关键词: 大豆, 大豆疫霉, 遗传分析, 抗性基因, SSR标记

Abstract:

【Objective】 The objective of this study was to reveal the inheritance pattern and map resistance gene to Phytophthora sojae (P. sojae) strain Pm14 in soybean.【Method】A population of 176 recombinant inbred lines (RIL) derived from the cross of Su88-M21×Xinyixiaoheidou was evaluated for phytophthora resistance using the hypocotyl inoculation technique. The RIL was used to analyze the inheritance of resistance and map the resistance gene to a linkage group.【Result】 The results showed that the resistance of Su88-M21 to strain Pm14 is controlled by a single gene (Rps), and the resistance is dominant to susceptible. RpsSu (resistance gene in Su88-M21 was temporarily designated) is a novel phytophthora resistance gene which was firstly found on molecular linkage group O, and flanked by Satt358 and Sat_242 with genetic distances 3.5 cM and 7.4 cM.【Conclusion】The resistance to Pm14 is controlled by a single dominant gene (Rps), and the resistance gene RpsSu in Su88-M21 was firstly mapped on molecular linkage group O.

Key words: soybean, Phytophthora sojae, genetic analysis, resistance gene, SSR marker