中国农业科学 ›› 2019, Vol. 52 ›› Issue (20): 3611-3624.doi: 10.3864/j.issn.0578-1752.2019.20.012
奚雅静1,2,汪俊玉1,2,李银坤3,武雪萍2(),李晓秀1(
),王碧胜2,李生平2,宋霄君2,刘彩彩2
收稿日期:
2019-06-03
接受日期:
2019-09-02
出版日期:
2019-10-16
发布日期:
2019-10-28
通讯作者:
武雪萍,李晓秀
作者简介:
奚雅静,E-mail:1051794571@qq.com。
基金资助:
YaJing XI1,2,JunYu WANG1,2,YinKun LI3,XuePing WU2(),XiaoXiu LI1(
),BiSheng WANG2,ShengPing LI2,XiaoJun SONG2,CaiCai LIU2
Received:
2019-06-03
Accepted:
2019-09-02
Online:
2019-10-16
Published:
2019-10-28
Contact:
XuePing WU,XiaoXiu LI
摘要:
【目的】 通过在有机肥基础上增施不同量无机氮,研究滴灌水肥一体化条件下温室番茄土壤N2O排放和脲酶(UR)、硝酸还原酶(NR)、亚硝酸还原酶(Ni R)以及羟胺还原酶(Hy R)活性的动态变化,分析各处理土壤N2O排放特征及土壤UR、NR、Ni R和Hy R活性对土壤N2O排放的影响,揭示在滴灌水肥一体化下N2O排放过程机制。【方法】 试验共设CK(不施氮)、N1(200 kg·hm -2有机氮)、N2(200 kg·hm -2有机氮+ 250 kg·hm -2无机氮)、N3(200 kg·hm -2有机氮+ 475 kg·hm -2无机氮)4个处理。采用静态箱-气相色谱法,对番茄生育期内土壤N2O排放、土壤酶活性、土壤温湿度等进行监测。【结果】 滴灌水肥一体化,各施氮处理均在施肥+灌溉后第1天出现N2O排放高峰,随着时间推移不断下降,不同处理番茄整个生育期N2O排放通量在0.98—1 544.79 μg·m -2·h -1。土壤N2O排放总量差异显著,依次为N3((7.13±0.11)kg·hm -2)>N2((4.87±0.21)kg·hm -2)>N1((2.54±0.17)kg·hm -2)>CK((1.56±0.23)kg·hm -2),与N3相比,处理N1、N2土壤N2O排放总量分别降低了64.38%、31.70%。番茄生育期内N2O季节排放特征明显,秋季高,冬季低。土壤氮素转化相关酶活性大致随施氮量的升高而增高。土壤N2O排放通量与5 cm土壤温度、0—10 cm土层硝态氮含量、土壤NR活性及土壤Hy R活性均呈极显著正相关(P<0.01)。【结论】 滴灌水肥一体化下,土壤微生物处于好气环境,土壤N2O主要来自于硝化过程,减少了由反硝化过程所产生的N2O排放。综合考虑番茄产量、品质、N2O排放等因素,推荐北方温室秋冬茬番茄施用200 kg·hm -2有机氮+250 kg·hm -2无机氮,75 kg·hm -2 P2O5,450 kg·hm -2 K2O较为适宜。
奚雅静,汪俊玉,李银坤,武雪萍,李晓秀,王碧胜,李生平,宋霄君,刘彩彩. 滴灌水肥一体化配施有机肥对土壤N2O排放与酶活性的影响[J]. 中国农业科学, 2019, 52(20): 3611-3624.
YaJing XI,JunYu WANG,YinKun LI,XuePing WU,XiaoXiu LI,BiSheng WANG,ShengPing LI,XiaoJun SONG,CaiCai LIU. Effects of Drip Irrigation Water and Fertilizer Integration Combined with Organic Fertilizers on Soil N2O Emission and Enzyme Activity[J]. Scientia Agricultura Sinica, 2019, 52(20): 3611-3624.
