





中国农业科学 ›› 2020, Vol. 53 ›› Issue (6): 1224-1233.doi: 10.3864/j.issn.0578-1752.2020.06.014
所属专题: 专题——土壤活性有机碳
李亚林1,张旭博2(
),任凤玲1,孙楠1(
),徐梦2,徐明岗1
收稿日期:2019-06-11
接受日期:2019-08-05
出版日期:2020-03-16
发布日期:2020-04-09
联系方式:
李亚林,Tel:15238579116;E-mail:henanyalin@163.com。
基金资助:
YaLin LI1,XuBo ZHANG2(
),FengLing REN1,Nan SUN1(
),Meng XU2,MingGang XU1
Received:2019-06-11
Accepted:2019-08-05
Published:2020-03-16
Online:2020-04-09
摘要:
【目的】施肥是影响农田土壤溶解性有机碳、氮的重要因子。探讨在不同利用方式、熟制、土壤pH等条件下长期施肥对土壤溶解性有机碳(DOC)、溶解性有机氮(DON)含量的影响,为农田土壤碳氮管理提供指导。【方法】收集2000—2019年已发表文献72篇,获得相对独立数据(510组DOC和208组DON),采用整合分析(Meta-analysis)方法定量分析不同利用方式、熟制和土壤pH下施肥对DOC和DON含量的影响。【结果】与不施肥相比,施肥均能显著提高土壤DOC和DON含量,其中施有机肥(单施或配施)的提高幅度(60%和93%)是化肥(13%和29%)的4.6倍和3.2倍。不同利用方式下,施肥能显著提高旱地土壤DOC和DON含量,且旱地施用有机肥提升土壤DOC和DON的幅度显著高于水旱轮作。不同熟制比较,一年一熟下施用有机肥后DOC含量提高85 %,显著高于一年两熟(38%);不同pH土壤比较,碱性土壤(pH>7.5)上施用有机肥后DOC和DON含量分别提高了85%和162%,显著高于6.5<pH<7.5的中性土壤(48%和70%)和pH<6.5 的酸性土壤(32%和61%)。【结论】施用有机肥(单施或配施)可显著提高DOC和DON含量,但其效果会因利用方式、熟制、土壤pH等的不同有较大差异,因此,有机肥的施用应综合考虑相应的土壤和环境条件。
李亚林,张旭博,任凤玲,孙楠,徐梦,徐明岗. 长期施肥对中国农田土壤溶解性有机碳氮含量影响的整合分析[J]. 中国农业科学, 2020, 53(6): 1224-1233.
YaLin LI,XuBo ZHANG,FengLing REN,Nan SUN,Meng XU,MingGang XU. A Meta-Analysis of Long-Term Fertilization Impact on Soil Dissolved Organic Carbon and Nitrogen Across Chinese Cropland[J]. Scientia Agricultura Sinica, 2020, 53(6): 1224-1233.
表1
不同施肥处理下DOC和DON含量"
| 项目 Item | 土壤溶解性有机碳DOC | 土壤溶解性有机氮DON | ||||
|---|---|---|---|---|---|---|
| 不施肥CK | 化肥 NPK | 有机肥 OM | 不施肥CK | 化肥 NPK | 有机肥 OM | |
| 样本数 No. | 96 | 166 | 245 | 44 | 61 | 103 |
| 平均值 Mean | 90.81b | 97.19b | 141.34a | 12.36b | 16.70b | 24.39a |
| 中值 Median | 51.83 | 60.91 | 105.57 | 10.10 | 14.00 | 19.80 |
| 偏度 Skewness | 2.10 | 1.27 | 1.16 | 1.80 | 1.86 | 1.56 |
| 峰度 Kurtosis | 5.99 | 1.02 | 0.76 | 3.70 | 4.56 | 2.32 |
| 范围 Range | 4.64—548.06 | 6.38—385.83 | 6.83—552.70 | 0.94—52.12 | 1.22—76.74 | 1.83—99.32 |
表2
不同利用方式、熟制和土壤pH下同一施肥方式对土壤溶解性有机碳氮含量提升幅度(%)的差异性比较"
| 项目 Items | 土壤溶解性有机碳 DOC | 土壤溶解性有机氮 DON | |||||
|---|---|---|---|---|---|---|---|
| 不施肥 CK | 化肥 NPK | 有机肥 OM | 不施肥 CK | 化肥 NPK | 有机肥 OM | ||
| 利用方式 Use type | 水田 Paddy | 23b | 7b | 34b | 61ab | 25a | 84ab |
| 水旱轮作 Upland-Paddy | 26b | 8ab | 39b | 35b | 11a | 52b | |
| 旱地 Upland | 49a | 18a | 76a | 92a | 39a | 136a | |
| 熟制 Cropping system | 一年一熟 Mono-cropping | 56a | 17a | 85a | 57a | 23a | 124a |
| 一年两熟 Double-cropping | 25b | 10a | 38b | 52a | 36a | 87a | |
| 一年三熟 Triple-cropping | 36ab | 7a | 56ab | 30a | 4a | 55a | |
| 土壤pH Soil pH | pH<6.5 | 23b | 10b | 32c | 47b | 25a | 61b |
| 6.5<pH<7.5 | 30b | 11ab | 48b | 40b | 8a | 70b | |
| pH>7.5 | 55a | 19a | 85a | 111a | 43a | 162a | |
