大豆生育期基因E1和E2的启动子克隆及其表达模式分析

doi:10.3864/j.issn.0578-1752.2025.05.002

中国农业科学 ›› 2025, Vol. 58 ›› Issue (5): 840-850.doi: 10.3864/j.issn.0578-1752.2025.05.002

• 作物遗传育种·种质资源·分子遗传学 • 上一篇下一篇

大豆生育期基因E1和E2的启动子克隆及其表达模式分析

刘路平¹(), 胡雪洁¹, 祁金¹, 陈强¹, 刘智¹, 赵田湉¹, 史晓蕾¹, 刘兵强¹, 孟庆民¹, 张孟臣¹, 韩天富²(), 杨春燕¹()

¹ 河北省农林科学院粮油作物研究所/河北省作物遗传育种实验室/农业农村部黄淮海大豆生物学与遗传育种重点实验室，石家庄 050035
² 中国农业科学院作物科学研究所/农业农村部北京大豆生物学重点实验室，北京 100081

收稿日期:2024-07-02 接受日期:2024-08-05 出版日期:2025-03-07 发布日期:2025-03-07
通信作者:
韩天富，E-mail：hantianfu@caas.cn。
杨春燕，E-mail：chyyang66@163.com
联系方式: 刘路平，Tel：13121223199；E-mail：llp0322@yeah.net。
基金资助:
河北省农林科学院科技创新人才队伍建设项目(C22R0303); 国家大豆产业技术体系(CARS-04-PS09)

Cloning of the Promoters and Analysis of Expression Patterns of Maturity Genes E1 and E2 in Soybean

LIU LuPing¹(), HU XueJie¹, QI Jin¹, CHEN Qiang¹, LIU Zhi¹, ZHAO TianTian¹, SHI XiaoLei¹, LIU BingQiang¹, MENG QingMin¹, ZHANG MengChen¹, HAN TianFu²(), YANG ChunYan¹()

¹ Institute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences/Hebei Laboratory of Crop Genetis and Breeding/Huang-Huai-Hai Key Laboratory of Biology and Genetic Improvement of Soybean, Ministry of Agriculture and Rural Affairs, Shijiazhuang 050035
² Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/Key Laboratory of Soybean Biology (Beijing), Ministry of Agriculture and Rural Affairs, Beijing 100081

Received:2024-07-02 Accepted:2024-08-05 Published:2025-03-07 Online:2025-03-07

摘要/Abstract

摘要：

【目的】生育期是衡量大豆生态适应性的重要指标，也是关乎其产量形成的重要性状。研究大豆生育期主效基因E1和E2启动子及其表达模式，为生育期基因功能研究及其分子调控网络的解析提供依据，为大豆品种适应性改良及产量提高奠定基础。【方法】通过启动子顺式元件分析网站PlantCARE分析生育期基因E1和E2的启动子序列，检测其中包含的重要调控元件。克隆E1和E2的启动子，构建GUS表达载体，并进行拟南芥遗传转化，检测转基因植株不同组织器官的GUS活性。在弱光和强光条件下，比较长日照和短日照下E1和E2表达量的差异。在不同生育期组大豆品种中检测E1和E2表达量，分析其表达量与大豆品种生育期的相关性。【结果】E1和E2的启动子序列均包含AE-box、Box4和G-box等多个光反应元件，另外，E1启动子区还包含生长素、脱落酸等响应元件，E2启动子区包含低温、干旱等响应元件及分生组织表达相关元件。转基因拟南芥GUS活性检测发现，E1启动子在植株整个生长期各器官均有较强转录活性，E2启动子在幼苗下胚轴、叶片和根的维管组织中具有较强转录活性。在弱光和强光条件下，E1表达量均表现为长日照高于短日照。在弱光条件下，E2表达量表现为短日照高于长日照；在强光条件下，E2表达量表现为长日照高于短日照。随不同大豆品种生育期的延长，E1表达量呈现逐渐升高趋势，E2表达量无规律性变化。【结论】E1的启动子是一个广泛表达的启动子，该基因的表达量显著受光周期调控，与大豆品种生育期有明显相关性；E2的启动子在各器官维管组织有较强表达，在强光和弱光条件下，该基因受光周期调控模式存在差异，其表达量与大豆生育期无明显相关性。

关键词: 大豆, 生育期, 基因E1, 基因E2, 启动子, 表达模式

Abstract:

