2, 4-表油菜素内酯对苹果果实采后贮藏性能的影响

doi:10.3864/j.issn.0578-1752.2026.07.012

中国农业科学 ›› 2026, Vol. 59 ›› Issue (7): 1536-1551.doi: 10.3864/j.issn.0578-1752.2026.07.012

2, 4-表油菜素内酯对苹果果实采后贮藏性能的影响

苏一帆¹(), 杨瞻旭¹, 王迪², 冒俊呈¹, 魏萌萌¹, 陈泽³, 白欣冉³, 楚天歌³, 马昌宁¹, 乔明菲¹, 孙权¹^,⁴^,⁵^,^*(), 胡大刚¹^,^*()

¹ 山东农业大学园艺科学与工程学院/国家苹果工程技术研究中心/农业农村部黄淮地区园艺作物生物学与种质创制重点实验室/山东省果蔬种质创新与利用重点实验室，山东泰安 271018
² 平邑县检验检测中心，山东平邑 273300
³ 山东农业大学未来技术学院，山东泰安 271018
⁴ 华中农业大学果蔬园艺作物种质创新与利用全国重点实验室，武汉 430070
⁵ 湖北洪山实验室，武汉 430070

收稿日期:2025-09-03 接受日期:2025-10-20 出版日期:2026-04-08 发布日期:2026-04-08
通信作者:
孙权，E-mail：sunquan@mail.hzau.edu.cn。
胡大刚，E-mail：fap_296566@163.com。
联系方式: 苏一帆，E-mail：1218075188@qq.com。
基金资助:
国家自然科学基金(32572986); 国家自然科学基金(32302616); 泰山学者特聘专家项目(tstp20250723); 山东省重点研发计划（重大科技创新工程）(2023CXGC010709); 山东省自然科学基金(ZR2023QC032); 山东省青年科技人才托举工程(SDAST2024QTA025)

Effects of 2, 4-Epibrassinolide on Postharvest Storage Quality and Physiological Performance of Apple

SU YiFan¹(), YANG ZhanXu¹, WANG Di², MAO JunCheng¹, WEI MengMeng¹, CHEN Ze³, BAI XinRan³, CHU TianGe³, MA ChangNing¹, QIAO MingFei¹, SUN Quan¹^,⁴^,⁵^,^*(), HU DaGang¹^,^*()

¹ College of Horticultural Science and Engineering, Shandong Agricultural University/National Engineering Research Center for Apple/Key Laboratory of Biology and Germplasm Creation of Horticultural Crops in Huang-Huai Region, Ministry of Agriculture and Rural Affairs/Shandong Provincial Key Laboratory of Fruit and Vegetable Germplasm Innovation and Utilization, Taian 271018, Shandong
² Pingyi County Inspection and Testing Center, Pingyi 273300, Shandong
³ College of Future Technologies at Shandong Agricultural University, Taian 271018, Shandong
⁴ National Key Laboratory of Germplasm Innovation and Utilization for Horticultural Crops, Huazhong Agricultural University, Wuhan 430070
⁵ Hongshan Laboratory, Hubei, Wuhan 430070

