鸭发育早期下丘脑-垂体生长轴相关基因mRNA的表达特异性分析

doi:10.3864/j.issn.0578-1752.2013.17.021

摘要/Abstract

摘要： 【目的】选择生长速度不同的高邮鸭和金定鸭为试验模型，3种不同品种鸭胚胎期和出雏早期下丘脑垂体生长轴相关基因的表达规律及其与体重和肝重的相关性。【方法】采用实时荧光定量PCR方法研究鸭13、17、21、25、27胚龄和出雏后7日龄下丘脑生长激素释放激素（growth hormone release hormone，GHRH）与生长抑素（somatostatin，SS）、垂体生长激素（growth hormone，GH）和肝脏生长激素受体（growth hormone receptor，GHR）与胰岛素样生长因子（insulin-like growth factors，IGFs） mRNA的表达规律。【结果】证实鸭下丘脑GHRH与SS、垂体GH和肝脏GHR与IGF-I在13胚龄已有表达；除了SS在鸭发育早期维持一个低表达状态外，其它的4种基因均呈现极显著的品种和时间特异性；除了GH外，发现鸭早期发育过程中GHRH、GHR和IGF-I mRNA的表达受到品种和日龄的交互作用的极显著影响。与鸡以往的研究相比，禽类胚胎期下丘脑垂体生长轴相关基因mRNA的表达存在着种属差异。本试验发现鸭早期发育过程中，体重和肝重的变化也呈现极显著的品种和时间特异性，且与下丘脑垂体相关基因的表达呈现不同程度的线性相关。【结论】结果提示下丘脑垂体生长轴基因 mRNA的表达可能在鸭早期发育过程中发挥着重要作用，遗传背景的差异可以导致鸭发育早期体重的差异生长和相关基因的差异表达。

关键词: 鸭 , 早期发育 , 下丘脑垂体生长轴 , mRNA表达 , 体重 , 肝重

Abstract: 【Objective】The present experiment was conducted to study the tissue expression characteristics of related genes mRNA expression in duck hypothalamus-pituitary growth axis during embryogenesis and post-hatching development in different breeds of ducks. 【Method】We have compared the ontogeny of body weight and expression of growth hormone release hormone (GHRH), somatostatin (SS), growth hormone (GH), growth hormone receptor (GHR), insulin-like growth factor-I (IGF-I) mRNA on the embryonic day 13, 17, 21, 25, and 27 and the 7-day-old Gaoyou duck and Jinding duck. 【Result】At the first time, the trial presented that duck hypothalamic GHRH and SS, pituitary GH, liver GHR and IGF- I mRNA were detected at E13. Except SS maintained a lower expression status in the duck early development, the other four genes showed a extremely significant difference in variety and time. In addition to GH, duck GHRH, GHR and IGF-I mRNA expression were significantly affected by breed and age interaction during early development. Compared with previous studies on chicken, significant species differences were observed in poultry embryonic hypothalamus-pituitary growth axis related gene mRNA expression. During early development, extremely significant variety and time specificity was found in developmental change of body and liver weight, and varying degrees of linear correlation was showed between that and related genes mRNA expression in duck hypothalamus-pituitary growth axis. 【Conclusion】It was suggested that different genetic backgrounds may lead to the differential growth rate and the related gene differential expression, and the differential expression of related genes mRNA in duck hypothalamus-pituitary growth axis may be involved in the early growth of ducks.

Key words: duck , early development , hypothalamus-pituitary growth axis , genes mRNA expression , body weight , liver weight

胡艳, 徐文娟, 刘宏祥, 宋卫涛, 宋迟, 陶志云, 单艳菊, 李慧芳. 鸭发育早期下丘脑-垂体生长轴相关基因mRNA的表达特异性分析[J]. 中国农业科学, 2013, 46(17): 3712-3720.