【Objective】Maturity time is an essential phenotypic measure of ecological adaptability of soybean and an important trait related to its yield formation. The study of promoters and expression patterns of major maturity genes E1 and E2 would provide basis for the study of gene function and molecular regulatory network of maturity time and lay foundation for adaptability improvement and yield increase in soybean.【Method】The promoter sequences of major maturity genes E1 and E2 were analyzed through the promoter cis-element analysis website PlantCARE, and the important regulatory elements were detected. The promoters of E1 and E2 were cloned, the GUS vectors were constructed, and transformation of Arabidopsis was performed to detect GUS activity in different tissues and organs of transgenic plants. Under low light and strong light conditions, the expression levels of E1 and E2 were compared between long day and short day conditions. The expression levels of E1 and E2 were detected in soybean varieties of different maturity groups, which is for the analysis of correlation between expression levels and maturity time of soybean varieties.【Result】Both E1 and E2 promoters contained multiple photoresponsive elements such as AE-box, Box4 and G-box, E1 promoter also contained auxin-response, abolic acid-response elements, and E2 promoter also contained low temperature-response, drought-response elements and meristem expression elements. In GUS activity detection of transgenic Arabidopsis, E1 promoter had strong transcriptional activity in all organs of the plant, and transcriptional activity of E2 promoter in fibrovascular tissues of seedling hypocotyl, leaf and root was relatively strong. Under both low light and strong light conditions, the expression level of E1 was significantly higher in long day than in short day. Under low light conditions, the expression level of E2 was higher in short day than in long day. Under strong light conditions, the expression level of E2 was higher in long day than in short day. With the increase of maturity time of different soybean varieties, expression level of E1 increased gradually, while E2 expression level did not change regularly.【Conclusion】The promoter of E1 gene was a widely expressed promoter, and its expression level was significantly regulated by photoperiod and significantly correlated with the maturity time of soybean varieties. The promoter of E2 was strongly expressed in vascular tissues of various organs, the photoperiodic regulation mode of this gene was different under strong light and low light conditions, and there was no significant correlation between expression level of E2 and maturity time.

Key words: soybean, maturity time, maturity gene E1, maturity gene E2, promoter, expression pattern

刘路平, 胡雪洁, 祁金, 陈强, 刘智, 赵田湉, 史晓蕾, 刘兵强, 孟庆民, 张孟臣, 韩天富, 杨春燕. 大豆生育期基因E1和E2的启动子克隆及其表达模式分析[J]. 中国农业科学, 2025, 58(5): 840-850.

LIU LuPing, HU XueJie, QI Jin, CHEN Qiang, LIU Zhi, ZHAO TianTian, SHI XiaoLei, LIU BingQiang, MENG QingMin, ZHANG MengChen, HAN TianFu, YANG ChunYan. Cloning of the Promoters and Analysis of Expression Patterns of Maturity Genes E1 and E2 in Soybean[J]. Scientia Agricultura Sinica, 2025, 58(5): 840-850.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2025.05.002