Received:2025-09-03 Accepted:2025-10-20 Published:2026-04-08 Online:2026-04-08

摘要/Abstract

摘要：

【目的】本研究旨在系统探究2, 4-表油菜素内酯（2, 4-epibrassinolide，EBR）对苹果果实采后贮藏性能的调控作用，明确其在延缓果实软化、维持风味品质及增强抗氧化能力方面的生理与分子机制，为开发新型植物源保鲜剂提供理论依据和技术支持。【方法】以‘鲁丽’苹果为试验材料，采用外源施用3 μmol·L^-1 EBR溶液浸泡处理2 h，以蒸馏水处理为对照。果实处理后于室温条件下贮藏20 d，分别于第0、3、5和10天取样，测定果实硬度、脆性、果肉均质度等质地指标，乙烯释放速率，内源BR含量，BR信号通路关键基因MdBZR1及乙烯合成关键基因MdACS1、MdACO1的表达水平。同时，分析细胞壁组分（纤维素、原果胶、可溶性果胶）含量及其降解酶（α-Gal、β-Gal、PG、PME、β-GC等）活性变化，评估淀粉、可溶性糖（葡萄糖、果糖、蔗糖）、有机酸（苹果酸、柠檬酸）等风味物质的动态积累，并检测抗氧化酶（SOD、POD、CAT、APX）活性、活性氧（$\mathrm{O}_2^{\bar{.}}$、H₂O₂）水平以及抗逆物质（脯氨酸、可溶性蛋白、总酚、类黄酮）和丙二醛（MDA）含量。【结果】EBR处理显著延缓了苹果果实的软化进程，处理组果实的硬度、脆性和果肉均质度下降幅度分别比对照组降低2.88%、7.43%和4.63%。乙烯释放速率在贮藏中后期显著被抑制，第5天和第10天分别比对照组低37.11%和19.35%，同时乙烯合成关键基因MdACO1和MdACS1的表达显著下调。EBR处理促进内源BR积累，贮藏第10天时处理组BR含量较对照组高22.17%，并显著上调MdBZR1表达。细胞壁降解相关酶活性普遍被抑制，纤维素和原果胶降解分别减缓29.57%和16.32%，可溶性果胶积累降低53.00%。风味物质方面，EBR处理显著提高了可溶性糖（葡萄糖、果糖、蔗糖）和有机酸（苹果酸、柠檬酸）的含量，增强了果实的甜酸风味和营养品质。此外，EBR处理显著提升SOD、POD、CAT、APX等抗氧化酶活性，降低$\mathrm{O}_2^{\bar{.}}$和H₂O₂水平，减少MDA积累，同时提高脯氨酸、可溶性蛋白、总酚和类黄酮等抗逆物质的含量，综合增强了果实的抗氧化和抗衰老能力。【结论】EBR处理可通过激活BR信号通路、抑制乙烯合成、延缓细胞壁降解等细胞壁代谢活动、调控糖酸代谢平衡和增强抗氧化防御系统等多途径协同作用，显著改善苹果果实的采后贮藏性能和商品货架期。研究结果揭示了EBR在果实保鲜中的多重生理功能，也为基于植物激素的绿色保鲜技术开发提供了重要的理论支持和实践指导。

关键词: 苹果, 2, 4-表油菜素内酯, 采后, 贮藏性能, 保鲜, 细胞壁代谢

Abstract:

【Objective】This study systematically investigated the regulatory effects of 2, 4-epibrassinolide (EBR) on the postharvest storage quality of apple fruit. The physiological and molecular mechanisms of EBR delaying fruit softening, maintaining flavor quality, and enhancing antioxidant capacity were elucidated so as to offer a theoretical foundation and technical insight for developing novel plant-derived preservatives. 【Method】Using Luli apples as experimental material, fruits were treated by immersion in 3 μmol·L^-1 EBR solution for 2 hours, with a distilled water group serving as the control. After treatment, fruits were stored at room temperature for 20 days. Samples were collected at 0, 3, 5, and 10 days to assess textural properties (firmness, brittleness, and flesh homogeneity), ethylene production rate, endogenous BR levels, and the expression of key BR signaling gene MdBZR1, as well as ethylene biosynthesis genes MdACS1 and MdACO1. Changes in cell wall composition (cellulose, protopectin, and soluble pectin) and the activities of related degrading enzymes (α-Gal, β-Gal, PG, PME, and β-GC) were analyzed. The dynamics of flavor compounds were evaluated, including starch, soluble sugars (glucose, fructose, and sucrose) and organic acids (malic acid, and citric acid). Additionally, the activities of antioxidant enzymes (SOD, POD, CAT, and APX), levels of reactive oxygen species ($\mathrm{O}_2^{\bar{.