关键词: 大豆幼苗根系, 干旱胁迫, 基因芯片, 实时荧光定量PCR

Abstract:

【Objective】 The objective of this experiment is to study the mechanism of drought resistance in soybean seedling roots. 【Method】 The gene expression of drought-tolerant soybean roots was analyzed under different times of drought stress by cDNA microarray chip. 【Result】 The expression profile of 61 171 genes was obtained. Great changes took place in soybean roots under drought stress and the number of induced genes was more than that of repressed genes. The differential expressions of four genes were confirmed by real-time quantitative PCR and the results indicated that the expression changes of these genes were generally consistent with the results of gene chip. The clustering analysis of hybridization was also carried out to indicate gene expression under drought stress. 【Conclusion】 Expression profiles of different drought-stress times were constructed in order to establish dynamic expression profiles, by which the mutual mechanism between drought tolerance and soybean is entirely acquired, genes responding to drought stress were obtained and gene expression of drought tolerance is completely revealed.

Key words: soybean root, drought stress, cDNA microarray chip, real-time quantitative PCR