https://www.chinaagrisci.com/CN/Y2025/V58/I5/840

图/表 7

表1

图1

图2

图3

图4

图5

图6

参考文献 42

[1]	ZHAO L, LI M M, XU C J, YANG X, LI D M, ZHAO X, WANG K, LI Y H, ZHANG X M, LIU L X, DING F Q, DU H L, WANG C S, SUN J Z, LI W B. Natural variation in GmGBP1 promoter affects photoperiod control of flowering time and maturity in soybean. The Plant Journal, 2018, 96(1): 147-162.
[2]	LI X M, FANG C, YANG Y Q, LV T X, SU T, CHEN L Y, NAN H Y, LI S C, ZHAO X H, LU S J, DONG L D, CHENG Q, TANG Y, XU M L, ABE J, HOU X L, WELLER J L, KONG F J, LIU B H. Overcoming the genetic compensation response of soybean florigens to improve adaptation and yield at low latitudes. Current Biology, 2021, 31(17): 3755-3767.
[3]	KANTOLIC A G, SLAFER G A. Development and seed number in indeterminate soybean as affected by timing and duration of exposure to long photoperiods after flowering. Annals of Botany, 2007, 99(5): 925-933. doi: 10.1093/aob/mcm033 pmid: 17452381
[4]	韩天富, 盖钧镒, 邱家驯. 中国大豆不同生态类型代表品种开花前, 开花后光周期反应的比较研究. 大豆科学, 1998, 17(2): 129-134.
	HAN T F, GAI J Y, QIU J X. A comparative study on pre- and post- flowering photoperiod response in various ecotypes of soybeans. Soybean Science, 1998, 17(2): 129-134. (in Chinese)
[5]	SONG W W, SUN S, IBRAHIM S E, XU Z J, WU H Y, HU X G, JIA H C, CHENG Y X, YANG Z L, JIANG S B, WU T T, SINEGOVSKII M, SAPEY E, NEPOMUCENO A, JIANG B J, HOU W S, SINEGOVSKAYA V, WU C X, GAI J Y, HAN T F. Standard cultivar selection and digital quantification for precise classification of maturity groups in soybean. Crop Science, 2019, 59(5): 1997-2006.
[6]	SONG W W, LIU L P, SUN S, WU T T, ZENG H Y, TIAN S Y, SUN B C, LI W B, LIU L J, WANG S M, XING H, ZHOU X A, NIAN H, LU W C, HAN X Z, WANG S Y, CHEN W Y, GUO T, SONG X Q, TIAN Z Y, CHENG Y X, SONG S H, FU L S, WANG H C, LUO R P, LIU X Y, LIU Q, ZHANG G H, LU S H, XU R, LI S Z, LU W G, ZHANG Q, WANG Z B, JIANG C G, SHEN W L, ZHANG M R, ZHU D H, WANG R Z, CHEN Y, WANG T J, ZHU X T, ZHAN Y, JIANG B J, XU C L, YUAN S, HOU W S, GAI J Y, WU C X, HAN T F. Precise classification and regional delineation of maturity groups in soybean cultivars across China. European Journal of Agronomy, 2023, 151(3): 126982.
[7]	XIA Z J, WATANABE S, YAMADA T, TSUBOKURA Y, NAKASHIMA H, ZHAI H, ANAI T, SATO S, YAMAZAKI T, LÜ S X, WU H Y, TABATA S, HARADA K. Positional cloning and characterization reveal the molecular basis for soybean maturity locus E1 that regulates photoperiodic flowering. Proceedings of the National Academy of Sciences of the United States of America, 2012, 109(32): E2155- E2164.
[8]	WATANABE S, XIA Z J, HIDESHIMA R, TSUBOKURA Y, SATO S, YAMANAKA N, TAKAHASHI R, ANAI T, TABATA S, KITAMURA K, HARADA K. A map-based cloning strategy employing a residual heterozygous line reveals that the GIGANTEA gene is involved in soybean maturity and flowering. Genetics, 2011, 188(2): 395-407.
[9]	WATANABE S, HIDESHIMA R, XIA Z J, TSUBOKURA Y, SATO S, NAKAMOTO Y, YAMANAKA N, TAKAHASHI R, ISHIMOTO M, ANAI T, TABATA S, HARADA K. Map-based cloning of the gene associated with the soybean maturity locus E3. Genetics, 2009, 182(4): 1251-1262.
[10]	LIU B H, KANAZAWA A, MATSUMURA H, TAKAHASHI R, HARADA K, ABE J. Genetic redundancy in soybean photoresponses associated with duplication of the phytochrome A gene. Genetics. 2008, 180(2): 995-1007. doi: 10.1534/genetics.108.092742 pmid: 18780733
[11]	BONATO E R, VELLO N A. E6, a dominant gene conditioning early flowering and maturity in soybeans. Genetics and Molecular Biology, 1999, 22(2): 229-232.
[12]	COBER E R, VOLDENG H D. A new soybean maturity and photoperiod-sensitivity locus linked to E1 and T. Crop Science, 2001, 41(3): 698-701.
[13]	COBER E R, MOLNAR S J, CHARETTE M, VOLDENG H D. A new locus for early maturity in soybean. Crop Science, 2010, 50(2): 524-527.
[14]	KONG F J, NAN H Y, CAO D, LI Y, WU F F, WANG J L, LU S J, YUAN X H, COBER E R, ABE J, LIU B H. A new dominant gene E9 conditions early flowering and maturity in soybean. Crop Science, 2014, 54(6): 2529-2535.