}}$ and H₂O₂), content of stress-related metabolites (proline, soluble protein, total phenols, and flavonoids), and malondialdehyde (MDA) accumulation were measured.【Result】EBR treatment significantly delayed apple fruit softening. Reductions in firmness, brittleness, and flesh homogeneity in the EBR group were 2.88%, 7.43%, and 4.63% lower than those in the control, respectively. Ethylene production was significantly suppressed during mid-to-late storage, with rates 37.11% and 19.35% lower than the control on days 5 and 10, respectively. Expression of ethylene synthesis-related genes MdACO1 and MdACS1 was notably downregulated. EBR treatment promoted endogenous BR accumulation, resulting in 22.17% higher than the control on day 10, and significantly upregulated MdBZR1 expression. Activities of cell wall-degrading enzymes were generally suppressed, and degradation of cellulose and protopectin was delayed by 29.57% and 16.32%, respectively, while soluble pectin accumulation was reduced by 53.00%. In terms of flavor, EBR treatment significantly increased the content of soluble sugars (glucose, fructose, and sucrose) and organic acids (malic acid, and citric acid), enhancing the fruit’s sweet-sour taste and nutritional quality. Moreover, EBR significantly boosted the activities of SOD, POD, CAT, and APX, reduced $\mathrm{O}_2^{\bar{.}}$ and H₂O₂ levels, lowered MDA accumulation, and increased the contents of proline, soluble protein, total phenols, and flavonoids, collectively improving the fruit’s antioxidant capacity and delaying senescence.【Conclusion】EBR treatment significantly improves the postharvest storage performance and marketable shelf life of apple fruit. This improvement is achieved through the synergistic action of multiple coordinated pathways, which include activating the BR signaling pathway, suppressing ethylene biosynthesis, retarding cell wall degradation and associated metabolic activities, regulating the balance of sugar and acid metabolism, and enhancing the antioxidant defense system. This study not only revealed the multifaceted physiological roles of EBR in fruit preservation but also provided important theoretical and practical support for the development of hormone-based green preservation technologies.

