中国农业科学

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免疫抑制素和卵泡抑素阻止夏季热应激导致的猪精液品质下降的研究

施振旦;李明;刘丽;黎敏义;秦清明   

  1. 华南农业大学动物科学学院,广州 510642
  • 收稿日期:2010-08-06 出版日期:2011-04-02 发布日期:2010-08-27
  • 作者简介:施振旦,E-mail:zdshi@scau.edu.cn
  • 基金资助:
    国家“863”计划项目(2008AA101003)、国家科技支撑计划项目(2008BADB2B09)

Immunization Against Inhibin/Follistatin to Maintain Normal Semen Quality in Boars Under Summer Heat Stress

SHI Zhen-dan, LI Ming, LIU Li, LI Min-yi, QIN Qing-ming   

  1. College of Animal Sciences, South China Agricultural University, Guangzhou 510642
  • Received:2010-08-06 Online:2011-04-02 Published:2010-08-27

摘要: 【目的】通过免疫抑制素和卵泡抑素促进精子发生,在夏季热应激状态下保持公猪精液品质。【方法】在夏季热应激状态下对精液品质出现下降的成年种公猪单独免疫抑制素、或联合免疫抑制素α亚基和卵泡抑素重组蛋白(n=6)3次,每次间隔21 d;然后在9周试验期间定期采血测定血液抗体效价、FSH和睾酮浓度,每周采精观察精子活力和精子密度作为精液品质指标。【结果】抑制素单独免疫、抑制素和卵泡抑素联合免疫都使猪血液中抗抑制素的抗体效价显著高于对照组值(P<0.01),联合免疫也使猪血液中抗卵泡抑素的抗体效价显著高于对照组值(P<0.01)。单独免疫或联合免疫都显著升高了血液FSH浓度;联合免疫还升高了血液睾酮水平。在试验期间对照组中猪的精子活力持续下降,而两免疫组的精子活力都上升,其中联合免疫猪的精子活力显著高于对照组猪。试验开始时对照组猪精子密度显著高于两免疫组,但在试验期间持续下降;而免疫组中猪精子密度在试验期间未下降,特辑是在联合免疫组于试验后期还显著高于对照组猪。【结论】免疫抑制素和卵泡抑素可以促进公猪FSH和睾酮分泌,克服夏季热应激的不良影响,促进猪精子发生,并提高精液精子活力和密度,有望用于热应激状态下保持种用公猪的繁殖性能。

关键词: 抑制素α亚基 , 卵泡抑素 , 免疫 , 公猪 , 激素 , 精液品质

Abstract: 【Objective】 This study aimed to maintain semen quality and insemination capacity, which were impaired in boars adversely affected by summer heat stress, by immunization against inhibin (IHN) and follistatin (FST). 【Method】 Twenty-one boars, which exhibited semen quality problems of decreased sperm motility in summer months, were selected and equally divided into 3 groups (n=6). Immunization procedures consisted of a primary injection (i.m.) of 2 mg recombinant IHN? α-subunit protein in 2 ml mineral oil adjuvant, and two booster immunizations with 1 mg IHN protein at 21-d intervals for Group A boars. Group B boars were similarly immunized as in Group A boars, but apart from IHN immunogen, also immunized against equal amount of FST protein on each occasion. Group C served as controls that received mineral oil adjuvant on the same occasions. In the 9-wk experimental period, blood samples were collected for measurements of antibody titres, plasma concentrations of FSH and testosterone. Semen was collected every week and examined for sperm motility and density. 【Result】 Immunization against IHN and FST significantly elevated plasma anti-IHN and anti-FST antibody titres, increased FSH and testosterone concentrations, maintained semen sperm motility and sperm density, which in the control boars decreased progressively during the experiment. 【Conclusion】Immunization against IHN and FST was capable of overcoming the adverse impact of heat stress, thus to increase FSH and testosterone secretions, and to maintain boar semen motility, density and reproductivity in hot summer months.

Key words: inhibin &alpha, subunit , follistatin , immunization , boar , hormones , semen quality