[15]	SAMANFAR B, MOLNAR S J, CHARETTE M, SCHOENROCK A, DEHNE F, GOLSHANI A, BELZILE F, COBER E R. Mapping and identification of a potential candidate gene for a novel maturity locus, E10, in soybean. Theoretical and Applied Genetics, 2017, 130(2): 377-390.
[16]	WANG F F, NAN H Y, CHEN L Y, FANG C, ZHANG H Y, SU T, LI S C, CHENG Q, DONG L D, LIU B H, KONG F J, LU S J. A new dominant locus, E11, controls early flowering time and maturity in soybean. Molecular Breeding, 2019, 39(5): 70.
[17]	LU S J, DONG L D, FANG C, LIU S L, KONG L P, CHENG Q, CHEN L Y, SU T, NAN H Y, ZHANG D, ZHANG L, WANG Z J, YANG Y Q, YU D Y, LIU X L, YANG Q Y, LIN X Y, TANG Y, ZHAO X H, YANG X Q, TIAN C G, XIE Q G, LI X, YUAN X H, TIAN Z X, LIU B H, WELLER J L, KONG F J. Stepwise selection on homeologous PRR genes controlling flowering and maturity during soybean domestication. Nature Genetics, 2020, 52(4): 428-436.
[18]	DONG L D, LI S C, WANG L S, SU T, ZHANG C B, BI Y D, LAI Y C, KONG L P, WANG F, PEI X X, LI H Y, HOU Z H, DU H P, DU H, LI T, CHENG Q, FANG C, KONG F J, LIU B H. The genetic basis of high-latitude adaptation in wild soybean. Current Biology, 2023, 33(2): 252-262.
[19]	DONG L D, CHENG Q, FANG C, KONG L P, YANG H, HOU Z H, LI Y L, NAN H Y, ZHANG Y H, CHEN Q S, ZHANG C B, KOU K, SU T, WANG L S, LI S C, LI H Y, LIN X Y, TANG Y, ZHAO X H, LU S J, LIU B H, KONG F J. Parallel selection of distinct Tof5 alleles drove the adaptation of cultivated and wild soybean to high latitudes. Molecular Plant, 2022, 15(2): 308-321.
[20]	LI H Y, DU H P, HE M L, WANG J H, WANG F, YUAN W J, HUANG Z R, CHENG Q, GOU C J, CHEN Z, LIU B H, KONG F J, FANG C, ZHAO X H, YU D Y. Natural variation of FKF1 controls flowering and adaptation during soybean domestication and improvement. The New Phytologist, 2023, 238(4): 1671-1684.
[21]	LI H Y, DU H P, HUANG Z R, HE M L, KONG L P, FANG C, CHEN L Y, YANG H, ZHANG Y H, LIU B H, KONG F J, ZHAO X H. The AP2/ERF transcription factor TOE4b regulates photoperiodic flowering and grain yield per plant in soybean. Plant Biotechnology Journal, 2023, 21(8): 1682-1694.
[22]	DONG L D, FANG C, CHENG Q, SU T, KOU K, KONG L P, ZHANG C B, LI H Y, HOU Z H, ZHANG Y H, CHEN L Y, YUE L, WANG L S, WANG K, LI Y L, GAN Z R, YUAN X H, WELLER J L, LU S J, KONG F J, LIU B H. Genetic basis and adaptation trajectory of soybean from its temperate origin to tropics. Nature Communications, 2021, 12(1): 5445. doi: 10.1038/s41467-021-25800-3 pmid: 34521854
[23]	KOU K, YANG H, LI H Y, FANG C, CHEN L Y, YUE L, NAN H Y, KONG L P, LI X M, WANG F, WANG J H, DU H P, YANG Z Y, BI Y D, LAI Y C, DONG L D, CHENG Q, SU T, WANG L S, LI S C, HOU Z H, LU S J, ZHANG Y H, CHE Z J, YU D Y, ZHAO X H, LIU B H, KONG F J. A functionally divergent SOC1 homolog improves soybean yield and latitudinal adaptation. Current Biology, 2022, 32(8): 1728-1742.
[24]	LU S J, ZHAO X H, HU Y L, LIU S L, NAN H Y, LI X M, FANG C, CAO D, SHI X Y, KONG L P, SU T, ZHANG F G, LI S C, WANG Z, YUAN X H, COBER E R, WELLER J L, LIU B H, HOU X L, TIAN Z X, KONG F J. Natural variation at the soybean J locus improves adaptation to the tropics and enhances yield. Nature Genetics, 2017, 49(5): 773-779.
[25]	TSUBOKURA Y, WATANABE S, XIA Z J, KANAMORI H, YAMAGATA H, KAGA A, KATAYOSE Y, ABE J, ISHIMOTO M, HARADA K. Natural variation in the genes responsible for maturity loci E1, E2, E3 and E4 in soybean. Annals of Botany, 2014, 113(3): 429-441.
[26]	LIU L P, SONG W W, WANG L W, SUN X G, QI Y P, WU T T, SUN S, JIANG B J, WU C X, HOU W S, NI Z F, HAN T F. Allele combinations of maturity genes E1-E4 affect adaptation of soybean to diverse geographic regions and farming systems in China. PLoS ONE, 2020, 15(7): e0235397.
[27]	ZHAI H, LÜ S X, WU H Y, ZHANG Y P, ZHANG X Z, YANG J Y, WANG Y Y, YANG G, QIU H M, CUI T T, XIA Z J. Diurnal expression pattern, allelic variation, and association analysis reveal functional features of the E1 gene in control of photoperiodic flowering in soybean. PLoS ONE, 2015, 10(8): e0135909.