Key words: apple, 2, 4-epibrassinolide, postharvest, storage performance, preservation, cell wall metabolism

苏一帆, 杨瞻旭, 王迪, 冒俊呈, 魏萌萌, 陈泽, 白欣冉, 楚天歌, 马昌宁, 乔明菲, 孙权, 胡大刚. 2, 4-表油菜素内酯对苹果果实采后贮藏性能的影响[J]. 中国农业科学, 2026, 59(7): 1536-1551.

SU YiFan, YANG ZhanXu, WANG Di, MAO JunCheng, WEI MengMeng, CHEN Ze, BAI XinRan, CHU TianGe, MA ChangNing, QIAO MingFei, SUN Quan, HU DaGang. Effects of 2, 4-Epibrassinolide on Postharvest Storage Quality and Physiological Performance of Apple[J]. Scientia Agricultura Sinica, 2026, 59(7): 1536-1551.

图/表 7

表1

图1

图2

图3

图4

图5

图6

参考文献 53

[1]	VALLÉE MARCOTTE B, VERHEYDE M, POMERLEAU S, DOYEN A, COUILLARD C. Health benefits of apple juice consumption: A review of interventional trials on humans. Nutrients, 2022, 14(4): 821. doi: 10.3390/nu14040821
[2]	强晓敏, 李秀红, 李进, 何芳婷, 王林海. 新形势下我国苹果产业高质量发展对策. 西北园艺(果树), 2021(4): 6-8.
	QIANG X M, LI X H, LI J, HE F T, WANG L H. China’s apple industry in the new situation under the high-quality development of countermeasures. Northwest Horticulture, 2021(4): 6-8. (in Chinese)
[3]	WANG J H, SUN Q, MA C N, WEI M M, WANG C K, ZHAO Y W, WANG W Y, HU D G. MdWRKY31-MdNAC 7 regulatory network: Orchestrating fruit softening by modulating cell wall-modifying enzyme MdXTH2 in response to ethylene signalling. Plant Biotechnology Journal, 2024, 22(12): 3244-3261. doi: 10.1111/pbi.v22.12
[4]	JIANG F, XU M Y, ZHANG H, LIU M, ZHAO L, DU G D. Ethylene promotes fruit softening of ‘Nanguo’ pear via cell wall degradation. Journal of Plant Growth Regulation, 2024, 43(12): 4770-4781. doi: 10.1007/s00344-024-11432-6
[5]	LAHAYE M, FALOURD X, LAILLET B, LE GALL S. Cellulose, pectin and water in cell walls determine apple flesh viscoelastic mechanical properties. Carbohydrate Polymers, 2020, 232: 115768. doi: 10.1016/j.carbpol.2019.115768
[6]	TSUCHIDA Y, YAKUSHIJI H, OE T, NEGORO K, GATO N, KOTANI T, ONISHI Y, KOBATA T, TAMURA M. Differences in cell-wall polysaccharide degradation during softening process in two cultivars of Japanese apricot fruits. Journal of the Japanese Society for Horticultural Science, 2014, 83(1): 81-89. doi: 10.2503/jjshs1.CH-067
[7]	FAN Z Q, BA L J, SHAN W, XIAO Y Y, LU W J, KUANG J F, CHEN J Y. A banana R2R3-MYB transcription factor MaMYB3 is involved in fruit ripening through modulation of starch degradation by repressing starch degradation-related genes and MabHLH6. The Plant Journal, 2018, 96(6): 1191-1205. doi: 10.1111/tpj.2018.96.issue-6
[8]	PEIRS A, SCHEERLINCK N, PEREZ A B, JANCSÓK P, NICOLAı̈ B M. Uncertainty analysis and modelling of the starch index during apple fruit maturation. Postharvest Biology and Technology, 2002, 26(2): 199-207. doi: 10.1016/S0925-5214(02)00038-8
[9]	SHIGA T M, SOARES C A, NASCIMENTO J R, PURGATTO E, LAJOLO F M, CORDENUNSI B R. Ripening-associated changes in the amounts of starch and non-starch polysaccharides and their contributions to fruit softening in three banana cultivars. Journal of the Science of Food and Agriculture, 2011, 91(8): 1511-1516. doi: 10.1002/jsfa.4342 pmid: 21445854
[10]	YAMAKI S. Isolation of vacuoles from immature apple fruit flesh and compartmentation of sugars, organic acids, phenolic compounds and amino acids. Plant and Cell Physiology, 1984, 25(1): 151-166.
[11]	COLARIC M, VEBERIC R, STAMPAR F, HUDINA M. Evaluation of peach and nectarine fruit quality and correlations between sensory and chemical attributes. Journal of the Science of Food and Agriculture, 2005, 85(15): 2611-2616. doi: 10.1002/jsfa.v85:15