HU Yan, XU Wen-Juan, LIU Hong-Xiang, SONG Wei-Tao, SONG Chi, TAO Zhi-Yun, DAN Yan-Ju, LI Hui-Fang. The Profiles of Related Genes mRNA Expression in Duck Hypothalamus-Pituitary Growth Axis During Early Development[J]. Scientia Agricultura Sinica, 2013, 46(17): 3712-3720.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2013.17.021

https://www.chinaagrisci.com/CN/Y2013/V46/I17/3712

参考文献

[1]Lu F Z, Wang X X, Pan Q X, Huang R H, Liu H L. Expression of genes involved in the somatotropic, thyrotropic, and corticotropic axes during development of Langshan and Arbor Acres chickens. Poultry Science, 2008, 87(10):2087-2097.

[2]Geris K L, Berghman L R, Kuhn E R, Darras V M. Pre- and posthatch developmental changes in hypothalamic thyrotropin-releasing hormone and somatostatin concentrations and in circulating growth hormone and thyrotropin levels in the chicken. The Journal of Endocrinology, 1998, 159(2):219-225.

[3]Porter T E, Couger G S, Dean C E, Hargis B. Ontogeny of growth hormone (GH)-secreting cells during chicken embryonic development: initial somatotrophs are responsive to GH-releasing hormone. Endocrinology, 1995, 136(5):1850-1856.

[4]沈广, 宫桂芬, 吕淑艳, 张晶鑫, 王忠强, 王宁. 我国水禽业的发展状况及趋势展望. 水禽世界, 2009(增刊): 3-17.

Shen G, Gong G F, Lv S Y, Zhang J X, Wang Z Q, Wang N. Industry development status of waterfowl in China. Waterfowl World, 2009(Suppl): 3-17. (in Chinese)

[5]吴荣富. 推进水禽产业集聚的路径研究. 中国畜牧杂志, 2009, 45 (24) : 28-32.

Wu R F. Promote industrial agglomeration Path of waterfowl. Chinese Journal of Animal Science, 2009, 45(24): 28-32. (in Chinese)

[6]Kim H, Barton E, Muja N, Yakar S, Pennisi P, Leroith D. Intact insulin and insulin-like growth factor-I receptor signaling is required for growth hormone effects on skeletal muscle growth and function in vivo. Endocrinology, 2005, 146(4):1772-1779.

[7]赵茹茜, 李四桂. 禽类生长轴的发育及其对生长的调节. 畜牧与兽医, 1999, 31(4) : 35-37.

Zhao R Q, Li S G. Poultry development and its impact on growth axis growth regulation. Husbandry and Veterinary Medicine, 1999, 31(4): 35-37. (in Chinese)

[8]McFarland D C. Influence of growth factors on poultry myogenic satellite cells. Poultry Science, 1999, 78(5):747-758.

[9]赵茹茜, 李四桂, 陈伟华, 雷治海, 陈杰. 胚胎及新生期蛋鸡和肉鸡垂体生长激素基因表达的比较研究. 畜牧兽医学报, 2001, 32(1):5-10.

Zhao R Q, Li S G, Chen W H, Lei Z H, Chen J. A comparative study of pituitary GH gene expression between broiler and layer chickens furing embryonic and neonatal development. Acta Veterinaria et Zootechnica Sinica, 2001, 32(1):5-10. (in Chinese)

[10]Porter T E. Differences in embryonic growth hormone secretion between slow and fast growing chickenstrains. Growth Hormone & IGF Research, 1998, 8(2):133-139.

[11]Kikuchi K, Buonomo F C, Kajimoto Y, Rotwein P. Expression of insulin-like growth factor-I during chicken development. Endocrinology, 1991, 128(3):1323-1328.

[12]Papkoff H, Hayashida T. Pituitary growth hormone from the turtle and duck: purification and immunochemical studies. Proceedings of the Society for Experimental Biology and Medicine, 1972, 140(1): 251-255.

[13]Chen H T, Pan F M, Chang W C. Purification of duck growth hormone and cloning of the complementary DNA. Biochimica et Biophysica Acta, 1988, 949(2):247-251.

[14]Chang M T, Cheng Y S, Huang M C. The SNP genotypes of growth hormone gene associated with reproductive traits in Tsaiya ducks. Reproduction in Domestic Animals, 2012, 47(4):568-573.