[28]	DISSANAYAKA A, RODRIGUEZ T O, DI S K, YAN F, GITHIRI S M, RODAS F R, ABE J, TAKAHASHI R. Quantitative trait locus mapping of soybean maturity gene E5. Breeding Science, 2016, 66(3): 407-415.
[29]	刘路平. 大豆生育期基因单倍型鉴定及地理分布规律研究[D]. 北京: 中国农业大学, 2020.
	LIU L P. Identification of soybean maturity gene haplotypes and study of their geographic distribution[D]. Beijing: China Agricultural University, 2020. (in Chinese)
[30]	CHANG S S, PARK S K, KIM B C, KANG B J, KIM D U, NAM H G. Stable genetic transformation of Arabidopsis thaliana by Agrobacterium inoculation in planta. The Plant Journal, 1994, 5(4): 551-558.
[31]	LIN X Y, LIU B H, WELLER J L, ABE J, KONG F J. Molecular mechanisms for the photoperiodic regulation of flowering in soybean. Journal of Integrative Plant Biology, 2021, 63(6):981-994. doi: 10.1111/jipb.13021
[32]	胡雪洁, 刘路平, 王凤敏, 韩玉华, 孙宾成, 马启彬, 黄志平, 冯燕, 陈强, 杨春燕, 张孟臣, 张锴, 秦君. 利用大豆生育期基因E1和E2构建适宜不同生态区的ms1基础轮回群体. 中国农业科学, 2024, 57(17): 3305-3317. doi:10.3864/j.issn.0578-1752.2024.17.001.
	HU X J, LIU L P, WANG F M, HAN Y H, SUN B C, MA Q B, HUANG Z P, FENG Y, CHEN Q, YANG C Y, ZHANG M C, ZHANG K, QIN J. Construction of ms1 basic recurrent populations adapted to different ecological regions using maturity genes E1 and E2 in soybean. Scientia Agricultura Sinica, 2024, 57(17): 3305-3317. doi:10.3864/j.issn.0578-1752.2024.17.001. (in Chinese)
[33]	LI Y L, HOU Z H, LI W W, LI H Y, LU S J, GAN Z R, DU H, LI T, ZHANG Y H, KONG F J, CHENG Y H, HE M L, MA L X, LIAO C M, LI Y R, DONG L D, LIU B H, CHENG Q. The legume-specific transcription factor E1 controls leaf morphology in soybean. BMC Plant Biology, 2021, 21(1):531. doi: 10.1186/s12870-021-03301-1 pmid: 34773981
[34]	WAN Z, LIU Y X, GUO D D, FAN R, LIU Y, XU K, ZHU J L, QUAN L, LU W T, BAI X, ZHAI H. CRISPR/Cas9-mediated targeted mutation of the E1 decreases photoperiod sensitivity, alters stem growth habits, and decreases branch number in soybean. Frontiers in Plant Science, 2022, 13: 1066820.
[35]	MISHRA P, PANIGRAHI K C. GIGANTEA-an emerging story. Frontiers in Plant Science, 2015, 6: 8.
[36]	MARTIN-TRYON E L, KREPS J A, HARMER S L. GIGANTEA acts in blue light signaling and has biochemically separable roles in circadian clock and flowering time regulation. Plant Physiology, 2007, 143(1): 473-486.
[37]	ZHAO X H, LI H Y, WANG L S, WANG J H, HUANG Z R, DU H P, LI Y R, YANG J H, HE M L, CHENG Q, LIN X Y, LIU B H, KONG F J. A critical suppression feedback loop determines soybean photoperiod sensitivity. Developmental Cell, 2024, 59(13): 1750-1763.
[38]	LI F, ZHANG X M, HU R B, WU F Q, MA J H, MENG Y, FU Y F. Identification and molecular characterization of FKF1 and GI homologous genes in soybean. PLoS ONE, 2013, 8(11): e79036.
[39]	吕世祥. 调控大豆E1基因表达的分子机理研究[D]. 长春: 中国科学院东北地理与农业生态研究所, 2015.
	LÜ S X. Studies on molecular mechanism regulating the expression of E1 in soybean [Glycine Max (L.) Merr.][D]. Changchun: Northeast Institute of Geography and Agroecology, Chinese Academy of Science, 2015. (in Chinese)
[40]	LIU W, JIANG B J, MA L M, ZHANG S W, ZHAI H, XU X, HOU W S, XIA Z J, WU C X, SUN S, WU T T, CHEN L, HAN T F. Functional diversification of Flowering Locus T homologs in soybean: GmFT1a and GmFT2a/5a have opposite roles in controlling flowering and maturation. The New Phytologist, 2018, 217(3): 1335-1345.
[41]	WANG Y, GU Y Z, GAO H H, QIU L J, CHANG R Z, CHEN S Y, HE C Y. Molecular and geographic evolutionary support for the essential role of GIGANTEAa in soybean domestication of flowering time. BMC Evolutionary Biology, 2016, 16: 79-91. doi: 10.1186/s12862-016-0653-9 pmid: 27072125
[42]	CAO D, TAKESHIMA R, ZHAO C, LIU B H, JUN A, KONG F J. Molecular mechanisms of flowering under long days and stem growth habit in soybean. Journal of Experimental Botany, 2017, 68(8): 1873-1884. doi: 10.1093/jxb/erw394 pmid: 28338712