| [1] | KALBITZ K, KAISER K . Contribution of dissolved organic matter to carbon storage in forest mineral soils. Journal of Plant Nutrition and Soil Science, 2008,171(1):52-60. |
| [2] | BURFORD J R, BREMNER J M . Relationships between the denitrification capacities of soils and total, water-soluble and readily decomposable soil organic matter. Soil Biology & Biochemistry, 1975,7(6):389-394. |
| [3] | SACHSE A, BABENZIEN D, GINZEL G, GELBRECHT J, STEINBERG C E W . Characterization of dissolved organic carbon (doc) in a dystrophic lake and an adjacent fen. Biogeochemistry, 2001,54(3):279-296. |
| [4] | 周碧青, 陈成榕, 杨文浩, 张黎明, 邢世和 . 茶树对溶解性有机和无机态氮的吸收与运转特性. 植物营养与肥料学报, 2017,23(1):189-195. |
| ZHOU B Q, CHEN C R, YANG W H, ZHANG L M, XING S H . Absorption and operation characteristics of soluble organic and inorganic nitrogen in tea plant. Journal of Plant Nutrition and Fertilizers, 2017,23(1):189-195. (in Chinese) | |
| [5] | 潘根兴, 曹建华 . 土壤碳及其在地球表层系统碳循环中的意义. 第四纪研究, 2000(4):325-334. |
| PAN G X, CAO J H . Soil carbon and its significance in the carbon cycle of the earth's surface system. Quaternary Sciences, 2000(4):325-334. (in Chinese) | |
| [6] | 张迪, 韩晓增 . 长期不同植被覆盖和施肥管理对黑土活性有机碳的影响. 中国农业科学, 2010,43(13):2715-2723. |
| ZHANG D, HAN X Z . Effects of long-term different vegetation cover and fertilization management on the active organic carbon of black soil. Scientia Agricultura Sinica, 2010,43(13):2715-2723. (in Chinese) | |
| [7] | 徐明岗, 于荣, 王伯仁 . 长期不同施肥下红壤活性有机质与碳库管理指数变化. 土壤学报, 2006,43(5):723-729. |
| XU M G, YU R, WANG B R . Changes of index of active organic matter and carbon pool management in red soil under different fertilization for a long time. Acta Pedologica Sinica, 2006,43(5):723-729. (in Chinese) | |
| [8] | 杨静, 聂三安, 杨文浩, 陈成榕, 张黎明, 周碧青, 邢世和 . 不同施肥水稻土溶解性有机氮组分差异及影响因素. 土壤学报, 2018,55(4):955-966. |
| YANG J, NIE S A, YANG W H, CHENG C R, ZHANG L M, ZHOU B Q, XING S H . The difference of soluble organic nitrogen in rice soil under different fertilization and its influencing factors. Acta Pedologica Sinica, 2018,55(4):955-966. (in Chinese) | |
| [9] | 常单娜, 曹卫东, 包兴国, 白金顺, 张久东, 卢秉林, 高嵩涓, 曾闹华, 王雪翠, 志水胜好 . 西北灌漠土长期不同施肥改变土壤溶解性有机质的化学及光谱学特性. 光谱学与光谱分析, 2016,36(1):220-225. |
| CHANG D N, CAO W D, BAO X G, BAI J S, ZHANG J D, LU B L, GAO S J, ZENG N H, WANG X C, ZHI S S H . The chemical and spectral characteristics of soil soluble organic matter were changed by different fertilization in the desert soils in northwest China. Spectroscopy and Spectral Analysis, 2016,36(1):220-225. (in Chinese) | |
| [10] | 高忠霞, 周建斌, 王祥, 陈兴丽, 杨学云 . 不同培肥处理对土壤溶解性有机碳含量及特性的影响. 土壤学报, 2010,47(1):115-121. |