[15]Wu Y, Pan A L, Pi J S, Pu Y J, Du J P, Liang Z H, Shen J. One novel SNP of growth hormone gene and its associations with growth and carcass traits in ducks. Molecular Biology Report, 2012, 39(8): 8027-8033.

[16]许盛海, 包文斌, 黄军, 程金花, 束婧婷, 陈国宏. 鸭生长激素基因内含子2、3多态性分析. 遗传, 2007, 29(4):438-442.

Xu S H, Bao W B, Huang J, Cheng J H, Shu J T, Chen G. H. Polymorphic analysis of intron 2 and 3 of growth hormone gene in duck. Hereditas, 2007, 29(4):438-442. (in Chinese)

[17]Zhao R, Wang Y, Zhou Y, Ni Y, Lu L, Grossmann R, Chen J. Dietary daidzein influences laying performance of ducks (Anas platyrhynchos) and early post-hatch growth of their hatchlings by modulating gene expression. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 2004, 138(4):459-466.

[18]葛盛芳, 赵茹茜, 陈伟华, 陈杰. 绍兴蛋鸭和高邮鸭胚胎发育过程中激素水平的变化. 南京农业大学学报, 2000, 23(1): 63-66.

Ge S F, Zhao R Q, Chen W H, Chen J. Changes of some serum hormone levels during embryonic development in Shaoxing and Gaoyou ducks. Journal of Nanjing Agricultural University, 2000, 23(1): 63-66. (in Chinese)

[19]景栋林, 张绮琼, 岑丽莎, 谢蝶娟, 梁晓妮. 鸭胚胎及其部分内脏器官生长发育的研究. 江苏农业科学, 2007, 1: 122-124.

King D L, Zhang Q Q, Cen L S, Xie D J, Liang X N. Study of embryonic growth and development of organs in Duck. Jiangsu Agricultural Sciences, 2007, 1: 122-124. (in Chinese)

[20]Karcher D M, McMurtry J P, Applegate T J. Developmental changes in amniotic and allantoic fluid insulin-like growth factor (IGF)-I and -II concentrations of avian embryos. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 2005, 142(4): 404-409.

[21]Karcher D M, Fleming-Waddell J N, Applegate T J. Developmental changes in insulin-like growth factor (IGF)-I and -II mRNA abundance in extra-embryonic membranes and small intestine of avian embryos. Growth Hormone & IGF Research, 2009, 19(1):31-42.

[22]Wang C Y, Wang Y, Li J, Leung F C. Expression profiles of growth hormone-releasing hormone and growth hormone-releasing hormone receptor during chicken embryonic pituitary development. Poultry Science, 2006, 85(3):569-576.

[23]Burnside J, Cogburn L A. Developmental expression of hepatic growth hormone receptor and insulin-like growth factor-I mRNA in the chicken. Molecular and Cell Endocrinology, 1992, 89(1-2):91-96.

[24]De P F, Robics H L, Caldes T, Alemany J, Scavo L, Serrano J. Insulin-like growth factor-I and insulin as growth and differentiation factors in chicken embryogenesis. Poultry Science, 1991, 70(8):1790-1796.

[25]聂庆华, 张细权, 杨关福. 鸡生长轴相关基因研究进展. 农业生物技术学报, 2003, 11(3):305-312.

Nie Q H, Zhang X Q, Yang G F. Progress of the research on chicken growth axis-related genes. Journal of Agricultural Biotechnology, 2003, 11(3):305-312. (in Chinese)

[26]Harvey S, Johnson C D, Sanders E J. Growth hormone in neural tissues of the chick embryo. The Journal of Endocrinology, 2001, 169(3):487-498.

[27]Darras V M, Finne M F, Berghman L R, Kuhn E R. Ontogeny of the sensitivity of the somatotrophes to thyrotrophin releasing hormone (TRH) and growth hormone releasing factor (GRF) in the embryonic and posthatch chick. Annales d‘Endocrinologie, 1994, 55(6):255-260.