引物名称Primer name	引物序列Primer sequence (5′-3′)	用途Purpose
proE1-F	CTTCAGACTCAGATGGTAGG	E1启动子克隆 Cloning of E1 promoter
proE1-R	GTTGGAAGAGATGAATAGGGTC	E1启动子克隆 Cloning of E1 promoter
proE2-F	CTTAGTACCACACCCTGGAC	E2启动子克隆 Cloning of E2 promoter
proE2-R	CTATTTACTTTCAAGGTATTG	E2启动子克隆 Cloning of E2 promoter
E1PT-F	CTTCAGACTCAGATGGTAGG	E1启动子转基因拟南芥检测 Detection of E1 promoter transgenic Arabidopsis
E1PT-R	AGTGTGACTCTACGTTCTCC
E2PT-F	AAGTGCAAACTTTATGGAGTA	E2启动子转基因拟南芥检测 Detection of E2 promoter transgenic Arabidopsis
E2PT-R	CCTCCTATGCAGCTTCAATC
qE1-F	CCACCATATGCGAAGCCTCTA	E1表达量检测 Detection of E1 expression level
qE1-R	GGTGCATGGATTTGGTGTCC	E1表达量检测 Detection of E1 expression level
qE2-F	CCAAAAATTGAGAATGAATACTCGG	E2表达量检测 Detection of E2 expression level
qE2-R	TAGTCACTTCGAATACCTACTGGTG	E2表达量检测 Detection of E2 expression level
qGmActin-F	CGGTGGTTCTATCTTGGCATC	大豆荧光定量内参基因 Reference gene of qRT-PCR in soybean
qGmActin-R	GTCTTTCGCTTCAATAACCCTA	大豆荧光定量内参基因 Reference gene of qRT-PCR in soybean
qGUS-F	CGAAGCGAGCAATGTGATGG	GUS表达量检测 Detection of GUS expression level
qGUS-R	GATCCGCAAGACGCATCAAC	GUS表达量检测 Detection of GUS expression level