表3
土壤N2O排放通量与环境因子及无机氮含量的相关分析"
5 cm土温 5 cm soil temperature | 土壤含水量 Soil water content | 0-10 cm土层硝态氮含量 Nitrate nitrogen content in 0-10 cm soil layer | 0-10 cm土层铵态氮含量 Ammonium nitrogen content in 0-10 cm soil layer | |
---|---|---|---|---|
CK | 0.278 | 0.134 | 0.124 | 0.102 |
N1 | 0.319** | 0.158 | 0.506** | 0.155 |
N2 | 0.401** | 0.237 | 0.766** | 0.351 |
N3 | 0.445** | 0.352 | 0.820** | 0.512** |
表4
土壤氮素转化酶活性与N2O排放通量的相关分析"
N2O排放通量 N2O emission flux | UR活性 UR activity | NR活性 NR activity | Ni R活性 Ni R activity | Hy R活性 Hy R activity | |
---|---|---|---|---|---|
N2O排放通量 N2O emission flux | 1 | -0.055 | 0.516** | 0.163 | 0.757** |
UR活性 UR activity | 1 | 0.704** | 0.843** | 0.567** | |
NR活性 NR activity | 1 | 0.605** | 0.712** | ||
Ni R活性 Ni R activity | 1 | 0.566** | |||
Hy R活性 Hy R activity | 1 |
[1] | HUTCHINSON G L, MOSIER A R . Improved soil cover method for field measurement of nitrous oxide fluxes. Soil Science Society of America Journal, 1981,45(2):311. |
[2] | Delgado J A, Mosier A R . Mitigation alternatives to decrease nitrous oxides emissions and urea-nitrogen loss and their effect on methane flux[J]. Journal of Environmental Quality, 1996,25(5):1105-1111. |
[3] | 张秀君, 徐慧, 陈冠雄 . 长白山阔叶红松林树木N2O排放及总量初步估算. 生态学杂志, 2004,23(5):232-235. |
ZHANG X J, XU H, CHEN G X . N2O emission and total estimation of trees in broad-leaved Korean Pine Forest in Changbai Mountain. Chinese Journal of Ecology, 2004,23(5):232-235.(in Chinese) | |
[4] | BOUWMAN A F . The role of soils and land use in the greenhouse effect. Netherlands Journal of Agricultural Science, 1989,37(1):13-19. |
[5] | 李银坤, 武雪萍, 郭文忠, 薛绪掌 . 不同氮水平下黄瓜-番茄日光温室栽培土壤N2O排放特征. 农业工程学报, 2014,30(23):260-267. |
LI Y K, WU X P, GUO W Z, XUE X Z . Characteristics of N2O emission from cucumber-tomato in greenhouse cultivation under different nitrogen levels. Transaction of the Chinese Agricultural Engineering, 2014,30(23):260-267. (in Chinese) | |
[6] |
闫鹏, 武雪萍, 华珞, 武其甫, 李银坤, 吴会军, 王小彬, 蔡典雄, 张彦才, 李若楠, 王丽英 . 不同水氮用量对日光温室黄瓜季土壤硝态氮淋失的影响. 植物营养与肥料学报, 2012,18(3):645-653.
doi: 10.11674/zwyf.2012.11386 |
YAN P, WU X P, HUA L, WU Q F, LI Y K, WU H J, WANG X B, CAI D X, ZHANG Y C, LI R N, WANG L Y . Effects of different water and nitrogen application rates on soil nitrate-nitrogen leaching in cucumber greenhouse in solar greenhouse. Journal of Plant Nutrition and Fertilizer, 2012,18(3):645-653. (in Chinese)
doi: 10.11674/zwyf.2012.11386 |
|
[7] | 武其甫, 武雪萍, 李银坤, 吴会军, 闫鹏, 张彦才, 李若楠, 王丽英, 王小彬, 蔡典雄 . 保护地土壤N2O排放通量特征研究. 植物营养与肥料学报, 2011,17(04):942-948. |
WU Q F, WU X P, LI Y K WU H , JYAN P, ZHANG Y C, LI R N, WANG L Y, WANG X B, CAI D X . Characteristics of N2O emission flux in protected soils. Journal of Plant Nutrition and Fertilizer, 2011,17(4):942-948. (in Chinese) | |
[8] | 刘全国, 张义勇 . 北方日光温室生产存在的问题及对策. 北方园艺, 2006(1):74-75. |
LIU Q G, ZAHNG Y Y . Problems and countermeasures in the production of solar greenhouses in North China. Northern Horticulture, 2006(1):74-75. (in Chinese) | |
[9] | 刘兆辉, 江丽华, 张文君, 郑福丽, 王梅, 林海涛 . 山东省设施蔬菜施肥量演变及土壤养分变化规律. 土壤学报, 2008(2):296-303. |
LIU Z H, JIANG L H, ZHANG W J, ZHENG F L, WANG M, LIN H T . Evolution of fertilization amount and soil nutrient variation in facility vegetables in Shandong Province. Journal of Soil Science, 2008(2):296-303. (in Chinese) | |
