关键词: 芜菁, 拟南芥cDNA芯片, 雌蕊发育, 基因表达, 转录

Abstract:

【Objective】 The possible reasons of the pistil abortion in turnip have been studied at the transcriptional level and it will be helpful to provide some useful clues to illuminate the molecular genetics mechanism of the gynoecium development in Brassica crops. 【Method】 The Arabidopsis ATH1 genome array was employed to analyze the transcriptome differences between the tpa mutants and their wild type flowers. Subsequently, the RT-PCR was used to confirm the genes with transcriptional difference. 【Result】 Data analysis results indicated that there were differences in 152 genes between the wild type (W1) and the pistil aborted completely (M3), likewise, 61 genes with transcriptional difference between the W1and the pistil aborted partially (I2), and 24 genes with transcriptional difference between the I2 and M3. All of the previous genes were classified into the following in functional categoryies: cell wall biosynthesis and regulation, defense and stressing reaction, transcriptional regulation, protein metabolism and normal metabolism, respectively. Out of the 41 genes, 9 genes with significant temporal and spatial transcription difference (At2g42840, At1g57750, At5g20630, At2g03090, At3g08030, At5g08000, At2g28790, At5g63310, At2g24270) were obtained through the RT-PCR screening and confirmation. 【Conclusion】 The Arabidopsis ATH1 genome array can be successfully utilized to analyze the transcriptome differences between the tpa mutants and their wild type. And the 9 genes with significant temporal and spatial transcription difference, are not only important to the reproductive process including gynoecium development, but also involve in the vegetative processes.

Key words: Brassica campestris L. ssp. rapifera Sinsk, syn. B. rapa L. ssp. Rapifera, Arabidopsis ATH1 genome array, gynoecium development, gene expression, transcription