| GAO Z X, ZHOU J B, WANG X, CEHN X L, YANG X Y . Effects of different fertilizer treatments on soil soluble organic carbon content and properties. Acta Pedologica Sinica, 2010,47(1):115-121. (in Chinese) | |
| [11] | 卢萍, 单玉华, 杨林章, 韩勇 . 秸秆还田对稻田土壤溶液中溶解性有机质的影响. 土壤学报, 2006(5):736-741. |
| LU P, SHAN Y H, YANG L Z, HAN Y . Effects of straw mulching on dissolved organic matter in paddy soil solution. Acta Pedologica Sinica, 2006(5):736-741. (in Chinese) | |
| [12] | BARTON L, WAN G Y, COLMER T D . Turfgrass ( Cynodon dactylon L.) sod production on sandy soils: II. Effects of irrigation and fertiliser regimes on N leaching. Plant and Soil, 2006,284(1/2):147-164. |
| [13] | 张璐, 张文菊, 徐明岗, 蔡泽江, 彭畅, 王伯仁, 刘骅 . 长期施肥对中国3种典型农田土壤活性有机碳库变化的影响. 中国农业科学, 2009,42(5):1646-1655. |
| ZHANG L, ZHANG W J, XU M G, CAI Z J, PENG C, WANG B R, LIU Y . Effects of long-term fertilization on change of labile organic carbon in three typical upland soils of China. Scientia Agricultura Sinica, 2009,42(5):1646-1655. (in Chinese) | |
| [14] | 梁尧, 韩晓增, 宋春, 李海波 . 不同有机物料还田对东北黑土活性有机碳的影响. 中国农业科学, 2011,44(17):3565-3574. |
| LIANG Y, HAN X Z, SONG C, LI H B . Effects of different organic materials on the active organic carbon of northeast black soil. Scientia Agricultura Sinica, 2011,44(17):3565-3574. (in Chinese) | |
| [15] | VESTGARDEN L S, ABRAHAMSEN G, STUANES A O . Soil solution response to nitrogen and magnesium application in a scots pine forest. Soil Science Society of America Journal, 2001,65(6):1812-1823. |
| [16] | GLASS G V . Primary, secondary, and Meta-Analysis of research. Educational Researcher, 1976,5(10):3-8. |
| [17] | AKIYAMA H, YAN X, YAGI K . Evaluation of effectiveness of enhanced-efficiency fertilizers as mitigation options for N2O and NO emissions from agricultural soils: meta-analysis. Global Change Biology, 2010,16(6):1837-1846. |
| [18] | REN F L, ZHANG X B, LIU J, SUN N, SUN Z G, WU L H, XU M G . A synthetic analysis of livestock manure substitution effects on organic carbon changes in China's arable topsoil. Catena, 2018,171:1-10. |
| [19] | SHAN J, YAN X Y . Effects of crop residue returning on nitrous oxide emissions in agricultural soils. Atmospheric Environment, 2013,71(Complete):170-175. |
| [20] | LUO Y Q, HUI D F, ZHANG D Q . Elevated CO2 stimulates net accumulations of carbon and nitrogen in land ecosystems: A Meta-analysis. Ecology, 2006,87(1):53-63. |
| [21] | GEISSELER D, SCOW K M . Long-term effects of mineral fertilizers on soil microorganisms-A review. Soil Biology and Biochemistry, 2014, 75:54-63. |