[10] | 刘丽鹃 . 有机无机配施对大棚和露地蔬菜生长及土壤性状和温室气体排放的影响[D]. 南京: 南京农业大学, 2013. |
LIU L J . Effects of organic and inorganic application on growth and soil properties and greenhouse gas emissions of greenhouse and open field vegetables[D]. Nanjing: Nanjing Agricultural University, 2013. ( in Chinese) | |
[11] | PALMER I, PFAB H, RUSER R, FIEDLER S. Nitrogen loss from high N-input vegetable fields: a) Direct N2O emissions b) Spatiotemporal variability of N species (N2O, NH4 +, NO3 -) in soils [C]// EGU General Assembly Conference. EGU General Assembly Conference Abstracts, 2009. |
[12] | MOSIER A R, HEINEMEYER O . Current methods used to estimate N2O and N2 emissions from field soils[M]// Denitrification in the Nitrogen Cycle. Springer US, 1985. |
[13] | 王颀, 吴春涛, 李丹丹, 王海光, 毕焕改, 艾希珍 . 水肥一体化模式下日光温室黄瓜氮磷钾优化施肥方案的研究. 园艺学报, 2018,45(4):764-774. |
WANG Q, WU C T, LI D D, WANG H G, BI H G, AI X Z . Study on optimized fertilization of nitrogen, phosphorus and potassium in solar greenhouse under water and fertilizer Integration model. Journal of Horticulture, 2018,45(4):764-774. (in Chinese) | |
[14] |
李若楠, 武雪萍, 张彦才, 王丽英, 李孝兰, 陈丽莉, 翟凤芝 . 滴灌氮肥用量对设施菜地硝态氮含量及环境质量的影响. 植物营养与肥料学报, 2015,21(6):1642-1651.
doi: 10.11674/zwyf.2015.0632 |
LI R N, WU X P, ZHANG Y C, WANG L Y, LI X L, CHEN L L, ZHAI F Z . Effects of nitrogen application rate of drip irrigation on nitrate nitrogen content and environmental quality in greenhouse vegetable fields. Journal of Plant Nutrition and Fertilizer, 2015(6):1642-1651. (in Chinese)
doi: 10.11674/zwyf.2015.0632 |
|
[15] | 刘素慧, 刘世琦, 张自坤, 尉辉, 齐建建, 段吉锋 . 大蒜连作对其根际土壤微生物和酶活性的影响[J]. 中国农业科学, 2010,43(05):1000-1006. |
LIU S H, LIU S Q, ZHANG Z K, WEI H, QI J J . Effects of continuous cropping of garlic on microbial and enzyme activities in rhizosphere Soil[J]. Chinese Agricultural Science, 2010,43(5):1000-1006. (in Chinese) | |
[16] | WRAGE N, GROENIGEN J W V, OENEMA O, BAGGS E M . A novel dual-isotope labelling method for distinguishing between soil sources of N2O. Rapid Communications in Mass Spectrometry, 2005,19(22):3298-3306. |
[17] | 朱永官, 王晓辉, 杨小茹, 徐会娟, 贾炎 . 农田土壤N2O产生的关键微生物过程及减排措施. 环境科学, 2014,1(2):792-800. |
ZHU Y G, WANG X H, YANG X R, XU H J, JIA Y . Key microbial processes and emission reduction measures for N2O production in farmland soils. Environmental Science, 2014,1(2):792-800. (in Chinese) | |
[18] | 王苏平, 辉建春, 林立金, 朱雪梅, 朱波 . 施肥制度对川中丘陵区玉米不同生育期土壤反硝化酶活性的影响. 水土保持研究, 2012,19(3):274-283. |
WANG S P, HUI J C, LIN L J, ZHU X M, ZHU B . Effects of fertilization system on soil denitrifying enzyme activities in Different growth stages of maize in hilly areas of central Sichuan. Soil and Water Conservation Research, 2012,19(3):274-283. (in Chinese) | |
[19] | KUNC F. Soil enzymes// BURNS R G. Folia Microbiologica. London - New York- San Francisco: Academic Press, 1978. |
[20] | 徐国伟, 段骅, 王志琴, 刘立军, 杨建昌 . 麦秸还田对土壤理化性质及酶活性的影响. 中国农业科学, 2009,42(3):934-942. |
XU G W, DUAN H, WANG Z Q, LIU L J, YANG C J . Effects of wheat straw returning on soil physical and chemical properties and enzyme activity. Chinese Agricultural Science, 2009,42(3):934-942. (in Chinese) | |
[21] |
李银坤, 郭文忠, 薛绪掌, 乔晓军, 王利春, 陈红, 赵倩, 陈菲 . 不同灌溉施肥模式对温室番茄产量、品质及水肥利用的影响. 中国农业科学, 2017,50(19):3757-3765.
