基于代谢组和转录组解析多雨寡照对桃果皮着色和类黄酮积累的影响

doi:10.3864/j.issn.0578-1752.2025.06.010

中国农业科学 ›› 2025, Vol. 58 ›› Issue (6): 1173-1194.doi: 10.3864/j.issn.0578-1752.2025.06.010

基于代谢组和转录组解析多雨寡照对桃果皮着色和类黄酮积累的影响

孙萍¹(), 朱文灿²^,³(), 林贤锐¹, 吴嘉颀¹, 曹译文¹, 陈辰斐¹, 王轶¹, 朱建锡¹, 贾惠娟⁴, 钱敏杰²^,³(), 沈建生¹()

¹ 金华市农业科学研究院（浙江省农业机械研究院），浙江金华 321017
² 海南大学三亚南繁研究院，海南三亚 572025
³ 海南大学热带农林学院/海南省热带园艺作物品质调控重点实验室，海口 570228
⁴ 浙江大学农业与生物技术学院，杭州 310013

收稿日期:2024-09-07 接受日期:2024-10-20 出版日期:2025-03-25 发布日期:2025-03-25
通信作者:
钱敏杰，E-mail：minjie.qian@hainanu.edu.cn。
沈建生，E-mail：sjsjhnky@163.com
联系方式: 孙萍，E-mail：sunpingzju@163.com。朱文灿，E-mail：wencanzhu@hainanu.edu.cn。孙萍和朱文灿为同等贡献作者。
基金资助:
浙江省“十四五”农业（果品）新品种选育重大科技专项-桃李新品种选育(2021C02066-4)

Effects of Rainy and Low Light Conditions on Coloration and Flavonoid Accumulation in Peach Peel Based on Metabolomic and Transcriptomic Analyses

SUN Ping¹(), ZHU WenCan²^,³(), LIN XianRui¹, WU JiaQi¹, CAO YiWen¹, CHEN ChenFei¹, WANG Yi¹, ZHU JianXi¹, JIA HuiJuan⁴, QIAN MinJie²^,³(), SHEN JianSheng¹()

¹ Jinhua Academy of Agricultural Sciences (Zhejiang Institute of Agricultural Machinery), Jinhua 321017, Zhejiang
² Sanya Nanfan Research Institute of Hainan University, Sanya 572025, Hainan
³ School of Tropical Agriculture and Forestry, Hainan University/Key Laboratory of Quality Regulation of Tropical Horticultural Crop in Hainan Province, Haikou 570228
⁴ College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310013

Received:2024-09-07 Accepted:2024-10-20 Published:2025-03-25 Online:2025-03-25

摘要/Abstract

摘要：

【目的】桃的果皮色泽与果实的外观品质和经济价值紧密相关，花青苷是桃果皮着色的决定性色素物质。本研究基于代谢组和转录组联合分析，探究梅雨季节寡照多雨的环境条件对桃果皮类黄酮（花青苷、黄酮醇、原花青素）含量和类黄酮合成相关基因表达的影响，鉴定和挖掘桃类黄酮生物合成通路关键基因以及转录调控因子，为改善梅雨季节桃栽培措施、促进桃果皮着色以及进一步丰富桃类黄酮生物合成分子机制提供应用依据和理论基础。【方法】以桃品种‘中金蟠7-12号’为试材。模拟多雨环境栽培为T1试验组，模拟寡照多雨环境栽培为T2试验组以及避雨栽培（模仿正常栽培环境）为CK对照组。在不同时期（0D和24D），对不同处理的桃果实果皮样品进行代谢组测定以及转录组测序（RNA-Seq）分析。进一步通过KEGG富集通路以及加权基因共表达网络分析（WGCNA）鉴定调节类黄酮化合物生物合成的关键候选基因。【结果】代谢组测定结果显示，矢车菊素-3-O-葡萄糖苷、原花青素B1和槲皮素-3-O-葡萄糖苷分别为桃果皮中花青苷、原花青素和黄酮醇的主要成分。其中，矢车菊素-3-O-葡萄糖苷是桃果皮红色着色的决定性物质。T1和T2处理均抑制了桃果皮的花青苷含量，并且以T2处理的抑制效果更显著。通过RNA-Seq分析，共鉴定得到8 296个差异表达基因（DEG），其中24D-T1 vs 0D组间筛选得到的DEG数目最多，为6 879个。通过WGCNA，来自于绿松石、红色、绿黄色、棕色、蓝色和紫红色模块的基因被鉴定为参与调节桃果实果皮中类黄酮化合物生物合成的候选基因。KEGG富集分析显示，代谢途径是除了绿松石模块外，其他所有模块候选基因中被富集到最多的通路。基于WGCNA，鉴定确认了15个类黄酮生物合成通路相关结构基因，此外还鉴定得到了MYB、bHLH、ERF、bZIP以及C2H2等转录因子。【结论】梅雨季节的多雨寡照显著抑制了桃果皮中花青苷的积累以及桃果实果皮的转红，避雨栽培可以在雨季提高桃果实的外观品质和经济价值。此外，鉴定得到一些与类黄酮生物合成密切相关的关键结构基因和调控基因，可为梅雨季节改善桃果实着色提供理论指导。