[12]	YU J Q, GU K D, ZHANG L L, SUN C H, ZHANG Q Y, WANG J H, WANG C K, WANG W Y, DU M C, HU D G. MdbHLH 3 modulates apple soluble sugar content by activating phosphofructokinase gene expression. Journal of Integrative Plant Biology, 2022, 64(4): 884-900. doi: 10.1111/jipb.v64.4
[13]	JIANG B, FANG X J, FU D Q, WU W J, HAN Y C, CHEN H J, LIU R L, GAO H Y. Exogenous salicylic acid regulates organic acids metabolism in postharvest blueberry fruit. Frontiers in Plant Science, 2022, 13: 1024909. doi: 10.3389/fpls.2022.1024909
[14]	ZHENG X Z, GONG M, ZHANG Q D, TAN H Q, LI L P, TANG Y W, LI Z G, PENG M C, DENG W. Metabolism and regulation of ascorbic acid in fruits. Plants, 2022, 11(12): 1602. doi: 10.3390/plants11121602
[15]	SU J, LI M H, YANG H Q, SHU H L, YU K M, CAO H L, XU G Z, WANG M H, ZHU Y F, ZHU Y G, MA C H, SHAO J H. Enrichment of grape berries and tomato fruit with health-promoting tartaric acid by expression of the Vitis vinifera transketolase VvTK2 gene. International Journal of Biological Macromolecules, 2024, 257: 128734. doi: 10.1016/j.ijbiomac.2023.128734
[16]	WU J H, GAO H Y, ZHAO L, LIAO X J, CHEN F, WANG Z F, HU X S. Chemical compositional characterization of some apple cultivars. Food Chemistry, 2007, 103(1): 88-93. doi: 10.1016/j.foodchem.2006.07.030
[17]	ZHANG Y Z, LI P M, CHENG L L. Developmental changes of carbohydrates, organic acids, amino acids, and phenolic compounds in ‘Honeycrisp’ apple flesh. Food Chemistry, 2010, 123(4): 1013-1018. doi: 10.1016/j.foodchem.2010.05.053
[18]	ALI KHAN S, BEEKWILDER J, SCHAART J G, MUMM R, SORIANO J M, JACOBSEN E, SCHOUTEN H J. Differences in acidity of apples are probably mainly caused by a malic acid transporter gene on LG16. Tree Genetics & Genomes, 2013, 9(2): 475-487.
[19]	YANG C, CHEN T, SHEN B R, SUN S X, SONG H Y, CHEN D, XI W P. Citric acid treatment reduces decay and maintains the postharvest quality of peach (Prunus persica L.) fruit. Food Science & Nutrition, 2019, 7(11): 3635-3643. doi: 10.1002/fsn3.v7.11
[20]	HU Y F, HAO Y C, WEI Z Y, CUI H Y, ZHAN Y X. Effect of 1-MCP coupling with carbon dioxide treatment on antioxidant enzyme activities and quality of fresh-cut Fuji apples. Journal of Food Processing and Preservation, 2020, 44(12): e14903.
[21]	LV J Y, TAI R, CAO Y, GE Y H, CHEN J X, LI J R. Genome-wide identification and comparative expression analysis of ascorbate peroxidase (APX) gene family in apple fruit under 1-methylcyclopropene (1-MCP) and ethephon treatments during ripening. Scientia Horticulturae, 2024, 329: 113016. doi: 10.1016/j.scienta.2024.113016
[22]	FENG Z Q, LI T, LI X Y, LUO L X, LI Z, LIU C L, GE S F, ZHU Z L, LI Y Y, JIANG H, JIANG Y M. Enhancement of apple stress resistance via proline elevation by sugar substitutes. International Journal of Molecular Sciences, 2024, 25(17): 9548. doi: 10.3390/ijms25179548
[23]	HIDALGO M, SÁNCHEZ-MORENO C, DE PASCUAL-TERESA S. Flavonoid-flavonoid interaction and its effect on their antioxidant activity. Food Chemistry, 2010, 121(3): 691-696. doi: 10.1016/j.foodchem.2009.12.097
[24]	JIA D F, LIAO G L, YE B, ZHONG M, HUANG C H, XU X B. Changes in fruit quality, phenolic compounds, and antioxidant activity of kiwifruit (Actinidia eriantha) during on-vine ripening. LWT-Food Science and Technology, 2024, 206: 116564. doi: 10.1016/j.lwt.2024.116564
[25]	孙嘉茂, 崔全石, 王语晴, 司雅静, 时瑀繁, 卜海东, 袁晖, 王爱德. 苹果采前喷施EBR与MeJA对采后品质的影响. 园艺学报, 2022, 49(10): 2236-2248. doi: 10.16420/j.issn.0513-353x.2022-0594