doi: 10.3864/j.issn.0578-1752.2017.19.012 |
LI Y K, GUO W Z, XUE X Z, QIAO X J, WANG L C, CHEN H, ZHAO Q, CHENG F . Effects of different fertigation patterns on yield, quality and water and fertilizer utilization of greenhouse tomatoes. Scientia Agricultura Sinica, 2017,50(19):3757-3765. (in Chinese)
doi: 10.3864/j.issn.0578-1752.2017.19.012 |
|
[22] | 于舜章 . 山东省设施黄瓜水肥一体化滴灌技术应用研究. 水资源与水工程学报, 2009,20(6):173-176. |
YU S Z . Application research of integrated irrigation and drip irrigation technology for cucumber in Shandong Province. Journal of Water Resources and Water Engineering, 2009,20(6):173-176. (in Chinese) | |
[23] | 强浩然, 张国斌, 郁继华, 马国礼, 张柏杨, 季磊, 王翠丽, 叶洁, 杜淼鑫 . 不同水分和氮素供应对日光温室辣椒栽培基质氮转化细菌和酶活性的影响. 园艺学报, 2018,45(5):943-958. |
QIANG H R, ZHANG G B, YU J H, MA G L, ZHANG B Y, JI L, WANG C L, YE J, DU M X . Effects of different water and nitrogen supply on nitrogen-transformed bacteria and enzyme activities in pepper greenhouse cultivation in solar greenhouse. Acta Horticulturae Sinica, 2018,45(5):943-95. (in Chinese) | |
[24] | 方泽涛, 王楷 . 不同灌溉模式和施氮处理稻田N2O排放与反硝化酶活性的关系. 应用与环境生物学报, 2017(6):1059-1066. |
FANG Z T, WANG K . Relationship between N2O emission and denitrifying enzyme activity in different irrigation modes and nitrogen treatment. Journal of Applied and Environmental Biology, 2017(6):1059-1066. (in Chinese) | |
[25] | 和文祥, 魏燕燕, 蔡少华 . 土壤反硝化酶活性测定方法及影响因素研究. 西北农林科技大学学报(自然科学版), 2006(1):121-124. |
HE W X, WEI Y Y, CAI S H . Determination of soil denitrifying enzyme activity and its influencing factors. Journal of Northwest A&F University (Natural Science Edition), 2006(1):121-124. (in Chinese) | |
[26] |
陈欢, 李玮, 张存岭, 乔玉强, 杜世州, 赵竹, 曹承富 . 淮北砂姜黑土酶活性对长期不同施肥模式的响应. 中国农业科学, 2014,47(3):495-502.
doi: 10.3864/j.issn.0578-1752.2014.03.009 |
CHEN H, LI W, ZHANG C L, QIAO Y Q, DU S Z, ZHAO Z, CAO C F . Response of enzyme activity of Shajiang black soil in Huaibei to long-term different fertilization models. Scientia Agricultura Sinica, 2014,47(3):495-502. (in Chinese)
doi: 10.3864/j.issn.0578-1752.2014.03.009 |
|
[27] | 史奕, 黄国宏 . 土壤中反硝化酶活性变化与N2O排放的关系. 应用生态学报, 1999(03):74-76. |
SHI Y, HUANG G H . Relationship between changes of soil denitrifying enzyme activity and N2O emission in soil. Chinese Journal of Applied Ecology, 1999(03):74-76. (in Chinese) | |
[28] | 鲁亚楠, 李开忠, 吕艳杰, 曹玉军 . 水氮互作下土壤脲酶活性与N2O排放的相关性研究. 吉林农业科技学院学报, 2015,24(4):24-27, 32. |
LU Y N, LI K Z, LV Y J, CAO Y J . Correlation between soil urease activity and N2O emission under water-nitrogen interaction. Journal of Jilin Agricultural Science and Technology, 2015,24(4):24-27, 32. (in Chinese) | |
[29] | 李华, 陈英旭, 梁新强, 田光明, 俞巧钢 . 土壤脲酶活性对稻田田面水氮素转化的影响. 水土保持学报, 2006(1):55-58. |
LI H, CHEN Y X, LIANG X Q, TIAN G M, YU Q G . Effects of soil urease activity on nitrogen transformation in rice fields. Journal of Soil and Water Conservation, 2006(1):55-58. (in Chinese) | |
[30] | 关松荫, 孟昭鹏 . 不同垦殖年限黑土农化性状与酶活性的变化. 土壤通报, 1986(4):157-159. |
GUAN S Y, MENG Z P . Agrochemical characters and enzyme activities of black soils with different Years of reclamation. Chinese Journal of Soil Science, 1986(4):157-159. (in Chinese) | |
