中国农业科学 ›› 2011, Vol. 44 ›› Issue (5): 874-883 .

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

红麻6个重要产量性状的QTL定位

陈美霞,祁建民,方平平,李爱青,危成林,陶爱芬,徐建堂,谢增荣,林培清,兰涛,吴建梅,陈富成

  

  1. (福建农林大学作物遗传育种与综合利用省部共建教育部重点实验室)
  • 收稿日期:2010-07-16 修回日期:2010-09-05 出版日期:2011-03-01 发布日期:2011-03-01
  • 通讯作者: 祁建民,方平平

QTL Mapping of Six Yield Traits in Kenaf

CHEN Mei-xia, QI Jian-min, FANG Ping-ping, LI Ai-qing, WEI Cheng-lin, TAO Ai-fen, XU Jian-tang,XIE Zeng-rong, LIN Pei-qing, LAN Tao, WU Jian-mei, CHEN Fu-cheng
  

  1. (福建农林大学作物遗传育种与综合利用省部共建教育部重点实验室)
  • Received:2010-07-16 Revised:2010-09-05 Online:2011-03-01 Published:2011-03-01
  • Contact: QI Jian-min, FANG Ping-ping

摘要:

【目的】研究红麻重要产量性状的QTL定位,促进红麻分子辅助育种基础科学研究。【方法】以埃及的阿联红麻与福建农林大学育成的高产抗病优质新品种福红992作为亲本进行杂交,自交衍生的162个F2﹕3家系为材料,通过一年两点的随机区组田间试验,测定了红麻株高、茎粗、节数、单株鲜皮重、单株干皮重和种子千粒重6个重要产量性状的数据,采用混合线性模型的复合区间作图法进行了QTL定位及其遗传互作效应分析。【结果】相关分析结果表明,除种子千粒重外,其它性状之间均存在明显的正相关性。在两地共定位了2个株高QTL、2个茎粗QTL、2个节数QTL、1个单株鲜皮重QTL、2个单株干皮重和2个种子千粒重QTL。【结论】在两地检测到11个QTL,主要集中分布在第6、11、14、9、13、17和4连锁群上,这些QTL在连锁群上分布不均匀,具有集中分布的特点。

关键词: 红麻, 产量性状, QTL

Abstract:

【Objective】 The objective of this study was to identify QTL controlling partial yield traits in kenaf which could promote the basic scientific research of molecular marker-assisted selection. 【Method】In this investigation, a population with 162 F2:3 individuals from the cross between Alian kenaf (from Egypt) and Fuhong 992 (from Fujian Agriculture and Forestry University) with fine qualities, high yield and disease resistance was used and two location field tests were conducted. A randomized complete block design was used, to charactererize six yield traits: plant height, stem diameter, number of nodes, fresh bark weight per plant, dry bark weight per plant, 1000-seed weight. By using the data of field tests and MCIM method, six important yield traits were located and their genetic interactions were analyzed. 【Result】 Correlation analysis of field performance indicated significant positive correlation among all the traits except 1000-seed weight. As a result, two QTLs of plant height, two QTLs of stem diameter, two QTLs of number of nodes, one QTL of fresh bark weight per plant, and two QTLs of dry bark weight per plant, and two QTLs of 1000-seed weight were mapped in two places. 【Conclusion】 The eleven QTLs identified mainly clustered in linkage groups 6, 11, 14, 9, 13, 17 and 4, and all of these QTL distributed unevenly on chromosomes and tended to cluster together.

Key words: kenaf (Hibiscus cannabinus L.), yield traits, quantitative trait loci (QTL)