.93
EGP18904	花青素合成酶基因 Anthocyanidin synthase (ANS)	-1.96	7.43
EGP16482	花青素O-甲基转移酶基因 Anthocyanin, O-methyltransferase (OMT)	-4.35	8.26
EGP06309	花青素O-甲基转移酶基因 Anthocyanin, O-methyltransferase (OMT)	_	-1.91
EGP24021	反式肉桂酸4-单加氧酶基因 cinnamate-4-hydroxylase (C4H)	_	3.31
EGP05102	羟基肉桂酰转移酶基因 Shikimate/quinate hydroxycinnamoyl transferase (HCT)	-4.33	2.33
EGP30738	羟基肉桂酰转移酶基因 Shikimate/quinate hydroxycinnamoyl transferase (HCT)	-1.24	1.74
EGP01163	羟基肉桂酰转移酶基因 Shikimate/quinate hydroxycinnamoyl transferase (HCT)	-1.75	_
EGP02613	羟基肉桂酰辅酶A奎尼羟基肉桂转移酶 Hydroxycinnamoyl coenzyme A-quinate transferase (HQT)	-0.98	_
EGP13381	反式肉桂酸4-单加氧酶基因 cinnamate-4-hydroxylase (C4H)	2.20	-1.93
EGP07013	羟基肉桂酰转移酶基因 Shikimate/quinate hydroxycinnamoyl transferase (HCT)	1.59	-0.91
EGP32043	羟基肉桂酰转移酶基因 Shikimate/quinate hydroxycinnamoyl transferase (HCT)	6.12	-7.32
EGP10080	羟基肉桂酰转移酶基因 Shikimate/quinate hydroxycinnamoyl transferase (HCT)	1.16	_

转录组及代谢组联合解析茄子果色上位遗传效应

Transcriptome and Metabolome Integrated Analysis of Epistatic Genetics Effects on Eggplant Peel Color

RichHTML

PDF

赞

可视化

摘要/Abstract

引用本文

使用本文

图/表 8

参考文献 31

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	唐玉林, 张博, 任曼, 张瑞雪, 秦俊杰, 朱浩, 郭延生. UPLC-MS/MS代谢组学评价归芪益母口服液对产后奶牛瘤胃的调节作用[J]. 中国农业科学, 2023, 56(2): 368-378.
[2]	李青林,张文涛,徐慧,孙京京. 低磷胁迫下黄瓜木质部与韧皮部汁液的代谢物变化[J]. 中国农业科学, 2022, 55(8): 1617-1629.
[3]	吕馨宁,王玥,贾润普,王胜男,姚玉新. 不同温度下褪黑素处理对‘阳光玫瑰'葡萄采后品质的影响[J]. 中国农业科学, 2022, 55(7): 1411-1422.
[4]	闫乐乐,卜璐璐,牛良,曾文芳,鲁振华,崔国朝,苗玉乐,潘磊,王志强. 广泛靶向代谢组学解析桃蚜危害对桃树次生代谢产物的影响[J]. 中国农业科学, 2022, 55(6): 1149-1158.
[5]	彭佳堃, 戴伟东, 颜涌泉, 张悦, 陈丹, 董明花, 吕美玲, 林智. 基于代谢组学的‘永春佛手’乌龙茶化学成分解析[J]. 中国农业科学, 2022, 55(4): 769-784.
[6]	陈婷婷, 符卫蒙, 余景, 奉保华, 李光彦, 符冠富, 陶龙兴. 彩色稻叶片光合特征及其与抗氧化酶活性、花青素含量的关系[J]. 中国农业科学, 2022, 55(3): 467-478.
[7]	由玉婉,张雨,孙嘉毅,张蔚. ‘月月粉’月季NAC家族全基因组鉴定及皮刺发育相关成员的筛选[J]. 中国农业科学, 2022, 55(24): 4895-4911.
[8]	尤佳玲,李有梅,孙孟豪,谢兆森. ‘黑比诺’葡萄不同叶龄叶片叶绿体内淀粉积累及其相关基因表达差异分析[J]. 中国农业科学, 2022, 55(21): 4265-4278.
[9]	刘鑫,张亚红,袁苗,党仕卓,周娟. ‘红地球’葡萄花芽分化过程中的转录组分析[J]. 中国农业科学, 2022, 55(20): 4020-4035.
[10]	徐献斌,耿晓月,李慧,孙丽娟,郑焕,陶建敏. 基于转录组分析ABA促进葡萄花青苷积累相关基因[J]. 中国农业科学, 2022, 55(1): 134-151.
[11]	郭永春, 王鹏杰, 金珊, 侯炳豪, 王淑燕, 赵峰, 叶乃兴. 基于WGCNA鉴定茶树响应草甘膦相关的基因共表达模块[J]. 中国农业科学, 2022, 55(1): 152-166.
[12]	袁景丽,郑红丽,梁先利,梅俊,余东亮,孙玉强,柯丽萍. 花青素代谢对陆地棉叶片和纤维色泽呈现的影响[J]. 中国农业科学, 2021, 54(9): 1846-1855.
[13]	陈华枝,范元婵,蒋海宾,王杰,范小雪,祝智威,隆琦,蔡宗兵,郑燕珍,付中民,徐国钧,陈大福,郭睿. 基于纳米孔全长转录组数据完善东方蜜蜂微孢子虫的基因组注释[J]. 中国农业科学, 2021, 54(6): 1288-1300.
[14]	张桂云,朱静雯,孙明法,严国红,刘凯,宛柏杰,代金英,朱国永. 盐胁迫条件下长白10号水稻籽粒中差异代谢物的分析[J]. 中国农业科学, 2021, 54(4): 675-683.
[15]	杜宇,祝智威,王杰,王秀娜,蒋海宾,范元婵,范小雪,陈华枝,隆琦,蔡宗兵,熊翠玲,郑燕珍,付中民,陈大福,郭睿. 利用第三代纳米孔长读段测序技术构建和注释蜜蜂球囊菌的全长转录组[J]. 中国农业科学, 2021, 54(4): 864-876.