	SUN J M, CUI Q S, WANG Y Q, SI Y J, SHI Y F, BU H D, YUAN H, WANG A D. Effects of brassinolides and methyl jasmonate spraying on the postharvest quality of apple fruit. Acta Horticulturae Sinica, 2022, 49(10): 2236-2248. (in Chinese) doi: 10.16420/j.issn.0513-353x.2022-0594
[26]	WARANG O, BHATTACHARJEE P, DEBBARMA S, CHANDER S. Brassinosteroids: Unveiling their role in fruit ripening and quality. Applied Fruit Science, 2025, 67(2): 82. doi: 10.1007/s10341-025-01304-y
[27]	MANGHWAR H, HUSSAIN A, ALI Q, LIU F. Brassinosteroids (BRs) role in plant development and coping with different stresses. International Journal of Molecular Sciences, 2022, 23(3): 1012. doi: 10.3390/ijms23031012
[28]	SUN Y, FAN X Y, CAO D M, TANG W Q, HE K, ZHU J Y, HE J X, BAI M Y, ZHU S W, OH E, et al. Integration of brassinosteroid signal transduction with the transcription network for plant growth regulation in Arabidopsis. Developmental Cell, 2010, 19(5): 765-777. doi: 10.1016/j.devcel.2010.10.010
[29]	YU X F, LI L, ZOLA J, ALURU M, YE H X, FOUDREE A, GUO H Q, ANDERSON S, ALURU S, LIU P, RODERMEL S, YIN Y H. A brassinosteroid transcriptional network revealed by genome-wide identification of BESI target genes in Arabidopsis thaliana. The Plant Journal, 2011, 65(4): 634-646. doi: 10.1111/tpj.2011.65.issue-4
[30]	NOLAN T M, VUKAŠINOVIĆ N, LIU D R, RUSSINOVA E, YIN Y H. Brassinosteroids: Multidimensional regulators of plant growth, development, and stress responses. The Plant Cell, 2020, 32(2): 295-318. doi: 10.1105/tpc.19.00335 pmid: 31776234
[31]	LV M H, LI J. Molecular mechanisms of brassinosteroid-mediated responses to changing environments in Arabidopsis. International Journal of Molecular Sciences, 2020, 21(8): 2737. doi: 10.3390/ijms21082737
[32]	SHI H Y, LI X P, LV M H, LI J. BES1/BZR1 family transcription factors regulate plant development via brassinosteroid-dependent and independent pathways. International Journal of Molecular Sciences, 2022, 23(17): 10149. doi: 10.3390/ijms231710149
[33]	CHAI Y M, ZHANG Q, TIAN L, LI C L, XING Y, QIN L, SHEN Y Y. Brassinosteroid is involved in strawberry fruit ripening. Plant Growth Regulation, 2013, 69(1): 63-69. doi: 10.1007/s10725-012-9747-6
[34]	LI J Z, GUO T T, GUO M L, DAI X N, XU X F, LI Y J, SONG Z Z, LIANG M X. Exogenous BR delayed peach fruit softening by inhibiting pectin degradation enzyme genes. Frontiers in Plant Science, 2023, 14: 1226921. doi: 10.3389/fpls.2023.1226921
[35]	PAN R J, GUO M L, GUO T T, WANG X Y, CHU Z J, LI J Z, YANG J J, LIANG M X. The shelf life of sweet cherry fruit was prolonged under brassinolide treatment. Journal of Food Measurement and Characterization, 2025, 19(8): 5720-5729. doi: 10.1007/s11694-025-03350-2
[36]	JI Y L, QU Y, JIANG Z Y, YAN J J, CHU J F, XU M Y, SU X, YUAN H, WANG A D. The mechanism for brassinosteroids suppressing climacteric fruit ripening. Plant Physiology, 2021, 185(4): 1875-1893. doi: 10.1093/plphys/kiab013 pmid: 33743010
[37]	MOU Y L, DONG X G, ZHANG Y, TIAN L M, HUO H L, QI D, XU J Y, LIU C, LI N M, YIN C, YANG X. Identification and evaluation of flesh texture of crisp pear fruit based on penetration test using texture analyzer. Horticulturae, 2025, 11(4): 359. doi: 10.3390/horticulturae11040359
[38]	孙超, 黎家. 油菜素甾醇类激素的生物合成、代谢及信号转导. 植物生理学报, 2017, 53(3): 291-307.
	SUN C, LI J. Biosynthesis, catabolism, and signal transduction of brassinosteroids. Plant Physiology Communications, 2017, 53(3): 291-307. (in Chinese)