关键词: 桃, 果皮色泽, 多雨寡照, 类黄酮, 转录组, 代谢组

Abstract:

【Objective】 The color of peach peel is closely related to the appearance quality and economic value of peach fruit, and anthocyanins are the predominant pigment substances for peach peel coloring. This study was based on combined metabolomic and transcriptomic analyses to investigate the effects of low light and rainy conditions during the rainy season on the accumulation of flavonoids (anthocyanins, flavonols, and proanthocyanidins) in peach peel and the transcriptional expression of related biosynthetic genes, identify and excavate key genes and transcriptional factors regulating flavonoids biosynthesis, and to provide application and theoretical basis for improving peach cultivation practices to enhance peach peel coloring during the rainy season, and further enriching the molecular mechanism of peach flavonoid biosynthesis.【Method】 The peach cultivar ‘Zhongjin Pan 7-12’ was used as the material in this study. The imitated rainy condition was regarded as treatment 1 (T1), the imitated low light and rainy conditions were regarded as treatment 2 (T2), and shelter cultivation (imitating normal cultivation environment) was the control (CK) group. Peach fruit peel samples were collected for metabolomic and RNA-Seq analyses at different stages (0D and 24D). Key candidate genes regulating flavonoids biosynthesis were identified through KEGG and weighted gene correlation network analysis (WGCNA).【Result】 The results of metabolomic analysis showed that cyanidin-3-O-glucoside, procyanidin B1, and quercetin-3-O-glucoside were the main components of anthocyanins, procyanidins, and flavonols in peach peel, respectively. Among them, cyanidin-3-O-glucoside was the predominant substance for the red coloration of peach peel. Both T1 and T2 treatments inhibited the anthocyanin accumulation in peach peel, with the more pronounced effect by T2. Through RNA-Seq result analysis, a total of 8 296 differentially expressed genes (DEGs) were identified, among which the highest number of DEGs was obtained through the comparison group of 24D-T1 vs 0D, with 6 879. Through WGCNA, genes from turquoise, red, greenyellow, brown, blue, and magenta modules were identified as candidate genes involved in regulating flavonoid biosynthesis in peach fruit peel. KEGG enrichment analysis showed that metabolic pathways were the most enriched pathway among candidate genes in all modules except for the turquoise module. Based on WGCNA, 15 structural genes related to flavonoid biosynthesis pathway were identified. In addition, transcription factors such as MYB, bHLH, ERF, bZIP, and C2H2 were also identified.【Conclusion】 Rainy and low light conditions significantly inhibit the anthocyanin accumulation and red coloration in peach peel. Shelter cultivation can be used to improve the appearance quality and economic value of peach fruit during the rainy season. In addition, key structural and regulatory genes related to flavonoid biosynthesis were identified, which can provide theoretical guidance for improving peach fruit coloring during the rainy season.

Key words: peach (Prunus persica), peel color, rainy and low light, flavonoid, transcriptome, metabolome

孙萍, 朱文灿, 林贤锐, 吴嘉颀, 曹译文, 陈辰斐, 王轶, 朱建锡, 贾惠娟, 钱敏杰, 沈建生. 基于代谢组和转录组解析多雨寡照对桃果皮着色和类黄酮积累的影响[J]. 中国农业科学, 2025, 58(6): 1173-1194.

SUN Ping, ZHU WenCan, LIN XianRui, WU JiaQi, CAO YiWen, CHEN ChenFei, WANG Yi, ZHU JianXi, JIA HuiJuan, QIAN MinJie, SHEN JianSheng. Effects of Rainy and Low Light Conditions on Coloration and Flavonoid Accumulation in Peach Peel Based on Metabolomic and Transcriptomic Analyses[J]. Scientia Agricultura Sinica, 2025, 58(6): 1173-1194.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2025.06.010