[31] | 史云峰, 武志杰, 史奕, 陈利军, 王殳屹 . 土壤羟胺还原酶活性测定方法的改进. 生态学杂志, 2007(7):1133-1137. |
SHI Y F, WU Z J, SHI Y, CHEN L J, WANG S Y . Improvement of determination method of soil hydroxylamine reductase activity. Chinese Journal of Ecology, 2007(7):1133-1137. (in Chinese) | |
[32] | 王立刚, 李虎, 邱建军 . 黄淮海平原典型农田土壤N2O的排放特征. 中国农业科学, 2008,41(4):1248-1254. |
WANG L G, LI H, QIU J J . Emission characteristics of N2O from typical farmland soils in the Huang-Huai-Hai Plain. Scientia Agricultura Sinica, 2008,41(4):1248-1254. (in Chinese) | |
[33] | CUI Z, YUE S, WANG G, MENG Q, WU L, YANG Z, ZHANG Q, LI S, ZHANG F, CHEN X . Closing the yield gap could reduce projected greenhouse gas emissions: a case study of maize production in China. Global Change Biology, 2013,19(8):2467-2477. |
[34] | XIONG Z Q, XIE Y X, XING G X, ZHUA Z L, CHRIS B . Measurements of nitrous oxide emissions from vegetable production in China. Atmospheric Environment, 2006,40(12):2225-2234. |
[35] | HE F, JIANG R, CHRN Q, ZHANG F, FU F . Nitrous oxide emissions from an intensively managed greenhouse vegetable cropping system in Northern China. Environmental Pollution, 2009,157(5):1666-1672. |
[36] | WANG X, YANG X, ZHANG Z, YE X, KAO C M, CHEN S . Long-term effect of temperature on N2O emission from the denitrifying activated sludge. Journal of Bioscience & Bioengineering, 2014,117(3):298-304. |
[37] | DOBBIE K E, SMITH K A . Nitrous oxide emission factors for agricultural soils in Great Britain: the impact of soil water‐filled pore space and other controlling variables. Global Change Biology, 2010,9(2):204-218. |
[38] | 汪俊玉, 刘东阳, 宋霄君, 武雪萍, 李晓秀, 黄绍文, 李若楠 . 滴灌水肥一体化条件下番茄氮肥适宜用量探讨. 中国土壤与肥料, 2018(6):98-103. |
WANG J Y, LIU D Y, SONG X J, WU X P, LI X X, HUANG S W, LI R N . Discussion on the suitable amount of nitrogen fertilizer for tomato under the condition of integrated drip irrigation with water and fertilizer. Soil and Fertilizer Sciences in China, 2018(6):98-103. (in Chinese) | |
[39] | 黄绍文, 唐继伟, 殷学云, 张怀志, 袁硕 . 基于发育阶段的日光温室有机基质栽培番茄水肥一体化技术. 中国果菜, 2017,37(9):52-54, 60. |
HUANG S W, TANG J W, YIN X Y, ZHANG H Z, YUAN S . Integrated water and fertilizer technology for tomato cultivation in solar greenhouse based on developmental stage. Chinese Fruit and Vegetable, 2017,37(9):52-54, 60. (in Chinese) | |
[40] | 郝小雨, 高伟, 王玉军, 金继运, 黄绍文, 唐继伟, 张志强 . 有机无机肥料配合施用对设施菜田土壤N2O排放的影响. 植物营养与肥料学报, 2012,18(5):1073-1085. |
HAO X Y, GAO W, WANG Y J, JIN J Y, HUANG S W, TANG J W, ZHANG Z Q . Effects of combined application of organic and inorganic fertilizers on soil N2O emissions from greenhouse vegetable fields. Journal of Plant Nutrition and Fertilizer, 2012,18(5):1073-1085. (in Chinese) | |
[41] | 陈海燕, 李虎, 王立刚, 邱建军 . 京郊典型设施蔬菜地N2O排放规律及影响因素研究. 中国土壤与肥料, 2012(5):5-10. |
CHEN H Y, LI H, WANG L G, QIU J J . Study on N2O emissions and influencing factors of typical vegetable areas in Beijing Suburbs. Soil and Fertilizer Sciences in China, 2012(5):5-10. (in Chinese) | |
[42] |
张婧, 李虎, 王立刚, 邱建军 . 京郊典型设施蔬菜地土壤N2O排放特征. 生态学报, 2014,34(14):4088-4098.