[39]	JIA C G, ZHAO S K, BAO T T, ZHAO P Q, PENG K, GUO Q X, GAO X, QIN J C. Tomato BZR/BES transcription factor SlBZR1 positively regulates BR signaling and salt stress tolerance in tomato and Arabidopsis. Plant Science, 2021, 302: 110719. doi: 10.1016/j.plantsci.2020.110719
[40]	YANG X T, SONG J, CAMPBELL-PALMER L, FILLMORE S, ZHANG Z Q. Effect of ethylene and 1-MCP on expression of genes involved in ethylene biosynthesis and perception during ripening of apple fruit. Postharvest Biology and Technology, 2013, 78: 55-66. doi: 10.1016/j.postharvbio.2012.11.012
[41]	YANG S F, HOFFMAN N E. Ethylene biosynthesis and its regulation in higher plants. Annual Review of Plant Physiology, 1984, 35: 155-189. doi: 10.1146/arplant.1984.35.issue-1
[42]	LU S W, ZHANG Y, ZHU K J, YANG W, YE J L, CHAI L J, XU Q, DENG X X. The Citrus transcription factor CsMADS6 modulates carotenoid metabolism by directly regulating carotenogenic genes. Plant Physiology, 2018, 176(4): 2657-2676. doi: 10.1104/pp.17.01830
[43]	MUIR J G, ROSE R, ROSELLA O, LIELS K, BARRETT J S, SHEPHERD S J, GIBSON P R. Measurement of short-chain carbohydrates in common Australian vegetables and fruits by high-performance liquid chromatography (HPLC). Journal of Agricultural and Food Chemistry, 2009, 57(2): 554-565. doi: 10.1021/jf802700e pmid: 19123815
[44]	BUYSSE J, MERCKX R. An improved colorimetric method to quantify sugar content of plant tissue. Journal of Experimental Botany, 1993, 44(267): 1627-1629. doi: 10.1093/jxb/44.10.1627
[45]	HU L Y, HU S L, WU J, LI Y H, ZHENG J L, WEI Z J, LIU J, WANG H L, LIU Y S, ZHANG H. Hydrogen sulfide prolongs postharvest shelf life of strawberry and plays an antioxidative role in fruits. Journal of Agricultural and Food Chemistry, 2012, 60(35): 8684-8693. doi: 10.1021/jf300728h
[46]	SEPPÄ L, PELTONIEMI A, TAHVONEN R, TUORILA H. Flavour and texture changes in apple cultivars during storage. LWT-Food Science and Technology, 2013, 54(2): 500-512. doi: 10.1016/j.lwt.2013.06.012
[47]	VIDYA VARDHINI B, RAO S S R. Acceleration of ripening of tomato pericarp discs by brassinosteroids. Phytochemistry, 2002, 61(7): 843-847. doi: 10.1016/s0031-9422(02)00223-6 pmid: 12453577
[48]	ZAHARAH S S, SINGH Z, SYMONS G M, REID J B. Role of brassinosteroids, ethylene, abscisic acid, and indole-3-acetic acid in mango fruit ripening. Journal of Plant Growth Regulation, 2012, 31(3): 363-372. doi: 10.1007/s00344-011-9245-5
[49]	LI H, YE K Y, SHI Y T, CHENG J K, ZHANG X Y, YANG S H. BZR1 positively regulates freezing tolerance via CBF-dependent and CBF-independent pathways in Arabidopsis. Molecular Plant, 2017, 10(4): 545-559. doi: 10.1016/j.molp.2017.01.004
[50]	CAO S, ZHENG Y, YANG Z. Effect of 1-MCP treatment on nutritive and functional properties of loquat fruit during cold storage. New Zealand Journal of Crop and Horticultural Science, 2011, 39(1): 61-70. doi: 10.1080/01140671.2010.526621
[51]	VAN BUREN J P. The chemistry of texture in fruits and vegetables. Journal of Texture Studies, 1979, 10(1): 1-23. doi: 10.1111/jts.1979.10.issue-1
[52]	VORAGEN A G J, COENEN G J, VERHOEF R P, SCHOLS H A. Pectin, a versatile polysaccharide present in plant cell walls. Structural Chemistry, 2009, 20(2): 263-275. doi: 10.1007/s11224-009-9442-z
[53]	XIA X J, FANG P P, GUO X, QIAN X J, ZHOU J, SHI K, ZHOU Y H, YU J Q. Brassinosteroid-mediated apoplastic H₂O₂- glutaredoxin 12/14 cascade regulates antioxidant capacity in response to chilling in tomato. Plant, Cell & Environment, 2018, 41(5): 1052-1064. doi: 10.1111/pce.v41.5