https://www.chinaagrisci.com/CN/Y2025/V58/I6/1173

图/表 10

表1

图1

表2

图2

图3

图4

图5

图6

图7

图8

参考文献 66

[1]	刘冠儒. 湖北省孝感市桃产业现状与发展研究[D]. 武汉: 武汉轻工大学, 2023.
	LIU G R. Research on the status and development of peach industry in Xiaogan City, Hubei Province[D]. Wuhan: Wuhan Polytechnic University, 2023. (in Chinese)
[2]	李晓颖, 谭洪花, 房经贵, 韩键, 宋长年. 果树果实的风味物质及其研究. 植物生理学报, 2011, 47(10): 943-950.
	LI X Y, TAN H H, FANG J G, HAN J, SONG C N. Flavor compounds in fruits and research on them. Plant Physiology Journal, 2011, 47(10): 943-950. (in Chinese)
[3]	HICHRI I, BARRIEU F, BOGS J, KAPPEL C, DELROT S, LAUVERGEAT V. Recent advances in the transcriptional regulation of the flavonoid biosynthetic pathway. Journal of Experimental Botany, 2011, 62(8): 2465-2483. doi: 10.1093/jxb/erq442 pmid: 21278228
[4]	WILLIAMS C A, GRAYER R J. Anthocyanins and other flavonoids. Natural Product Reports, 2004, 21(4): 539-573. doi: 10.1039/b311404j pmid: 15282635
[5]	ONO E, RUIKE M, IWASHITA T, NOMOTO K, FUKUI Y. Co-pigmentation and flavonoid glycosyltransferases in blue Veronica persica flowers. Phytochemistry, 2010, 71(7): 726-735.
[6]	WINKEL-SHIRLEY B. Flavonoid biosynthesis. A colorful model for genetics, biochemistry, cell biology, and biotechnology. Plant Physiology, 2001, 126(2): 485-493.
[7]	LEPINIEC L, DEBEAUJON I, ROUTABOUL J M, BAUDRY A, POURCEL L, NESI N, CABOCHE M. Genetics and biochemistry of seed flavonoids. Annual Review of Plant Biology, 2006, 57: 405-430. pmid: 16669768
[8]	CHAPPLE C C S, SHIRLEY B W, ZOOK M, HAMMERSCHMIDT R, SOMERVILLE S C. 36 Secondary metabolism in Arabidopsis// Cold Spring Harbor Monograph Archive, 1994, 27: 989-1030.
[9]	DEBEAUJON I, NESI N, PEREZ P, DEVIC M, GRANDJEAN O, CABOCHE M, LEPINIEC L. Proanthocyanidin-accumulating cells in Arabidopsis testa: Regulation of differentiation and role in seed development. The Plant Cell, 2003, 15(11): 2514-2531.
[10]	SARMA A D, SHARMA R. Anthocyanin-DNA copigmentation complex: Mutual protection against oxidative damage. Phytochemistry, 1999, 52(7): 1313-1318.
[11]	QIAN M J, ROSENQVIST E, PRINSEN E, PESCHECK F, FLYGARE A M, KALBINA I, JANSEN M A K, STRID Å. Downsizing in plants—UV light induces pronounced morphological changes in the absence of stress. Plant Physiology, 2021, 187(1): 378-395.
[12]	DIXON R A, XIE D Y, SHARMA S B. Proanthocyanidins - a final frontier in flavonoid research? New Phytologist, 2005, 165(1): 9-28.
[13]	KNEKT P, JÄRVINEN R, SEPPÄNEN R, HELLÖVAARA M, TEPPO L, PUKKALA E, AROMAA A. Dietary flavonoids and the risk of lung cancer and other malignant neoplasms. American Journal of Epidemiology, 1997, 146(3): 223-230. doi: 10.1093/oxfordjournals.aje.a009257 pmid: 9247006
[14]	MIDDLETON E, KANDASWAMI C, THEOHARIDES T C. The effects of plant flavonoids on mammalian cells: Implications for inflammation, heart disease, and cancer. Pharmacological Reviews, 2000, 52(4): 673-751. pmid: 11121513
[15]	HARBORNE J B, WILLIAMS C A. Advances in flavonoid research since 1992. Phytochemistry, 2000, 55(6): 481-504. doi: 10.1016/s0031-9422(00)00235-1 pmid: 11130659
[16]	JAAKOLA L. New insights into the regulation of anthocyanin biosynthesis in fruits. Trends in Plant Science, 2013, 18(9): 477-483. doi: 10.1016/j.tplants.2013.06.003 pmid: 23870661
[17]	MATSUI K, UMEMURA Y, OHME-TAKAGI M. AtMYBL2, a protein with a single MYB domain, acts as a negative regulator of anthocyanin biosynthesis in Arabidopsis. The Plant Journal, 2008, 55(6): 954-967.
[18]	TAKOS A M, JAFFÉ F W, JACOB S R, BOGS J, ROBINSON S P, WALKER A R. Light-induced expression of a MYB gene regulates anthocyanin biosynthesis in red apples. Plant Physiology, 2006, 142(3): 1216-1232.
[19]	WANG N, XU H F, JIANG S H, ZHANG Z Y, LU N L, QIU H R, QU C Z, WANG Y C, WU S J, CHEN X S. MYB12 and MYB22 play essential roles in proanthocyanidin and flavonol synthesis in red-fleshed apple (Malus sieversii f. niedzwetzkyana). The Plant Journal, 2017, 90(2): 276-292.
[20]	DIRLEWANGER E, GRAZIANO E, JOOBEUR T, GARRIGA- CALDERÉ F, COSSON P, HOWAD W, ARÚS P. Comparative mapping and marker-assisted selection in Rosaceae fruit crops. Proceedings of the National Academy of Sciences of the United States of America, 2004, 101(26): 9891-9896.
[21]	TUAN P A, BAI S, YAEGAKI H, TAMURA T, HIHARA S, MORIGUCHI T, ODA K. The crucial role of PpMYB10.1 in anthocyanin accumulation in peach and relationships between its allelic type and skin color phenotype. BMC Plant Biology, 2015, 15(1): 280.
[22]	SHI B, WU H X, ZHENG B, QIAN M J, GAO A P, ZHOU K B. Analysis of light-independent anthocyanin accumulation in mango (Mangifera indica L.). Horticulturae, 2021, 7(11): 423.
[23]	ZHU W C, WU H X, YANG C K, SHI B, ZHENG B, MA X W, ZHOU K B, QIAN M J. Postharvest light-induced flavonoids accumulation in mango (Mangifera indica L.) peel is associated with the up-regulation of flavonoids-related and light signal pathway genes. Frontiers in Plant Science, 2023, 14: 1136281.
[24]	LI Y Y, MAO K, ZHAO C, ZHAO X Y, ZHANG H L, SHU H R, HAO Y J. MdCOP1 ubiquitin E3 ligases interact with MdMYB1 to regulate light-induced anthocyanin biosynthesis and red fruit coloration in apple. Plant Physiology, 2012, 160(2): 1011-1022.
[25]	孟宪宸, 崔彦志, 李星宇, 张秀玲, 马振伟, 柴菊华. 成熟期降水对碣石山产区‘马瑟兰’果实品质的影响. 中外葡萄与葡萄酒, 2023(3): 24-31.