doi: 10.5846/stxb201306091534 |
ZHANG J, LI H, WANG L G, QIU J J . Characteristics of soil N2O emission from typical vegetable fields in Beijing suburbs. Chinese Journal of Ecology, 2014,34(14):4088-4098. (in Chinese)
doi: 10.5846/stxb201306091534 |
|
[43] | SMITH K A, THOMSON P E, CLAYTON H, IAIN M . Effects of temperature, water content and nitrogen fertilisation on emissions of nitrous oxide by soils. Atmospheric Environment, 1998,32(19):3301-3309. |
[44] |
郑欠, 丁军军, 李玉中, 林伟, 徐春英, 李巧珍, 毛丽丽 . 土壤含水量对硝化和反硝化过程N2O排放及同位素特征值的影响. 中国农业科学, 2017,50(24):4747-4758.
doi: 10.3864/j.issn.0578-1752.2017.24.008 |
ZHENG Q, DING J J, LI Y Z, LIN W, XU C Y, LI Q Z, MAO L L . Effects of soil water content on N2O emission and isotope characteristics of nitrification and denitrification processes. Scientia Agricultura Sinica, 2017,50(24):4747-4758. (in Chinese)
doi: 10.3864/j.issn.0578-1752.2017.24.008 |
|
[45] | 丁洪, 王跃思, 项虹艳, 李卫华 . 菜田氮素反硝化损失与N2O排放的定量评价. 园艺学报, 2004(6):762-766. |
DING H, WANG Y S, XIANG H Y, LI W H . Quantitative evaluation of nitrogen denitrification loss and N2O emission in vegetable fields. Acta Horticulturae Sinica, 2004(6):762-766. (in Chinese) | |
[46] |
王艳丽, 李虎, 孙媛, 王立刚 . 水肥一体化条件下设施菜地的N2O排放. 生态学报, 2016,36(7):2005-2014.
doi: 10.5846/stxb201409301932 |
WANG Y L, LI H, SUN Y, WANG L G . N2O emissions from protected vegetable fields under water and fertilizer integration conditions. Acta Ecologica Sinica, 2016,36(7):2005-2014. (in Chinese)
doi: 10.5846/stxb201409301932 |
|
[47] | DIAO T T, XIE L L, GUO L P, YAN H L . Measurements of N2O emissions from different vegetable fields on the North China Plain. Atmospheric Environment, 2013,72(2):70-76. |
[48] | 巨晓棠, 张福锁 . 关于氮肥利用率的思考. 生态环境, 2003(2):192-197. |
JU X T, ZHANG F S . Thinking on the utilization ratio of nitrogen fertilizer. Ecological Environment, 2003(2):192-197. (in Chinese) | |
[49] | 张光亚, 方柏山, 闵航, 陈美慈 . 设施栽培土壤氧化亚氮排放及其影响因子的研究. 农业环境科学学报, 2004(1):144-147. |
ZHANG G Y, FANG B S, MIN H, CHEN M C . Study on nitrous oxide emission from soil in greenhouse cultivation and its impact factors. Journal of Agro-Environment Science, 2004,23(1):144-147. (in Chinese) | |
[50] | 姚志生, 郑循华, 周再兴, 谢宝华, 梅宝玲, 顾江新, 王定勇 . 太湖地区冬小麦田与蔬菜地N2O排放对比观测研究. 气候与环境研究, 2006(6):691-701. |
YAO Z S, ZHENG X H, ZHOU Z X, XIE B H, MEI B L, GU J X, WANG D Y . Comparative observation of N2O emissions from winter wheat fields and vegetable fields in Taihu Lake Region. Climatic and Environmental Research, 2006,11(6):691-701. (in Chinese) | |
[51] | 徐文彬, 洪业汤 . 贵州省旱田土壤N2O释放及其环境影响因素. 环境科学, 2000,21(1):7-11. |
XU W B, HONG Y T . N2O emission from dryland soils in Guizhou Province and its environmental impact factors. Environmental Science, 2000,21(1):7-11. (in Chinese) | |
[52] | 郑循华, 王明星 . 温度对农田N2O产生与排放的影响. 环境科学, 1997(5):1-5. |
ZHENG X H, WANG M X . Effects of temperature on N2O production and emission in farmland. Environmental Science, 1997(5):1-5. (in Chinese) | |
[53] |
张恩平, 谭福雷, 王月, 张淑红, 段瑜, 周芳 . 氮磷钾与有机肥配施对番茄产量品质及土壤酶活性的影响. 园艺学报, 2015,42(10):2059-2067.