基因 Gene	正向引物 Forward primer (5′-3′)	反向引物 Reverse primer (5′-3′)
MdACS1	GAGAAGACAGTCGCTCACG	GGCTACCTTTCATCTACCG
MdACO1	CGGATGGGACCAGAATG	CGTTGCCTGGGTTGTAG
MdBZR1	AACGGTAATGCTGATGCG	GACGTGGCAGAGGATGA
MdDWF4	TTATTCTCTGTCTTCTTCCATCAATTGTA	GCGGGAGATTGAGTCTGGTT
MdROT3	ATGGGATGGGTTTTGGGATG	TGGAACTTGACCCTTTTGATTTTC
18S	ACACGGGGAGGTAGTGACAA	CCTCCAATGGATCCTCGTTA

2, 4-表油菜素内酯对苹果果实采后贮藏性能的影响

Effects of 2, 4-Epibrassinolide on Postharvest Storage Quality and Physiological Performance of Apple

RichHTML

PDF

赞

可视化

摘要/Abstract

引用本文

使用本文

图/表 7

参考文献 53

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	从琪琪, 张静怡, 孟祥龙, 戴蓬博, 李波, 胡同乐, 王树桐, 曹克强, 王亚南. 我国苹果轮纹病弱毒菌株中病毒的鉴定及其携带情况的检测[J]. 中国农业科学, 2025, 58(3): 478-492.
[2]	覃璐, 谌丹丹, 江晓丽, 谢合平, 敖义俊, 杨阳, 朱峰, 许让伟, 廖文月, 程运江. 水分状态对连阴雨天气采收柑橘果实贮藏性能的影响[J]. 中国农业科学, 2025, 58(24): 5259-5273.
[3]	潘媛, 王德, 刘楠, 孟祥龙, 戴蓬博, 李波, 胡同乐, 王树桐, 曹克强, 王亚南. 两种高通量测序技术鉴定苹果病毒效果评价及两种新病毒的鉴定[J]. 中国农业科学, 2025, 58(2): 266-280.
[4]	秦佳昕, 欧虑索, 许晨郗, 邹运乾, 陆成确, 朱峰, 许让伟, 陈香玲, 秦克锋, 李果果, 程运江. 氯氟吡氧乙酸异辛酯对沃柑留树保鲜品质的影响[J]. 中国农业科学, 2025, 58(19): 3970-3984.
[5]	黄皓, 吴庆红, 张宇, 汪冶, 刘青娥, 方艺达, 罗自生. 新型相变蓄冷剂对香菇采后品质的影响[J]. 中国农业科学, 2024, 57(5): 989-999.
[6]	王文军, 江海燕, 田昊, 孟阔, 苟雯青. 双孢蘑菇采后病害及控制技术研究进展[J]. 中国农业科学, 2024, 57(23): 4794-4805.
[7]	张毅, 刘颖, 程存刚, 李燕青, 李壮. 牛粪与化肥配施比例对苹果园土壤有机碳库和酶活性的影响[J]. 中国农业科学, 2024, 57(20): 4107-4118.
[8]	周罕觅, 马林爽, 孙旗立, 陈佳庚, 李纪琛, 苏裕民, 陈诚, 吴奇. 基于多目标综合评价的苹果水氮综合调控[J]. 中国农业科学, 2024, 57(18): 3654-3670.
[9]	曾艳鑫, 宫昊楠, 由春香, 卢景生, 高文胜, 王小非. 不同砧木对多主干树形‘瑞香红’苹果幼树树体生长及果实品质的影响[J]. 中国农业科学, 2024, 57(14): 2847-2861.
[10]	张健, 赵彬森, 冯浩, 黄丽丽. Vm-milRN7调控苹果树腐烂病菌致病的功能及机理[J]. 中国农业科学, 2024, 57(10): 1930-1942.
[11]	张海青, 张恒涛, 高启明, 姚家龙, 王亚荣, 刘珍珍, 孟祥鹏, 周喆, 阎振立. 转录组分析筛选调控苹果分枝能力的关键基因[J]. 中国农业科学, 2024, 57(10): 1995-2009.
[12]	孙政, 赖忠晓, 赵晓敏, 江志利, 陈光友, 马志卿. 渭北旱塬苹果病虫害全程生物防控技术应用效果评价[J]. 中国农业科学, 2023, 56(6): 1102-1112.
[13]	郑文燕, 常源升, 何平, 何晓文, 王森, 高文胜, 李林光, 王海波. ‘鲁丽’×‘红1#’苹果杂交群体全基因组KASP标记开发及验证[J]. 中国农业科学, 2023, 56(5): 935-950.
[14]	王子盾, 王辉, 冯郁晨, 张学良, 闫雷玉, 刘小杰, 赵政阳. 不同颜色育果袋对‘瑞雪’苹果果实品质的影响[J]. 中国农业科学, 2023, 56(4): 729-740.
[15]	李星星, 周国富, 骆官雨, 陈思蓉, 张金龙, 陈国华, 张晓明. 橘小实蝇对不同品种苹果的选择偏好及适应性[J]. 中国农业科学, 2023, 56(17): 3358-3371.