	MENG X C, CUI Y Z, LI X Y, ZHANG X L, MA Z W, CHAI J H. Effect of precipitation during mature period on fruit quality of ‘Marselan’ in Jieshishan area. Sino-Overseas Grapevine & Wine, 2023(3): 24-31. (in Chinese)
[26]	姜琳琳, 王静, 张晓煜, 陈仁伟, 胡宏远. 成熟期降水对贺兰山东麓酿酒葡萄果实品质的影响. 中国农业气象, 2020, 41(3): 156-161.
	JIANG L L, WANG J, ZHANG X Y, CHEN R W, HU H Y. Rainfall effect of rainfall on the quality of wine grape during the ripening stage at the east foot of Helan Mountain. Chinese Journal of Agrometeorology, 2020, 41(3): 156-161. (in Chinese)
[27]	沈玉英, 熊彩珍, 贾惠娟. 盖棚对湖景蜜露桃着果及果实品质的影响. 中国南方果树, 2006(5): 67-68.
	SHEN Y Y, XIONG C Z, JIA H J. The effect of canopy construction on the fruiting and fruit quality of ‘Hujingmilu’ peach. South China Fruits, 2006(5): 67-68. (in Chinese)
[28]	李玲, 王慧, 张梅, 陈修德, 高东升. 设施栽培桃果实芳香成分的GC-MS分析. 山东农业大学学报(自然科学版), 2011, 42(1): 41-48.
	LI L, WANG H, ZHANG M, CHEN X D, GAO D S. GC-MS analysis on fruit aroma components of peach in protected culture. Journal of Shandong Agricultural University (Natural Science Edition), 2011, 42(1): 41-48. (in Chinese)
[29]	JIA H J, ARAKI A, OKAMOTO G. Influence of fruit bagging on aroma volatiles and skin coloration of ‘Hakuho’ peach (Prunus persica Batsch). Postharvest Biology and Technology, 2005, 35(1): 61-68.
[30]	成梦园. 不同避雨膜对鲜食葡萄生长发育和果实品质的影响研究[D]. 太谷: 山西农业大学, 2022.
	CHENG M Y. Effects of rainproof film on growth and development and fruit quality of fresh grape[D]. Taigu: Shanxi Agricultural University, 2022. (in Chinese)
[31]	MENG J F, NING P F, XU T F, ZHANG Z W. Effect of rain-shelter cultivation of Vitis vinifera cv. Cabernet Gernischet on the phenolic profile of berry skins and the incidence of grape diseases. Molecules, 2013, 18(1): 381-397.
[32]	CLAIRE D, WATTERS N, GENDRON L, BOILY C, PÉPIN S, CARON J. High productivity of soilless strawberry cultivation under rain shelters. Scientia Horticulturae, 2018, 232: 127-138.
[33]	LIM K H, GU M, SONG J H, CHO Y S, KIM W S, KIM B S, JUNG S K, CHOI H S. Growth, fruit production, and disease occurrence of rain-sheltered Asian pear trees. Scientia Horticulturae, 2014, 177: 37-42.
[34]	熊彩珍, 凌柏芳, 李洁, 景筱荣. 避雨设施栽培对桃果实生长发育及糖分的影响. 浙江林业科技, 2012, 32(3): 46-49.
	XIONG C Z, LING B F, LI J, JING X R. Study on fruit growth and sugar degree under rain shelter cultivation of Amygdalus persica. Journal of Zhejiang Forestry Science and Technology, 2012, 32(3): 46-49. (in Chinese)
[35]	VERDE I, JENKINS J, DONDINI L, MICALI S, PAGLIARANI G, VENDRAMIN E, PARIS R, ARAMINI V, GAZZA L, ROSSINI L, et al. The peach v2.0 release: High-resolution linkage mapping and deep resequencing improve chromosome-scale assembly and contiguity. BMC Genomics, 2017, 18(1): 225.
[36]	徐长宝, 陈国强. 江淮梅雨对梨树生长结果及果实品质的影响. 中国南方果树, 2007(6): 74-75.
	XU C B, CHEN G Q. The effect of Jianghuai Meiyu on the growth, fruiting and fruit quality of pear trees. South China Fruits, 2007(6): 74-75. (in Chinese)
[37]	马艳儿, 杨丽, 何玉云, 李华, 王华. 降水对葡萄多酚类物质的影响. 江苏农业科学, 2016, 44(5): 221-224.
	MA Y E, YANG L, HE Y Y, LI H, WANG H. The effect of precipitation on grape polyphenols. Jiangsu Agricultural Sciences, 2016, 44(5): 221-224. (in Chinese)
[38]	FENG F J, LI M J, MA F W, CHENG L L. Phenylpropanoid metabolites and expression of key genes involved in anthocyanin biosynthesis in the shaded peel of apple fruit in response to sun exposure. Plant Physiology and Biochemistry, 2013, 69: 54-61.
[39]	WEI Y Z, HU F C, HU G B, LI X J, HUANG X M, WANG H C. Differential expression of anthocyanin biosynthetic genes in relation to anthocyanin accumulation in the pericarp of Litchi chinensis Sonn. PLoS ONE, 2011, 6(4): e19455.
[40]	李舒婷, 李小溪, 高媛, 潘秋红. 简易避雨栽培对‘赤霞珠’葡萄果实类黄酮物质表征的影响. 果树学报, 2018, 35(7): 802-816.
	LI S T, LI X X, GAO Y, PAN Q H. Effect of simple rain-shelter cultivation on flavonoid profiling in Vitis vinifera ‘Cabernet Sauvignon’ grape berries. Journal of Fruit Science, 2018, 35(7): 802-816. (in Chinese)
[41]	曹锰, 郭景南, 魏志峰, 高登涛, 程大伟, 孙晓文. 避雨栽培对‘金手指’葡萄果实生长及香气物质组分的影响. 果树学报, 2015, 32(5): 894-902.
	CAO M, GUO J N, WEI Z F, GAO D T, CHENG D W, SUN X W. Effects of rain-shelter cultivation on development and aromatic component of ‘Gold Finger’ grape. Journal of Fruit Science, 2015, 32(5): 894-902. (in Chinese)
[42]	BLANKE M, BALMER M. Cultivation of sweet cherry under rain covers. Acta Horticulturae, 2008, 795: 479-484.
[43]	杨乐, 李文杨, 赵师成, 孙耀清, 申洁梅. 避雨栽培措施对信阳五月鲜桃果实生长的影响. 经济林研究, 2019, 37(1): 205-210.
	YANG L, LI W Y, ZHAO S C, SUN Y Q, SHEN J M. Effects of rain-shelter cultivation measures on fruit growth in Xinyang Wuyuexian peach. Non-Wood Forest Research, 2019, 37(1): 205-210. (in Chinese)
[44]	薄流齐洋. 避雨栽培对刺葡萄生长发育及果实品质的影响[D]. 长沙: 湖南农业大学, 2021.
	BO L Q Y. Influence of rain-shielding cultivation on growth and development and fruit quality of Vitis davidii Foex[D]. Changsha: Hunan Agricultural University, 2021. (in Chinese)
[45]	刘亚妮. 避雨栽培对葡萄生长发育及果实品质的影响[D]. 秦皇岛: 河北科技师范学院, 2019.
	LIU Y N. Effects of rain-shelter cultivation on grape growth and fruit quality[D]. Qinhuangdao: Hebei Normal University of Science & Technology, 2019. (in Chinese)
[46]	赵云. 两品种桃果皮花青苷积累差异及光照调控机制[D]. 杭州: 浙江大学, 2019.
	ZHAO Y. Mechanisms for differences accumulation of anthocyanin in peel of two peach cultivars and the regulation by light[D]. Hangzhou: Zhejiang University, 2019. (in Chinese)
[47]	HWANG K H, MIN B H, SHIN H, AHN B J. Petal color changes in carnation plants transformed with an antisense DFR and a CHI gene. HortScience, 2005, 40(4): 1051.