doi: 10.16420/j.issn.0513-353x.2015-0341 |
ZHANG E P, TAN F L, WANG Y, ZHANG S H, DUAN Y, ZHOU F . Effects of nitrogen, phosphorus, potassium and organic fertilizers on yield and quality of tomato and soil enzyme activities. Acta Horticulturae Sinica, 2015,42(10):2059-2067. (in Chinese)
doi: 10.16420/j.issn.0513-353x.2015-0341 |
|
[54] | DICK W A . Influence of long-term tillage and crop rotation combinations on soil enzyme activities. Soil Science Society of America Journal, 1984,48(3):569-574. |
[55] | 孙建, 刘苗, 李立军, 刘景辉, 张星杰 . 免耕与留茬对土壤微生物量C、N及酶活性的影响. 生态学报, 2009,29(10):5508-5515. |
SUN J, LIU M, LI L J, LIU J H, ZHANG X J . Effects of no-tillage and stubble on soil microbial biomass C, N and enzyme activities. Acta Ecologica Sinica, 2009,29(10):5508-5515. (in Chinese) | |
[56] | 李东坡, 武志杰, 陈利军, 杨杰, 朱平, 任军, 彭畅, 高红军 . 长期培肥黑土脲酶活性动态变化及其影响因素. 应用生态学报, 2003(12):2208-2212. |
LI D P, WU Z J, CHEN L J, YANG J, ZHU P, PENG C, GAO H J . Dynamic changes of urease activity in long-term fertilization black soil and its influencing factors. Chinese Journal of Applied Ecology, 2003(12):2208-2212. (in Chinese) | |
[57] | 王栋, 李辉信, 胡锋 . 不同耕作方式下覆草旱作稻田土壤肥力特征. 土壤学报, 2011,48(6):1203-1209. |
WANG D, LI H X, HU F . Characteristics of soil fertility in paddy field under different tillage patterns. Acta Pedologica Sinica, 2011,48(6):1203-1209. (in Chinese) | |
[58] |
王树起, 韩晓增, 乔云发, 王守宇, 李晓慧 . 不同土地利用和施肥方式对土壤酶活性及相关肥力因子的影响. 植物营养与肥料学报, 2009,15(6):1311-1316.
doi: 10.11674/zwyf.2009.0610 |
WANG S Q, HAN X Z, QIAO Y F, WANG S Y, LI X H . Effects of different land use and fertilization methods on soil enzyme activities and related fertility factors. Journal of Plant Nutrition and Fertilizer, 2009,15(6):1311-1316. (in Chinese)
doi: 10.11674/zwyf.2009.0610 |
|
[59] | 褚素贞, 张乃明, 史静, 毛昆明 . 云南省设施栽培土壤脲酶活性变化趋势研究. 土壤通报, 2010,41(4):811-814. |
ZHU S Z, ZHANG N M, SHI J, MAO K M . Study on the change trend of soil urease activity in protected cultivation in Yunnan Province. Chinese Journal of Soil Science, 2010,41(4):811-814. (in Chinese) | |
[60] | 肖新, 朱伟, 肖靓, 邓艳萍, 赵言文, 汪建飞 . 适宜的水氮处理提高稻基农田土壤酶活性和土壤微生物量碳氮. 农业工程学报, 2013,29(21):91-98. |
XIAO X, ZHU W, XIAO L, DENG Y P, ZHAO Y W, WANG J F . Appropriate water and nitrogen treatment to increase soil enzyme activity and soil microbial biomass carbon and nitrogen in rice-based farmland. Transactions of the Chinese Society of Agricultural Engineering, 2013,29(21):91-98. (in Chinese) | |
[61] | 夏雪, 谷洁, 车升国, 高华, 秦清军 . 施氮水平对塿土微生物群落和酶活性的影响. 中国农业科学, 2011,44(8):1618-1627. |