| [22] | HEDGES L V, CURTIS G P S . The Meta-Analysis of response ratios in experimental ecology. Ecology, 1999, 80(4):1150-1156. |
| [23] | CURTIS P S, WANG X . A Meta-Analysis of elevated CO2 effects on woody plant mass, form, and physiology. Oecologia (Berlin), 1998,113(3):299-313. |
| [24] | LIU C, LU M, CUI J, LI B, FANG C M . Effects of straw carbon input on carbon dynamics in agricultural soils: A meta-analysis. Global Change Biology, 2014,20(5):1366-1381. |
| [25] | 王若琦, 秦超英 . Meta分析中异质性检验方法的改进. 科学技术与工程, 2012,12(10):12-15. |
| WANG R Q, QIN C Y . Improvement of heterogeneity test in meta-analysis. Science Technology and Engineering, 2012,12(10). (in Chinese) | |
| [26] | 邵兴芳, 徐明岗, 张文菊, 黄敏, 周显, 朱平, 高洪军 . 长期有机培肥模式下黑土碳与氮变化及氮素矿化特征. 植物营养与肥料学报, 2014,20(2):326-335. |
| SHAO X F, XU M G, ZHANG W J, HUANG M, ZHOU X, ZHU P, GAO H J . Carbon and nitrogen changes and mineralization characteristics of black soil under long-term organic fertilization. Journal of Plant Nutrition and Fertilizers,2014, 20(2):326-335. (in Chinese) | |
| [27] | 杨玉爱, 王珂, 叶正钱, 魏幼璋 . 有机肥料资源及其对微量元素的螯溶和利用的研究. 土壤通报, 1994(S1):21-25, 7. |
| YANG Y A, WANG K, YE Z Q, WEI Y Z . Study on organic fertilizer resources and its chelation and utilization of trace elements. Chinese Journal of Soil Science, 1994(S1):21-25, 7. (in Chinese) | |
| [28] | 赵满兴, 周建斌, 陈竹君, 杨绒 . 有机肥中溶解性有机碳、氮含量及其特性. 生态学报, 2007(1):397-403. |
| ZHAO M X, ZHOU J B, CHEN Z J, YANG R . Contents and characteristics of soluble organic carbon and nitrogen in organic fertilizers. Acta Ecologica Sinica, 2007,27(1):397-403. (in Chinese) | |
| [29] | 徐华勤, 肖润林, 邹冬生, 宋同清, 罗文, 李盛华 . 长期施肥对茶园土壤微生物群落功能多样性的影响. 生态学报, 2007(8):3355-3361. |
| XU H Q, XIAO R L, ZHOU D S, SONG T Q, LUO W, LI S H . Effects of long-term fertilization on the functional diversity of soil microbial communities in tea plantations. Acta Ecologica Sinica, 2007(8):3356-3361. (in Chinese) | |
| [30] | 杜相革, 董民, 曲再红, 史咏竹 . 有机农业和土壤生物多样性. 中国农学通报, 2004(4):80-81, 83. |
| DU X G, DONG M, QU Z H, SHI Y Z . Organic agriculture and soil biodiversity. Chinese Agricultural Science Bulletin, 2004(4):80-81, 83. (in Chinese) | |
| [31] | JACKSON L E, CALDERON F J, STEENWERTN K L, SCOW K M, ROLSTON D E . Responses of soil microbial processes and community structure to tillage events and implications for soil quality. Geoderma, 2003,114(3):305-317. |
| [32] | 朱兆良 . 中国土壤的氮素肥力与农业中的氮素管理//沈善敏.中国土壤肥力. 北京: 中国农业出版社 1998: 160-211. |
| ZHU Z L. Nitrogen fertility in Chinese soil and nitrogen management in agriculture//SHEN S M.Soil Fertility in China. Beijing: China Agriculture Press, 1998: 160-211. (in Chinese) | |