[48]	JU Z G, LIU C L, YUAN Y B. Activities of chalcone synthase and UDPGal: Flavonoid-3-o-glycosyltransferase in relation to anthocyanin synthesis in apple. Scientia Horticulturae, 1995, 63(3): 175-185.
[49]	BOGS J, DOWNEY M O, HARVEY J S, ASHTON A R, TANNER G J, ROBINSON S P. Proanthocyanidin synthesis and expression of genes encoding leucoanthocyanidin reductase and anthocyanidin reductase in developing grape berries and grapevine leaves. Plant Physiology, 2005, 139(2): 652-663. doi: 10.1104/pp.105.064238 pmid: 16169968
[50]	LIAO L, VIMOLMANGKANG S, WEI G C, ZHOU H, KORBAN S S, HAN Y P. Molecular characterization of genes encoding leucoanthocyanidin reductase involved in proanthocyanidin biosynthesis in apple. Frontiers in Plant Science, 2015, 6: 243. doi: 10.3389/fpls.2015.00243 pmid: 25914714
[51]	HAN Y, VIMOLMANGKANG S, SORIA-GUERRA R E, KORBAN S S. Introduction of apple ANR genes into tobacco inhibits expression of both CHI and DFR genes in flowers, leading to loss of anthocyanin. Journal of Experimental Botany, 2012, 63(7): 2437-2447.
[52]	BAUDRY A, HEIM M A, DUBREUCQ B, CABOCHE M, WEISSHAAR B, LEPINIEC L. TT2, TT8, and TTG1 synergistically specify the expression of BANYULS and proanthocyanidin biosynthesis in Arabidopsis thaliana. The Plant Journal, 2004, 39(3): 366-380.
[53]	HEPPEL S C, JAFFÉ F W, TAKOS A M, SCHELLMANN S, RAUSCH T, WALKER A R, BOGS J. Identification of key amino acids for the evolution of promoter target specificity of anthocyanin and proanthocyanidin regulating MYB factors. Plant Molecular Biology, 2013, 82(4): 457-471.
[54]	KARPPINEN K, LAFFERTY D J, ALBERT N W, MIKKOLA N, MCGHIE T, ALLAN A C, AFZAL B M, HÄGGMAN H, ESPLEY R V, JAAKOLA L. MYBA and MYBPA transcription factors co-regulate anthocyanin biosynthesis in blue-coloured berries. New Phytologist, 2021, 232(3): 1350-1367. doi: 10.1111/nph.17669 pmid: 34351627
[55]	JIANG L Y, YUE M L, LIU Y Q, ZHANG N T, LIN Y X, ZHANG Y T, WANG Y, LI M Y, LUO Y, ZHANG Y, WANG X R, CHEN Q, TANG H R. A novel R2R3-MYB transcription factor FaMYB5 positively regulates anthocyanin and proanthocyanidin biosynthesis in cultivated strawberries (Fragaria × ananassa). Plant Biotechnology Journal, 2023, 21(6): 1140-1158. doi: 10.1111/pbi.14024 pmid: 36752420
[56]	TAO R Y, YU W J, GAO Y H, NI J B, YIN L, ZHANG X, LI H X, WANG D S, BAI S L, TENG Y W. Light-induced basic/helix- loop-helix64 enhances anthocyanin biosynthesis and undergoes CONSTITUTIVELY PHOTOMORPHOGENIC1-mediated degradation in pear. Plant Physiology, 2020, 184(4): 1684-1701.
[57]	ZHOU H, LIN-WANG K, LIAO L, GU C, LU Z Q, ALLAN A C, HAN Y P. Peach MYB7 activates transcription of the proanthocyanidin pathway gene encoding leucoanthocyanidin reductase, but not anthocyanidin reductase. Frontiers in Plant Science, 2015, 6: 908. doi: 10.3389/fpls.2015.00908 pmid: 26579158
[58]	YAO G F, MING M L, ALLAN A C, GU C, LI L T, WU X, WANG R Z, CHANG Y J, QI K J, ZHANG S L, WU J. Map-based cloning of the pear gene MYB114identifies an interaction with other transcription factors to coordinately regulate fruit anthocyanin biosynthesis. The Plant Journal, 2017, 92(3): 437-451.
[59]	NI J B, BAI S L, ZHAO Y, QIAN M J, TAO R Y, YIN L, GAO L, TENG Y W. Ethylene response factors Pp4ERF24 and Pp12ERF96 regulate blue light-induced anthocyanin biosynthesis in ‘Red Zaosu’ pear fruits by interacting with MYB114. Plant Molecular Biology, 2019, 99(1/2): 67-78.
[60]	WANG S, ZHANG X H, LI B B, ZHAO X Q, SHEN Y, YUAN Z H. Genome-wide identification and characterization of bZIP gene family and cloning of candidate genes for anthocyanin biosynthesis in pomegranate (Punica granatum). BMC Plant Biology, 2022, 22(1): 170.
[61]	ZHOU P, LI J W, JIANG H Y, JIN Q J, WANG Y J, XU Y C. Analysis of bZIP gene family in lotus (Nelumbo) and functional study of NnbZIP36in regulating anthocyanin synthesis. BMC Plant Biology, 2023, 23(1): 429.
[62]	WANG D R, YANG K, WANG X, LIN X L, RUI L, LIU H F, LIU D D, YOU C X. Overexpression of MdZAT5, an C2H2-type zinc finger protein, regulates anthocyanin accumulation and salt stress response in apple calli and Arabidopsis. International Journal of Molecular Sciences, 2022, 23(3): 1897.
[63]	LIU W J, WANG Y C, YU L, JIANG H Y, GUO Z W, XU H F, JIANG S H, FANG H C, ZHANG J, SU M Y, ZHANG Z Y, CHEN X L, CHEN X S, WANG N. MdWRKY 11 participates in anthocyanin accumulation in red-fleshed apples by affecting MYB transcription factors and the photoresponse factor MdHY5. Journal of Agricultural and Food Chemistry, 2019, 67(32): 8783-8793.
[64]	ZHANG J K, WANG Y C, MAO Z L, LIU W N, DING L C, ZHANG X N, YANG Y W, WU S Q, CHEN X S, WANG Y L. Transcription factor McWRKY71 induced by ozone stress regulates anthocyanin and proanthocyanidin biosynthesis in Malus crabapple. Ecotoxicology and Environmental Safety, 2022, 232: 113274.
[65]	SUN Q G, JIANG S H, ZHANG T L, XU H F, FANG H C, ZHANG J, SU M Y, WANG Y C, ZHANG Z Y, WANG N, CHEN X S. Apple NAC transcription factor MdNAC52 regulates biosynthesis of anthocyanin and proanthocyanidin through MdMYB9 and MdMYB11. Plant Science, 2019, 289: 110286.
[66]	ZHOU H, LIN-WANG K, WANG H L, GU C, DARE A P, ESPLEY R V, HE H P, ALLAN A C, HAN Y P. Molecular genetics of blood-fleshed peach reveals activation of anthocyanin biosynthesis by NAC transcription factors. The Plant Journal, 2015, 82(1): 105-121. doi: 10.1111/tpj.12792 pmid: 25688923