大豆生育期基因E1和E2的启动子克隆及其表达模式分析

Cloning of the Promoters and Analysis of Expression Patterns of Maturity Genes E1 and E2 in Soybean

RichHTML

PDF

赞

可视化

摘要/Abstract

引用本文

使用本文

图/表 7

参考文献 42

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	肖长春, 韦新宇, 曾跃辉, 黄建鸿, 许旭明. 不同播期下黑米籽粒花色苷的积累特性及其与气象因子的关系[J]. 中国农业科学, 2025, 58(5): 890-906.
[2]	郑煜, 陈颐, 遆晋松, 史龙飞, 许校博, 李昱霖, 郭瑞. 烟草不同轮作模式碳足迹及经济效益评价[J]. 中国农业科学, 2025, 58(4): 733-747.
[3]	郭天发, 吴金龙, 仇倩倩, 马新超, 王力荣, 吴翠云. 新疆土桃果皮纯色形成与PpMYB10.1启动子变异的关系[J]. 中国农业科学, 2025, 58(2): 326-338.
[4]	王伟, 吴传磊, 胡晓渝, 李佳佳, 白鹏宇, 王郭伋, 苗龙, 王晓波. 大豆LOX基因家族的全基因组鉴定及GmLOX15A1基因等位变异对百粒重的影响[J]. 中国农业科学, 2025, 58(1): 10-29.
[5]	李荣德, 何平, 罗莉霞, 史梦雅, 侯乾, 马振国, 郭瑞星, 成洪涛. “稻-稻-油”生产模式下短生育期冬油菜品种选育与推广现状分析[J]. 中国农业科学, 2024, 57(5): 846-854.
[6]	熊楚雯, 郭智滨, 周强华, 程艳波, 马启彬, 蔡占东, 年海. 大豆转录因子NAC1耐低磷胁迫的功能研究[J]. 中国农业科学, 2024, 57(3): 442-453.
[7]	冯雯蜜, 周芳雪, 于哲, 牟可欣, 井妍, 李海燕. 大豆抗花叶病毒病基因GmRHF1的克隆及功能分析[J]. 中国农业科学, 2024, 57(23): 4632-4643.
[8]	李允静, 任雪贞, 肖芳, 晋芳, 高鸿飞, 景琦, 武玉花, 孙全, 李俊, 王培, 翟杉杉, 金石桥, 吴刚. 基于二重实时荧光PCR方法精准快速鉴定大豆转基因种子转化体纯度[J]. 中国农业科学, 2024, 57(23): 4698-4711.
[9]	王晶, 王天舒, 王丽, 周新雨, 李庭宇, 孟熠黎, 黄新阳, 尧水红. 麦茬土壤残留氮对夏大豆根瘤、根系发育及产量的影响[J]. 中国农业科学, 2024, 57(23): 4712-4724.
[10]	吴传磊, 胡晓渝, 王伟, 苗龙, 白鹏宇, 王郭伋, 李娜, 舒阔, 邱丽娟, 王晓波. 大豆油分相关功能基因分子标记开发与鉴定及优异等位变异聚合分析[J]. 中国农业科学, 2024, 57(22): 4402-4415.
[11]	董奎军, 张亦涛, 刘瀚文, 张继宗, 王伟军, 温延臣, 雷秋良, 文宏达. 玉米大豆间作减量施氮对当季作物农艺性状、经济效益和后茬小麦产量的影响[J]. 中国农业科学, 2024, 57(22): 4495-4506.
[12]	邵嘉朱, 吕雯, 廖鑫琳, 袁歆瑜, 宋振, 蒋冬花. 大豆根际促生菌的分离、鉴定及其耐盐促生作用[J]. 中国农业科学, 2024, 57(21): 4248-4263.
[13]	夏杨, 韩光杰, 李传明, 刘琴, 张楠, 黄立鑫, 陆玉荣, 徐彬, 徐健. 大豆-玉米带状复合种植模式下草地贪夜蛾的生存适应性和危害性[J]. 中国农业科学, 2024, 57(20): 4035-4044.
[14]	胡雪洁, 刘路平, 王凤敏, 韩玉华, 孙宾成, 马启彬, 黄志平, 冯燕, 陈强, 杨春燕, 张孟臣, 张锴, 秦君. 利用大豆生育期基因E1和E2构建适宜不同生态区的ms1基础轮回群体[J]. 中国农业科学, 2024, 57(17): 3305-3317.
[15]	杨立达, 彭新月, 朱文雪, 赵静, 袁晓婷, 林萍, 罗凯, 李易玲, 罗春明, 李雨泽, 杨文钰, 雍太文. 秸秆还田与灌溉方式对大豆玉米带状间作出苗及幼苗生长的影响[J]. 中国农业科学, 2024, 57(17): 3366-3383.