| [33] | 陈玉真, 王峰, 尤志明, 张文锦, 翁伯琦 . 不同类型茶园土壤氮素矿化特性及其与土壤性质的关系. 福建农业学报, 2015,30(7):679-684. |
| CHEN Y Z, WANG F, YOU Z M, ZHANG W J, WENG B Q . Mineralization characteristics of nitrogen and its relationship with soil properties in different types of tea plantations. Fujian Journal of Agricultural Sciences, 2015,30(7):679-684. (in Chinese) | |
| [34] | MICHALZIK B, KALBITZ K, PARK J H, SOLINGER S, MATZNER E . Fluxes and concentrations of dissolved organic carbon and nitrogen-a synthesis for temperate forests. Biogeochemistry, 2001,52(2):173-205. |
| [35] | KAISER K, GUGGENBERGER G . The role of DOM sorption to mineral surfaces in the preservation of organic matter in soils. Organic Geochemistry, 2000,31(7):711-725. |
| [36] | 王桂跃, 苏婷, 韩海亮, 谭禾平, 包斐, 赵福成 . 长期施肥对水田和旱地土壤微生物群落结构、活性碳氮及酶活性的影响. 浙江农业学报, 2018,30(5):817-824. |
| WANG G Y, SU T, HAN H L, TAN H P, BAO F, ZHAO F C . Effects of long-term fertilization on microbial community structure, activated carbon and nitrogen and enzyme activities in paddy field and upland soil. Acta Agriculturae Zhejiangensis, 2018,30(5):817-824. (in Chinese) | |
| [37] | LUNDQUIST E J, JACKSON L E, SCOW K M, HSU C . Changes in microbial biomass and community composition, and soil carbon and nitrogen pools after incorporation of rye into three California agricultural soils. Soil Biology & Biochemistry, 1999,31(2):221-236. |
| [38] | LUNDQUIST E J, JACKSON L E, SCOW K M . Wet-dry cycles affect dissolved organic carbon in two California agricultural soils. Soil Biology and Biochemistry, 1999,31:1031-1038. |
| [39] | 尧水红 . 干湿交替强度对旱地土壤结构形成及水稻秸秆分解过程的相互作用的影响[D]. 南京: 南京农业大学, 2005. |
| YAO S H . Effects of alternate intensities of dry and wet on the formation of soil structure in upland and the interaction of straw decomposition in rice[D]. Nanjing: Nanjing Agricultural University, 2005. (in Chinese) | |
| [40] | 李廷强, 杨肖娥 . 土壤中水溶性有机质及其对重金属化学与生物行为的影响. 应用生态学报, 2004(6):1083-1087. |
| LI T Q, YANG X E . Water-soluble organic matter in soil and its effect on chemical and biological behavior of heavy metals. Chinese Journal of Applied Ecology, 2004(6):1083-1087. (in Chinese) | |
| [41] | TIPPING E, HURLEY M A . A model of solid-solution interactions in acid organic soils, based on the complexation properties of humic substances. European Journal of Soil Science, 1988,39(4):505-519. |
| [42] | TIPPING E, WOOF C . Humic substances in acid organic soils: modelling their release to the soil solution in terms of humic charge. European Journal of Soil Science, 1990,41(4):573-586. |