基因编号Gene ID	正向引物Forward primer (5′-3′)	反向引物Reverse primer (5′-3′)
Prupe.7G168300_v2.0.a1	GGACTGGACACAGAGGCATT	GGGGCATTTTGGGTAGAAAT
Prupe.1G376400_v2.0.a1	GGGGTGCTAGACATCCTCAA	CTGGTGCTCTTCGACATTCA
Prupe.4G200500_v2.0.a1	TGAAGGGCTGTGATGGTGTA	TCCAAGGTTCCTTTGATTGC
Prupe.2G324700_v2.0.a1	TGCCTCTCCCAACACTCTCT	CCATCAGCCACATCAAACAC
Prupe.2G212900_v2.0.a1	TCTGGGGAGCAGAAGAAGAA	CAGGCATTGCATAGGGTTTT
Prupe.8G125100_v2.0.a1	ACGCCCTATTGACACAGTCC	CTTGGGCCCCTTTTTGTTAT
Prupe.7G225600_v2.0.a1	TGTCTCAGCCGTGAAAGATG	TGCAGAAGCAGAAGCAGAAA
Prupe.3G240000_v2.0.a1	AAGGGTGGACTCGTTTTGTG	TCTTCTCCTCCCCTCTCGAT
β-actin	GATTCCGGTGCCCAGAAGT	CCAGCAGCTTCCATTCCAA