XIA X, GU J, CHE S G, GAO H, QIN Q J . Effects of nitrogen application levels on microbial community and enzyme activities in bauxite. Scientia Agricultura Sinica, 2011,44(8):1618-1627. (in Chinese) | |
[62] | 白红英, 韩建刚, 赵一萍 . 不同土层土壤理化性状与反硝化酶活性N2O排放通量的相关性研究. 农业环境保护, 2002,21(3):193-196. |
BAI H Y, HAN J G, ZHAO Y P . Correlation between physical and chemical properties of different soil layers and denitrifying enzyme activity N2O emission flux. Agro- environmental Protection, 2002,21(3):193-196. (in Chinese) | |
[63] | 周礼恺 . 土壤的酶活性. 土壤学进展, 1980,8(4):9-15. |
ZHOU L K . Soil enzyme activity. Advances in Soil Science, 1980,8(4):9-15. (in Chinese) | |
[64] | 陈利军, 武志杰, 姜勇, 周礼恺 . 与氮转化有关的土壤酶活性对抑制剂施用的响应. 应用生态学报, 2002(9):1099-1103. |
CHEN L J, WU Z J, JIANG Y, ZHOU L K . Response of soil enzyme activity related to nitrogen conversion to inhibitor application. Chinese Journal of Applied Ecology, 2002(9):1099-1103. (in Chinese) | |
[65] | KNOWLES R . Denitrification. Microbiology Reviews, 1982,46(1):43-70. |
[66] | 宋琴, 许雷 . 异养硝化作用酶学研究进展. 生物技术通报, 2008(5):60-62. |
SONG Q, XU L . Advances in enzymology of heterotrophic nitrification. Biotechnology Bulletin, 2008(5):60-62. (in Chinese) |
[1] | 古丽旦,刘洋,李方向,成卫宁. 小麦吸浆虫小热激蛋白基因Hsp21.9的克隆及在滞育过程与温度胁迫下的表达特性[J]. 中国农业科学, 2023, 56(1): 79-89. |
[2] | 王树会,陶雯,梁硕,张旭博,孙楠,徐明岗. 长期施用有机肥情景下华北平原旱地土壤固碳及N2O排放的空间格局[J]. 中国农业科学, 2022, 55(6): 1159-1171. |
[3] | 刘振蓉,赵武奇,胡新中,贺刘成,陈月圆. 燕麦挂面制作过程中干燥工艺优化研究[J]. 中国农业科学, 2022, 55(24): 4927-4942. |
[4] | 万连杰,何满,李俊杰,田洋,张绩,郑永强,吕强,谢让金,马岩岩,邓烈,易时来. 有机肥替代部分化肥对椪柑生长、品质及土壤特性的影响[J]. 中国农业科学, 2022, 55(15): 2988-3001. |
[5] | 张锦源,李彦生,于镇华,谢志煌,刘俊杰,王光华,刘晓冰,吴俊江,Stephen J Herbert,金剑. 作物-土壤氮循环对大气CO2浓度和温度升高响应的研究进展[J]. 中国农业科学, 2021, 54(8): 1684-1701. |
[6] | 郑海霞,高玉林,张方梅,杨超霞,蒋健,朱勋,张云慧,李祥瑞. 马铃薯甲虫热激蛋白基因Ld-hsp70的克隆及温度胁迫下的表达特性[J]. 中国农业科学, 2021, 54(6): 1163-1175. |
[7] | 杨语嫣,李耀文,邢爽,张敏红,冯京海. 基于体表温度的肉鸡温湿指数模型研究[J]. 中国农业科学, 2021, 54(6): 1270-1279. |
[8] | 张丽媛,吕金东,石欣悦,虞娜,邹洪涛,张玉玲,张玉龙. 灌溉下限对设施土壤N2O和NO排放特征的影响[J]. 中国农业科学, 2021, 54(5): 992-1002. |
[9] | 黄明,吴金芝,李友军,付国占,赵凯男,张振旺,杨中帅,侯园泉. 耕作方式和氮肥用量对旱地小麦产量、蛋白质含量和土壤硝态氮残留的影响[J]. 中国农业科学, 2021, 54(24): 5206-5219. |
[10] | 王新媛,赵思达,郑险峰,王朝辉,何刚. 秸秆还田和氮肥用量对冬小麦产量和氮素利用的影响[J]. 中国农业科学, 2021, 54(23): 5043-5053. |
[11] | 曹寒冰,谢钧宇,刘菲,高健永,王楚涵,王仁杰,谢英荷,李廷亮. 地膜覆盖麦田土壤有机碳矿化特征及其温度敏感性[J]. 中国农业科学, 2021, 54(21): 4611-4622. |
[12] | 王国丽,常芳弟,张宏媛,卢闯,宋佳珅,王婧,逄焕成,李玉义. 不同厚度秸秆隔层对河套灌区盐碱土壤温度、水分和食葵产量的影响[J]. 中国农业科学, 2021, 54(19): 4155-4168. |
[13] | 马悦,田怡,苑爱静,王浩琳,李永华,黄婷苗,黄宁,李超,党海燕,邱炜红,何刚,王朝辉,石美. 北方麦区小麦产量与蛋白质含量变化对土壤硝态氮的响应[J]. 中国农业科学, 2021, 54(18): 3903-3918. |
[14] | 柳艳霞,王振宇,郑晓春,朱瑶迪,陈丽,张德权. 基于品质指标预测北京烤鸭的中心温度[J]. 中国农业科学, 2020, 53(8): 1655-1663. |
[15] | 王钧,李广,闫丽娟,刘强,聂志刚. 旱地春小麦产量对不同生育阶段温度变化的响应模拟[J]. 中国农业科学, 2020, 53(5): 904-916. |
|