家蚕一种hAT家族转座酶基因的分析

doi:10.3864/j.issn.0578-1752.2011.07.022

中国农业科学

家蚕一种hAT家族转座酶基因的分析

沈小绢1，孙霞1，汪生鹏2，郭锡杰2

1、江苏科技大学生物与环境工程学院，江苏镇江212018；
2、中国农业科学院蚕业研究所农业部蚕桑遗传改良重点开放实验室，江苏镇江212018

收稿日期:2010-07-05 出版日期:2011-04-02 发布日期:2010-09-30
通讯作者: 汪生鹏，Tel：0511-85601051；E-mail：spwangsri@163.com
作者简介:沈小绢，Tel：0511-85601051；E-mail：shenxiaojuan158@126.com
基金资助:
国家重点基础性研究发展性计划“973项目”（2005CB121004）、江苏省研究生培养创新工程项目（CX10S-002Z）

Analysis of a hAT Family Transposase Gene in Bombyx mori

SHEN Xiao-juan1, SUN Xia1, WANG Sheng-peng2, GUO Xi-jie2

1、College of Biotechnology and Environmental Engineering, Jiangsu University of Science and Technology, Zhenjiang 212018, Jiangsu;
2、The Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture, Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, Jiangsu

Received:2010-07-05 Online:2011-04-02 Published:2010-09-30

摘要/Abstract

摘要： 【目的】对家蚕中一个潜在的hAT家族转座酶编码基因BmhAT进行分析。【方法】通过生物信息学方法，分析BmhAT转座酶的结构域及序列同源性。采用Real-time PCR方法，对BmhAT在野蚕及家蚕不同品种中的拷贝数进行分析以及在mRNA水平上测定BmhAT在家蚕不同组织中的相对表达量。【结果】BmhAT与许多hAT家族转座酶在序列上有很高的同源性，并具有hAT家族转座酶的保守结构域；BmhAT在家蚕基因组中存在约3—6个拷贝；在家蚕后部丝腺和脂肪体中的表达量分别是参照基因actin 3的0.4倍和0.6倍。【结论】BmhAT可能是家蚕中一个有活性的转座酶基因，编码家蚕一种新的hAT家族转座酶。

关键词: 家蚕 , hAT家族转座子 , 转座酶

Abstract: 【Objective】The objective of this study is to analyze a latent hAT transposase gene of Bombyx mori, named as BmhAT. 【Method】Sequence homology and structural domain of BmhAT were studied using bioinformatical method, the copy number of BmhAT in Bombyx mandarin and different varieties of Bombyx mori were measured by Real-time PCR, and the relative expression of BmhAT at mRNA level in different tissues of Bombyx mori was analyzed by Real-time RT-PCR.【Result】Bioinformatical analysis revealed that BmhAT had significant homology to some hAT family transposase in sequence and contains conserved domains of this family transposase. Real-time PCR results showed that BmhAT had about 3-6 copies in Bombyx mori genome. Quantitative real-time RT-PCR results indicated that the relative expression of BmhAT to actin3 reference gene in posterior silk gland and fat body was 0.4 folds and 0.6 folds, respectively.【Conclusion】BmhAT may be an active transposase gene in Bombyx mori, encoding a new hAT family transposase of Bombyx mori.

Key words: Bombyx mori , hAT family transposon , transposase

沈小绢1，孙霞1，汪生鹏2，郭锡杰2. 家蚕一种hAT家族转座酶基因的分析[J]. 中国农业科学, 2011, 44(7): 1481-1487.

SHEN Xiao-juan1, SUN Xia1, WANG Sheng-peng2, GUO Xi-jie2 . Analysis of a hAT Family Transposase Gene in Bombyx mori[J]. Scientia Agricultura Sinica, 2011, 44(7): 1481-1487.

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家蚕一种hAT家族转座酶基因的分析

Analysis of a hAT Family Transposase Gene in Bombyx mori

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