样品 Sample	原始数据 Raw reads	过滤后数据 <BOLD>C</BOLD>lean reads	过滤后碱基总数 Clean base (G)	测序错误率 Error rate (%)	碱基质量值 Q20 (%)	碱基质量值 Q30 (%)	GC含量 GC content (%)
0D-1	46947136	45207546	6.78	0.03	97.83	93.78	46.90
0D-2	45181526	43587342	6.54	0.02	98.16	94.61	46.71
0D-3	43048874	41413698	6.21	0.02	98.12	94.44	46.82
24D-T1-1	45453162	43941034	6.59	0.02	98.12	94.49	46.88
24D-T1-2	43462974	42019444	6.30	0.02	98.16	94.56	46.90
24D-T1-3	51322012	49684760	7.45	0.02	98.07	94.34	47.14
24D-T2-1	46961188	45358530	6.80	0.02	98.14	94.50	46.56
24D-T2-2	51255062	49645836	7.45	0.02	98.04	94.26	46.88
24D-T2-3	50412392	48881676	7.33	0.02	98.16	94.57	46.82
24D-CK-1	47734934	46239584	6.94	0.03	97.99	94.12	46.58
24D-CK-2	52248104	50503542	7.58	0.02	98.15	94.52	46.88
24D-CK-3	56671330	54632786	8.19	0.03	98.02	94.22	47.14