| [43] | KAISER K, KAUPENJOHANN M . Salts and acids as determinants of the solution composition of acidic forest soils-Anion effects. Journal of Plant Nutrition and Soil Science, 1998,161:105-120. |
| [44] | ERICH M S, TRUSTY G M . Chemical characterization of dissolved organic matter released by limed and unlimed forest soil horizons. Canadian Journal of Soil Science, 1997,77(3):405-413. |
| [45] | CURTIN D, CAMPBELL C A, JALIL A . Effects of acidity on mineralization: pH-dependence of organic matter mineralization in weakly acidic soils. Soil Biology and Biochemistry, 1998,30(1):57-64. |
| [46] | 孙凤霞, 张伟华, 徐明岗, 张文菊, 李兆强, 张敬业 . 长期施肥对红壤微生物生物量碳氮和微生物碳源利用的影响. 应用生态学报, 2010,21(11):2792-2798. |
| SUN F X, ZHANG W H, XU M G, ZHANG W J, LI Z Q, ZHANG J Y . Effects of long-term fertilization on microbial biomass carbon and nitrogen and microbial carbon source utilization in red soil. Chinese Journal of Applied Ecology, 2010,21(11):2792-2798. (in Chinese) |
| [1] | 李星宇, 黄容, 鲜一鸣, 田娇娇, 马晓瑾, 杨乔茜, 李冰, 王昌全. 稻田土壤不同粒级团聚体有机碳组分及CO2排放对长期施肥的响应[J]. 中国农业科学, 2026, 59(6): 1255-1271. |
| [2] | 魏圆慧, 余益辉, 李子钧, 丁文杰, 涂文龙, 毛艳玲. 长期施肥对黄泥田土壤有机碳化学结构与固碳细菌群落结构的影响[J]. 中国农业科学, 2026, 59(5): 1020-1033. |
| [3] | 温玉斌, 白珊珊, 蔡泽江, 孙楠, 徐明岗. 有机物料对土壤微生物量的影响及其酸度调节机理[J]. 中国农业科学, 2026, 59(4): 834-849. |
| [4] | 王仁卓, 李玥颖, 黄绍敏, 姜桂英, 张琦, 刘超麟, 杨锦, 王梦茹, 王贝贝, 刘芳, 郭斗斗, 介晓磊, 宋练, 刘世亮. 长期有机无机肥配施对潮土细菌群落结构、生态网络及关键物种的影响[J]. 中国农业科学, 2026, 59(2): 354-367. |
| [5] | 郭慧婷, 孙艳稳, 牛毅恒, 李亚鹏, 李建华, 徐明岗. 施肥显著改变中国北方农田土壤细菌多样性和优势菌群——Meta分析[J]. 中国农业科学, 2026, 59(1): 114-128. |
| [6] | 白雨欣, 刘灵芝, 安婷婷, 李双异, 汪景宽. 长期不同施肥对棕壤细菌群落结构和碳代谢功能的影响[J]. 中国农业科学, 2025, 58(8): 1579-1590. |
| [7] | 吴欣珈, 薛玮, 严翊丹, 聂莹莹, 叶立明, 徐丽君. 呼伦贝尔土壤有机碳时空变异特征及其影响因素[J]. 中国农业科学, 2025, 58(6): 1145-1158. |
| [8] | 佘文婷, 孙蕊卿, 党海燕, 李文虎, 张丰, 田怡, 徐隽峰, 丁玉兰, 王朝辉. 我国主要麦区农户小麦籽粒硫的含量、分布及影响因素[J]. 中国农业科学, 2025, 58(5): 956-974. |
| [9] | 孙蕊卿, 党海燕, 佘文婷, 王星舒, 褚宏欣, 王涛, 丁玉兰, 罗一诺, 徐隽峰, 李小涵, 王朝辉. 影响我国主要麦区小麦籽粒和面粉锌含量的产量构成要素与土壤肥力因素[J]. 中国农业科学, 2025, 58(2): 291-306. |
| [10] | 袁慧琳, 李雅莹, 顾文杰, 徐培智, 卢钰升, 孙丽丽, 周昌敏, 黎婉玲, 仇荣亮. 长期施用新鲜与腐熟畜禽粪肥土壤溶解性有机质与微生物群落的特征及其关联性[J]. 中国农业科学, 2025, 58(2): 307-325. |
| [11] | 郭斗斗, 张珂珂, 黄绍敏, 宋晓, 张水清, 岳克, 丁世杰, 郭腾飞. 长期不同施肥对潮土磷吸附解吸特性的影响[J]. 中国农业科学, 2025, 58(14): 2805-2820. |
| [12] | 路鹏, 冯佳, 李莉, 杨莉, 杨雷, 吕德智, 薛彦飞. 长期施肥小麦根际与非根际土壤的磷组分及其有效性[J]. 中国农业科学, 2025, 58(12): 2382-2396. |
| [13] | 陈武荣, 肖霜霜, 肖峻, 陈丹. 喀斯特峰丛洼地利用方式对土壤有机碳及其活性组分的影响[J]. 中国农业科学, 2025, 58(10): 1969-1981. |
| [14] | 赵凯男, 丁豪, 刘阿康, 姜宗昊, 陈广周, 冯波, 王宗帅, 李华伟, 司纪升, 张宾, 毕香君, 李勇, 李升东, 王法宏. 氮肥减量后移改善植株光合特性提高麦-玉周年产量及经济效益[J]. 中国农业科学, 2024, 57(5): 868-884. |
| [15] | 胡丹丹, 宋惠洁, 段英华, 吴艳, 胡志华, 徐小林, 张文菊, 何小林, 柳开楼, 苏鹏, 黄群招. 长期施肥对红壤双季稻系统氮素盈亏和土壤碱解氮的影响[J]. 中国农业科学, 2024, 57(24): 4907-4918. |
|
||