基于代谢组和转录组解析多雨寡照对桃果皮着色和类黄酮积累的影响

Effects of Rainy and Low Light Conditions on Coloration and Flavonoid Accumulation in Peach Peel Based on Metabolomic and Transcriptomic Analyses

RichHTML

PDF

赞

可视化

摘要/Abstract

引用本文

使用本文

图/表 10

参考文献 66

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	邹晓威, 夏蕾, 朱晓敏, 孙辉, 周琦, 齐霁, 张亚封, 郑岩, 姜兆远. 基于转录组测序的玉米瘤黑粉菌UM01240过表达菌株诱导玉米抗病性分析[J]. 中国农业科学, 2025, 58(6): 1116-1130.
[2]	谢露露, 李福, 张思远, 高建昌. 基于跨物种转录组解析影响不定根发生的保守基因[J]. 中国农业科学, 2025, 58(6): 1195-1209.
[3]	高岩浩, 王婷婷, 白卫卫, 杜兴杰, 刘贤, 秦本源, 付彤, 孙宇, 高腾云, 张天留. 脂质组与转录组联合揭示南阳牛不同肌肉组织脂质特征的差异表达模式[J]. 中国农业科学, 2025, 58(6): 1239-1258.
[4]	郭奥琳, 林俊璇, 赖恭梯, 贺丽媛, 车建美, 潘若, 杨方学, 黄玉吉, 陈桂信, 赖呈纯. 过表达VdF3′5′H2对刺葡萄细胞花青素组分积累的影响[J]. 中国农业科学, 2025, 58(4): 802-818.
[5]	罗朝丹, 冯春梅, 李建强, 黎新荣, 韦勇, 杨李益, 刘筱瑾, 谭和, 任二芳, 罗小杰. 基于GC-MS非靶向代谢组学分析不同成熟度‘台农一号’芒果香气物质[J]. 中国农业科学, 2025, 58(3): 564-581.
[6]	潘媛, 王德, 刘楠, 孟祥龙, 戴蓬博, 李波, 胡同乐, 王树桐, 曹克强, 王亚南. 两种高通量测序技术鉴定苹果病毒效果评价及两种新病毒的鉴定[J]. 中国农业科学, 2025, 58(2): 266-280.
[7]	郭天发, 吴金龙, 仇倩倩, 马新超, 王力荣, 吴翠云. 新疆土桃果皮纯色形成与PpMYB10.1启动子变异的关系[J]. 中国农业科学, 2025, 58(2): 326-338.
[8]	曹言勇, 程泽强, 马娟, 杨文博, 朱卫红, 孙新艳, 李慧敏, 夏来坤, 段灿星. 整合转录组和代谢组学分析揭示玉米对层出镰孢茎腐病的响应机制[J]. 中国农业科学, 2025, 58(1): 75-90.
[9]	郭磊, 黄晨艳, 宋宏峰, 沈志军, 张斌斌, 马瑞娟, 孙朦, 何鑫, 俞明亮. 桃苗圃适用除草剂的筛选、混配与安全性评价[J]. 中国农业科学, 2024, 57(9): 1734-1747.
[10]	戚仁洁, 宁宇, 刘静, 刘之洋, 徐海, 罗志丹, 陈龙正. 基于转录组测序的苦瓜皂苷合成相关基因的鉴定和分析[J]. 中国农业科学, 2024, 57(9): 1779-1793.
[11]	林蔚, 吴水金, 李跃森. 转录组和蛋白质组关联分析解析巴西蕉幼苗响应低温的分子机制[J]. 中国农业科学, 2024, 57(8): 1575-1591.
[12]	肖刘华, 康乃慧, 李树成, 郑致远, 罗绕绕, 陈金印, 陈明, 向妙莲. 茉莉酸甲酯对猕猴桃果实抗葡萄座腔菌过程中能量代谢和膜脂代谢的影响[J]. 中国农业科学, 2024, 57(7): 1377-1393.
[13]	安秀红, 孙妍, 王芳, 冯启科, 王宁, 李津津, 张俊佩, 王红霞. 河北省太行山区‘辽宁1号’核桃叶片营养诊断技术研究[J]. 中国农业科学, 2024, 57(6): 1153-1166.
[14]	高晨曦, 郝陆洋, 胡悦, 李永祥, 张登峰, 李春辉, 宋燕春, 石云素, 王天宇, 黎裕, 刘旭洋. 干旱条件下玉米转座子插入关联的表观调控分析[J]. 中国农业科学, 2024, 57(6): 1034-1048.
[15]	熊尚烨, 张翔, 梁宝慧, 叶仰东, 李浴阳, 朱晓, 朱志鸿, 官华忠, 张帅, 吴建国, 胡杰. 水稻抗褐飞虱基因QBPH1和QBPH4的精细定位与聚合效应分析[J]. 中国农业科学, 2024, 57(23): 4619-4631.